Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rtcA and OKFHMN_16960 are separated by 49 nucleotides OKFHMN_16960 and OKFHMN_16965 overlap by 10 nucleotides OKFHMN_16965 and malT are separated by 66 nucleotides
OKFHMN_16955: rtcA - RNA 3'-terminal phosphate cyclase, at 3,305,284 to 3,306,312
rtcA
OKFHMN_16960: OKFHMN_16960 - GNAT family N-acetyltransferase, at 3,306,362 to 3,306,847
_16960
OKFHMN_16965: OKFHMN_16965 - DUF1778 domain-containing protein, at 3,306,838 to 3,307,116
_16965
OKFHMN_16970: malT - HTH-type transcriptional regulator MalT, at 3,307,183 to 3,309,888
malT
Position (kb)
3306
3307
3308 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3305.871 kb on - strand, within rtcA at 3305.871 kb on - strand, within rtcA at 3305.873 kb on + strand, within rtcA at 3306.146 kb on + strand, within rtcA at 3306.183 kb on + strand, within rtcA at 3306.183 kb on + strand, within rtcA at 3306.184 kb on - strand, within rtcA at 3306.358 kb on + strand at 3306.358 kb on + strand at 3306.358 kb on + strand at 3306.364 kb on - strand at 3306.381 kb on + strand at 3306.382 kb on - strand at 3306.387 kb on - strand at 3306.391 kb on + strand at 3306.391 kb on + strand at 3306.392 kb on - strand at 3306.392 kb on - strand at 3306.413 kb on + strand, within OKFHMN_16960 at 3306.413 kb on + strand, within OKFHMN_16960 at 3306.413 kb on + strand, within OKFHMN_16960 at 3306.413 kb on + strand, within OKFHMN_16960 at 3306.414 kb on - strand, within OKFHMN_16960 at 3306.485 kb on + strand, within OKFHMN_16960 at 3306.551 kb on - strand, within OKFHMN_16960 at 3306.584 kb on + strand, within OKFHMN_16960 at 3306.587 kb on + strand, within OKFHMN_16960 at 3306.616 kb on + strand, within OKFHMN_16960 at 3306.616 kb on + strand, within OKFHMN_16960 at 3306.617 kb on - strand, within OKFHMN_16960 at 3306.643 kb on + strand, within OKFHMN_16960 at 3306.643 kb on + strand, within OKFHMN_16960 at 3306.644 kb on - strand, within OKFHMN_16960 at 3306.652 kb on + strand, within OKFHMN_16960 at 3306.662 kb on - strand, within OKFHMN_16960 at 3306.672 kb on + strand, within OKFHMN_16960 at 3306.672 kb on + strand, within OKFHMN_16960 at 3306.684 kb on - strand, within OKFHMN_16960 at 3306.703 kb on + strand, within OKFHMN_16960 at 3306.704 kb on - strand, within OKFHMN_16960 at 3306.709 kb on + strand, within OKFHMN_16960 at 3306.808 kb on + strand at 3306.809 kb on - strand at 3306.829 kb on + strand at 3306.829 kb on + strand at 3306.829 kb on + strand at 3306.830 kb on - strand at 3306.856 kb on - strand at 3306.856 kb on - strand at 3306.971 kb on - strand, within OKFHMN_16965 at 3306.971 kb on - strand, within OKFHMN_16965 at 3307.083 kb on + strand, within OKFHMN_16965 at 3307.116 kb on + strand at 3307.145 kb on + strand at 3307.145 kb on + strand at 3307.150 kb on + strand at 3307.161 kb on + strand at 3307.162 kb on - strand at 3307.162 kb on - strand at 3307.176 kb on + strand at 3307.177 kb on - strand at 3307.177 kb on - strand at 3307.497 kb on + strand, within malT at 3307.599 kb on + strand, within malT at 3307.659 kb on + strand, within malT at 3307.660 kb on - strand, within malT at 3307.677 kb on - strand, within malT at 3307.687 kb on + strand, within malT at 3307.913 kb on + strand, within malT at 3307.914 kb on - strand, within malT at 3308.082 kb on - strand, within malT at 3308.082 kb on - strand, within malT
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,305,871 - rtcA OKFHMN_16955 0.57 -0.5 3,305,871 - rtcA OKFHMN_16955 0.57 -0.3 3,305,873 + rtcA OKFHMN_16955 0.57 -1.3 3,306,146 + rtcA OKFHMN_16955 0.84 +0.8 3,306,183 + rtcA OKFHMN_16955 0.87 -0.9 3,306,183 + rtcA OKFHMN_16955 0.87 +0.0 3,306,184 - rtcA OKFHMN_16955 0.87 +0.8 3,306,358 + -0.5 3,306,358 + +0.2 3,306,358 + +0.0 3,306,364 - +0.5 3,306,381 + -0.0 3,306,382 - -0.5 3,306,387 - +0.2 3,306,391 + -0.3 3,306,391 + +0.4 3,306,392 - +0.8 3,306,392 - +0.3 3,306,413 + OKFHMN_16960 0.10 +0.2 3,306,413 + OKFHMN_16960 0.10 +0.4 3,306,413 + OKFHMN_16960 0.10 +1.9 3,306,413 + OKFHMN_16960 0.10 +0.1 3,306,414 - OKFHMN_16960 0.11 -0.1 3,306,485 + OKFHMN_16960 0.25 +0.3 3,306,551 - OKFHMN_16960 0.39 -0.4 3,306,584 + OKFHMN_16960 0.46 +0.6 3,306,587 + OKFHMN_16960 0.46 +0.8 3,306,616 + OKFHMN_16960 0.52 -1.1 3,306,616 + OKFHMN_16960 0.52 +0.2 3,306,617 - OKFHMN_16960 0.52 +1.4 3,306,643 + OKFHMN_16960 0.58 +0.2 3,306,643 + OKFHMN_16960 0.58 -0.8 3,306,644 - OKFHMN_16960 0.58 -0.3 3,306,652 + OKFHMN_16960 0.60 +0.5 3,306,662 - OKFHMN_16960 0.62 -0.3 3,306,672 + OKFHMN_16960 0.64 +1.6 3,306,672 + OKFHMN_16960 0.64 -0.8 3,306,684 - OKFHMN_16960 0.66 +0.6 3,306,703 + OKFHMN_16960 0.70 -0.1 3,306,704 - OKFHMN_16960 0.70 +0.7 3,306,709 + OKFHMN_16960 0.71 +0.6 3,306,808 + -0.7 3,306,809 - -0.2 3,306,829 + +0.1 3,306,829 + +0.3 3,306,829 + -0.3 3,306,830 - -1.2 3,306,856 - -2.8 3,306,856 - -3.9 3,306,971 - OKFHMN_16965 0.48 -1.8 3,306,971 - OKFHMN_16965 0.48 -1.6 3,307,083 + OKFHMN_16965 0.88 +0.7 3,307,116 + -0.5 3,307,145 + +1.5 3,307,145 + -0.8 3,307,150 + +0.6 3,307,161 + +0.3 3,307,162 - -0.0 3,307,162 - -1.6 3,307,176 + +0.1 3,307,177 - +0.3 3,307,177 - +0.0 3,307,497 + malT OKFHMN_16970 0.12 -0.2 3,307,599 + malT OKFHMN_16970 0.15 +0.2 3,307,659 + malT OKFHMN_16970 0.18 +0.7 3,307,660 - malT OKFHMN_16970 0.18 -0.0 3,307,677 - malT OKFHMN_16970 0.18 +0.4 3,307,687 + malT OKFHMN_16970 0.19 +0.8 3,307,913 + malT OKFHMN_16970 0.27 -0.4 3,307,914 - malT OKFHMN_16970 0.27 -0.0 3,308,082 - malT OKFHMN_16970 0.33 +0.7 3,308,082 - malT OKFHMN_16970 0.33 +1.5
Or see this region's nucleotide sequence