Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rtcR and rtcB are separated by 187 nucleotides rtcB and rtcA are separated by 3 nucleotides
OKFHMN_16945: rtcR - DNA-binding transcriptional regulator RtcR, at 3,302,268 to 3,303,866
rtcR
OKFHMN_16950: rtcB - RNA-splicing ligase RtcB, at 3,304,054 to 3,305,280
rtcB
OKFHMN_16955: rtcA - RNA 3'-terminal phosphate cyclase, at 3,305,284 to 3,306,312
rtcA
Position (kb)
3304
3305
3306 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3303.152 kb on + strand, within rtcR at 3303.175 kb on - strand, within rtcR at 3303.183 kb on + strand, within rtcR at 3303.184 kb on - strand, within rtcR at 3303.185 kb on + strand, within rtcR at 3303.320 kb on + strand, within rtcR at 3303.375 kb on - strand, within rtcR at 3303.377 kb on - strand, within rtcR at 3303.377 kb on - strand, within rtcR at 3303.384 kb on + strand, within rtcR at 3303.384 kb on + strand, within rtcR at 3303.456 kb on + strand, within rtcR at 3303.545 kb on - strand, within rtcR at 3303.601 kb on + strand, within rtcR at 3303.690 kb on - strand, within rtcR at 3303.690 kb on - strand, within rtcR at 3303.768 kb on + strand at 3303.768 kb on + strand at 3303.769 kb on - strand at 3303.771 kb on - strand at 3303.771 kb on - strand at 3303.821 kb on - strand at 3303.821 kb on - strand at 3303.861 kb on - strand at 3303.870 kb on - strand at 3303.879 kb on + strand at 3303.880 kb on - strand at 3303.881 kb on + strand at 3303.892 kb on - strand at 3303.947 kb on + strand at 3304.146 kb on + strand at 3304.179 kb on + strand, within rtcB at 3304.180 kb on - strand, within rtcB at 3304.199 kb on + strand, within rtcB at 3304.199 kb on + strand, within rtcB at 3304.199 kb on + strand, within rtcB at 3304.200 kb on - strand, within rtcB at 3304.200 kb on - strand, within rtcB at 3304.302 kb on - strand, within rtcB at 3304.302 kb on - strand, within rtcB at 3304.402 kb on - strand, within rtcB at 3304.516 kb on - strand, within rtcB at 3304.516 kb on - strand, within rtcB at 3304.516 kb on - strand, within rtcB at 3304.522 kb on + strand, within rtcB at 3304.522 kb on + strand, within rtcB at 3304.523 kb on - strand, within rtcB at 3304.523 kb on - strand, within rtcB at 3304.523 kb on - strand, within rtcB at 3304.523 kb on - strand, within rtcB at 3304.523 kb on - strand, within rtcB at 3304.523 kb on - strand, within rtcB at 3304.523 kb on - strand, within rtcB at 3304.524 kb on + strand, within rtcB at 3304.524 kb on + strand, within rtcB at 3304.525 kb on - strand, within rtcB at 3304.599 kb on + strand, within rtcB at 3304.645 kb on + strand, within rtcB at 3304.720 kb on - strand, within rtcB at 3304.735 kb on + strand, within rtcB at 3304.900 kb on + strand, within rtcB at 3304.900 kb on + strand, within rtcB at 3304.937 kb on - strand, within rtcB at 3305.092 kb on - strand, within rtcB at 3305.191 kb on + strand at 3305.191 kb on + strand at 3305.192 kb on - strand at 3305.199 kb on + strand at 3305.200 kb on - strand at 3305.200 kb on - strand at 3305.241 kb on + strand at 3305.241 kb on + strand at 3305.245 kb on + strand at 3305.245 kb on + strand at 3305.245 kb on + strand at 3305.246 kb on - strand at 3305.253 kb on - strand at 3305.253 kb on - strand at 3305.270 kb on + strand at 3305.270 kb on + strand at 3305.278 kb on + strand at 3305.382 kb on + strand at 3305.383 kb on - strand at 3305.577 kb on + strand, within rtcA at 3305.720 kb on + strand, within rtcA at 3305.747 kb on + strand, within rtcA at 3305.747 kb on + strand, within rtcA at 3305.748 kb on - strand, within rtcA at 3305.814 kb on + strand, within rtcA at 3305.871 kb on - strand, within rtcA at 3305.871 kb on - strand, within rtcA at 3305.873 kb on + strand, within rtcA at 3306.146 kb on + strand, within rtcA at 3306.183 kb on + strand, within rtcA at 3306.183 kb on + strand, within rtcA at 3306.184 kb on - strand, within rtcA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,303,152 + rtcR OKFHMN_16945 0.55 +0.3 3,303,175 - rtcR OKFHMN_16945 0.57 -1.1 3,303,183 + rtcR OKFHMN_16945 0.57 +0.4 3,303,184 - rtcR OKFHMN_16945 0.57 +0.0 3,303,185 + rtcR OKFHMN_16945 0.57 +1.0 3,303,320 + rtcR OKFHMN_16945 0.66 +2.3 3,303,375 - rtcR OKFHMN_16945 0.69 +1.0 3,303,377 - rtcR OKFHMN_16945 0.69 +1.7 3,303,377 - rtcR OKFHMN_16945 0.69 +0.3 3,303,384 + rtcR OKFHMN_16945 0.70 +0.1 3,303,384 + rtcR OKFHMN_16945 0.70 -0.3 3,303,456 + rtcR OKFHMN_16945 0.74 -0.9 3,303,545 - rtcR OKFHMN_16945 0.80 +0.6 3,303,601 + rtcR OKFHMN_16945 0.83 -0.2 3,303,690 - rtcR OKFHMN_16945 0.89 -0.8 3,303,690 - rtcR OKFHMN_16945 0.89 +2.8 3,303,768 + -0.0 3,303,768 + -1.0 3,303,769 - +0.3 3,303,771 - -0.9 3,303,771 - -1.7 3,303,821 - -2.3 3,303,821 - +0.2 3,303,861 - +0.3 3,303,870 - -0.6 3,303,879 + -0.8 3,303,880 - -2.7 3,303,881 + -0.1 3,303,892 - +0.0 3,303,947 + +0.0 3,304,146 + -1.1 3,304,179 + rtcB OKFHMN_16950 0.10 -1.1 3,304,180 - rtcB OKFHMN_16950 0.10 +0.3 3,304,199 + rtcB OKFHMN_16950 0.12 +0.5 3,304,199 + rtcB OKFHMN_16950 0.12 +0.3 3,304,199 + rtcB OKFHMN_16950 0.12 -0.5 3,304,200 - rtcB OKFHMN_16950 0.12 -0.7 3,304,200 - rtcB OKFHMN_16950 0.12 +0.7 3,304,302 - rtcB OKFHMN_16950 0.20 -1.3 3,304,302 - rtcB OKFHMN_16950 0.20 +0.0 3,304,402 - rtcB OKFHMN_16950 0.28 +0.2 3,304,516 - rtcB OKFHMN_16950 0.38 -1.0 3,304,516 - rtcB OKFHMN_16950 0.38 -0.1 3,304,516 - rtcB OKFHMN_16950 0.38 -0.0 3,304,522 + rtcB OKFHMN_16950 0.38 -2.0 3,304,522 + rtcB OKFHMN_16950 0.38 -0.3 3,304,523 - rtcB OKFHMN_16950 0.38 +0.1 3,304,523 - rtcB OKFHMN_16950 0.38 -0.2 3,304,523 - rtcB OKFHMN_16950 0.38 +1.4 3,304,523 - rtcB OKFHMN_16950 0.38 +0.2 3,304,523 - rtcB OKFHMN_16950 0.38 +0.6 3,304,523 - rtcB OKFHMN_16950 0.38 -0.3 3,304,523 - rtcB OKFHMN_16950 0.38 -0.9 3,304,524 + rtcB OKFHMN_16950 0.38 +0.4 3,304,524 + rtcB OKFHMN_16950 0.38 +0.1 3,304,525 - rtcB OKFHMN_16950 0.38 +0.2 3,304,599 + rtcB OKFHMN_16950 0.44 -0.7 3,304,645 + rtcB OKFHMN_16950 0.48 -0.1 3,304,720 - rtcB OKFHMN_16950 0.54 +0.3 3,304,735 + rtcB OKFHMN_16950 0.56 -0.7 3,304,900 + rtcB OKFHMN_16950 0.69 +0.3 3,304,900 + rtcB OKFHMN_16950 0.69 -0.0 3,304,937 - rtcB OKFHMN_16950 0.72 +0.3 3,305,092 - rtcB OKFHMN_16950 0.85 +0.4 3,305,191 + +0.4 3,305,191 + +0.2 3,305,192 - -0.0 3,305,199 + +0.5 3,305,200 - -0.1 3,305,200 - -0.1 3,305,241 + -0.1 3,305,241 + +0.7 3,305,245 + -1.1 3,305,245 + -0.3 3,305,245 + +0.8 3,305,246 - -0.6 3,305,253 - +0.8 3,305,253 - +0.3 3,305,270 + -0.1 3,305,270 + +0.4 3,305,278 + -0.0 3,305,382 + +0.8 3,305,383 - -0.6 3,305,577 + rtcA OKFHMN_16955 0.28 +0.7 3,305,720 + rtcA OKFHMN_16955 0.42 +0.3 3,305,747 + rtcA OKFHMN_16955 0.45 +0.2 3,305,747 + rtcA OKFHMN_16955 0.45 -0.8 3,305,748 - rtcA OKFHMN_16955 0.45 +0.1 3,305,814 + rtcA OKFHMN_16955 0.52 -0.3 3,305,871 - rtcA OKFHMN_16955 0.57 -0.5 3,305,871 - rtcA OKFHMN_16955 0.57 -0.3 3,305,873 + rtcA OKFHMN_16955 0.57 -1.3 3,306,146 + rtcA OKFHMN_16955 0.84 +0.8 3,306,183 + rtcA OKFHMN_16955 0.87 -0.9 3,306,183 + rtcA OKFHMN_16955 0.87 +0.0 3,306,184 - rtcA OKFHMN_16955 0.87 +0.8
Or see this region's nucleotide sequence