Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glpG and glpR are separated by 16 nucleotides glpR and rtcR overlap by 19 nucleotides rtcR and rtcB are separated by 187 nucleotides
OKFHMN_16935: glpG - rhomboid family intramembrane serine protease GlpG, at 3,300,681 to 3,301,511
glpG
OKFHMN_16940: glpR - DeoR/GlpR family transcriptional regulator, at 3,301,528 to 3,302,286
glpR
OKFHMN_16945: rtcR - DNA-binding transcriptional regulator RtcR, at 3,302,268 to 3,303,866
rtcR
OKFHMN_16950: rtcB - RNA-splicing ligase RtcB, at 3,304,054 to 3,305,280
rtcB
Position (kb)
3302
3303
3304 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3301.293 kb on + strand, within glpG at 3301.309 kb on - strand, within glpG at 3301.351 kb on - strand, within glpG at 3301.366 kb on + strand, within glpG at 3301.366 kb on + strand, within glpG at 3301.367 kb on - strand, within glpG at 3301.555 kb on - strand at 3301.582 kb on + strand at 3301.582 kb on + strand at 3301.582 kb on + strand at 3301.583 kb on - strand at 3301.583 kb on - strand at 3301.583 kb on - strand at 3301.583 kb on - strand at 3301.633 kb on - strand, within glpR at 3301.737 kb on + strand, within glpR at 3301.905 kb on + strand, within glpR at 3301.959 kb on - strand, within glpR at 3302.112 kb on + strand, within glpR at 3302.182 kb on - strand, within glpR at 3302.182 kb on - strand, within glpR at 3302.201 kb on + strand, within glpR at 3302.202 kb on - strand, within glpR at 3302.266 kb on - strand at 3302.326 kb on + strand at 3302.327 kb on - strand at 3302.454 kb on - strand, within rtcR at 3302.531 kb on + strand, within rtcR at 3302.573 kb on + strand, within rtcR at 3302.746 kb on + strand, within rtcR at 3302.757 kb on - strand, within rtcR at 3302.767 kb on - strand, within rtcR at 3302.827 kb on - strand, within rtcR at 3303.037 kb on - strand, within rtcR at 3303.152 kb on + strand, within rtcR at 3303.175 kb on - strand, within rtcR at 3303.183 kb on + strand, within rtcR at 3303.184 kb on - strand, within rtcR at 3303.185 kb on + strand, within rtcR at 3303.320 kb on + strand, within rtcR at 3303.375 kb on - strand, within rtcR at 3303.377 kb on - strand, within rtcR at 3303.377 kb on - strand, within rtcR at 3303.384 kb on + strand, within rtcR at 3303.384 kb on + strand, within rtcR at 3303.456 kb on + strand, within rtcR at 3303.545 kb on - strand, within rtcR at 3303.601 kb on + strand, within rtcR at 3303.690 kb on - strand, within rtcR at 3303.690 kb on - strand, within rtcR at 3303.768 kb on + strand at 3303.768 kb on + strand at 3303.769 kb on - strand at 3303.771 kb on - strand at 3303.771 kb on - strand at 3303.821 kb on - strand at 3303.821 kb on - strand at 3303.861 kb on - strand at 3303.870 kb on - strand at 3303.879 kb on + strand at 3303.880 kb on - strand at 3303.881 kb on + strand at 3303.892 kb on - strand at 3303.947 kb on + strand at 3304.146 kb on + strand at 3304.179 kb on + strand, within rtcB at 3304.180 kb on - strand, within rtcB at 3304.199 kb on + strand, within rtcB at 3304.199 kb on + strand, within rtcB at 3304.199 kb on + strand, within rtcB at 3304.200 kb on - strand, within rtcB at 3304.200 kb on - strand, within rtcB at 3304.302 kb on - strand, within rtcB at 3304.302 kb on - strand, within rtcB at 3304.402 kb on - strand, within rtcB at 3304.516 kb on - strand, within rtcB at 3304.516 kb on - strand, within rtcB at 3304.516 kb on - strand, within rtcB at 3304.522 kb on + strand, within rtcB at 3304.522 kb on + strand, within rtcB at 3304.523 kb on - strand, within rtcB at 3304.523 kb on - strand, within rtcB at 3304.523 kb on - strand, within rtcB at 3304.523 kb on - strand, within rtcB at 3304.523 kb on - strand, within rtcB at 3304.523 kb on - strand, within rtcB at 3304.523 kb on - strand, within rtcB at 3304.524 kb on + strand, within rtcB at 3304.524 kb on + strand, within rtcB at 3304.525 kb on - strand, within rtcB at 3304.599 kb on + strand, within rtcB at 3304.645 kb on + strand, within rtcB at 3304.720 kb on - strand, within rtcB at 3304.735 kb on + strand, within rtcB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,301,293 + glpG OKFHMN_16935 0.74 -0.1 3,301,309 - glpG OKFHMN_16935 0.76 +0.3 3,301,351 - glpG OKFHMN_16935 0.81 -2.6 3,301,366 + glpG OKFHMN_16935 0.82 +0.2 3,301,366 + glpG OKFHMN_16935 0.82 -0.4 3,301,367 - glpG OKFHMN_16935 0.83 +0.5 3,301,555 - +0.5 3,301,582 + -0.3 3,301,582 + -3.6 3,301,582 + -2.4 3,301,583 - -0.2 3,301,583 - -0.7 3,301,583 - -1.2 3,301,583 - -0.9 3,301,633 - glpR OKFHMN_16940 0.14 -1.4 3,301,737 + glpR OKFHMN_16940 0.28 -1.5 3,301,905 + glpR OKFHMN_16940 0.50 -1.1 3,301,959 - glpR OKFHMN_16940 0.57 +0.4 3,302,112 + glpR OKFHMN_16940 0.77 -0.7 3,302,182 - glpR OKFHMN_16940 0.86 -0.3 3,302,182 - glpR OKFHMN_16940 0.86 -0.3 3,302,201 + glpR OKFHMN_16940 0.89 -1.0 3,302,202 - glpR OKFHMN_16940 0.89 -2.9 3,302,266 - +0.6 3,302,326 + +0.2 3,302,327 - -0.1 3,302,454 - rtcR OKFHMN_16945 0.12 -2.4 3,302,531 + rtcR OKFHMN_16945 0.16 -0.1 3,302,573 + rtcR OKFHMN_16945 0.19 +0.1 3,302,746 + rtcR OKFHMN_16945 0.30 -0.4 3,302,757 - rtcR OKFHMN_16945 0.31 +1.0 3,302,767 - rtcR OKFHMN_16945 0.31 -0.3 3,302,827 - rtcR OKFHMN_16945 0.35 -0.7 3,303,037 - rtcR OKFHMN_16945 0.48 +0.6 3,303,152 + rtcR OKFHMN_16945 0.55 +0.3 3,303,175 - rtcR OKFHMN_16945 0.57 -1.1 3,303,183 + rtcR OKFHMN_16945 0.57 +0.4 3,303,184 - rtcR OKFHMN_16945 0.57 +0.0 3,303,185 + rtcR OKFHMN_16945 0.57 +1.0 3,303,320 + rtcR OKFHMN_16945 0.66 +2.3 3,303,375 - rtcR OKFHMN_16945 0.69 +1.0 3,303,377 - rtcR OKFHMN_16945 0.69 +1.7 3,303,377 - rtcR OKFHMN_16945 0.69 +0.3 3,303,384 + rtcR OKFHMN_16945 0.70 +0.1 3,303,384 + rtcR OKFHMN_16945 0.70 -0.3 3,303,456 + rtcR OKFHMN_16945 0.74 -0.9 3,303,545 - rtcR OKFHMN_16945 0.80 +0.6 3,303,601 + rtcR OKFHMN_16945 0.83 -0.2 3,303,690 - rtcR OKFHMN_16945 0.89 -0.8 3,303,690 - rtcR OKFHMN_16945 0.89 +2.8 3,303,768 + -0.0 3,303,768 + -1.0 3,303,769 - +0.3 3,303,771 - -0.9 3,303,771 - -1.7 3,303,821 - -2.3 3,303,821 - +0.2 3,303,861 - +0.3 3,303,870 - -0.6 3,303,879 + -0.8 3,303,880 - -2.7 3,303,881 + -0.1 3,303,892 - +0.0 3,303,947 + +0.0 3,304,146 + -1.1 3,304,179 + rtcB OKFHMN_16950 0.10 -1.1 3,304,180 - rtcB OKFHMN_16950 0.10 +0.3 3,304,199 + rtcB OKFHMN_16950 0.12 +0.5 3,304,199 + rtcB OKFHMN_16950 0.12 +0.3 3,304,199 + rtcB OKFHMN_16950 0.12 -0.5 3,304,200 - rtcB OKFHMN_16950 0.12 -0.7 3,304,200 - rtcB OKFHMN_16950 0.12 +0.7 3,304,302 - rtcB OKFHMN_16950 0.20 -1.3 3,304,302 - rtcB OKFHMN_16950 0.20 +0.0 3,304,402 - rtcB OKFHMN_16950 0.28 +0.2 3,304,516 - rtcB OKFHMN_16950 0.38 -1.0 3,304,516 - rtcB OKFHMN_16950 0.38 -0.1 3,304,516 - rtcB OKFHMN_16950 0.38 -0.0 3,304,522 + rtcB OKFHMN_16950 0.38 -2.0 3,304,522 + rtcB OKFHMN_16950 0.38 -0.3 3,304,523 - rtcB OKFHMN_16950 0.38 +0.1 3,304,523 - rtcB OKFHMN_16950 0.38 -0.2 3,304,523 - rtcB OKFHMN_16950 0.38 +1.4 3,304,523 - rtcB OKFHMN_16950 0.38 +0.2 3,304,523 - rtcB OKFHMN_16950 0.38 +0.6 3,304,523 - rtcB OKFHMN_16950 0.38 -0.3 3,304,523 - rtcB OKFHMN_16950 0.38 -0.9 3,304,524 + rtcB OKFHMN_16950 0.38 +0.4 3,304,524 + rtcB OKFHMN_16950 0.38 +0.1 3,304,525 - rtcB OKFHMN_16950 0.38 +0.2 3,304,599 + rtcB OKFHMN_16950 0.44 -0.7 3,304,645 + rtcB OKFHMN_16950 0.48 -0.1 3,304,720 - rtcB OKFHMN_16950 0.54 +0.3 3,304,735 + rtcB OKFHMN_16950 0.56 -0.7
Or see this region's nucleotide sequence