Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glpD and glpE are separated by 189 nucleotides glpE and glpG are separated by 44 nucleotides glpG and glpR are separated by 16 nucleotides glpR and rtcR overlap by 19 nucleotides
OKFHMN_16925: glpD - glycerol-3-phosphate dehydrogenase, at 3,298,615 to 3,300,120
glpD
OKFHMN_16930: glpE - thiosulfate sulfurtransferase GlpE, at 3,300,310 to 3,300,636
glpE
OKFHMN_16935: glpG - rhomboid family intramembrane serine protease GlpG, at 3,300,681 to 3,301,511
glpG
OKFHMN_16940: glpR - DeoR/GlpR family transcriptional regulator, at 3,301,528 to 3,302,286
glpR
OKFHMN_16945: rtcR - DNA-binding transcriptional regulator RtcR, at 3,302,268 to 3,303,866
rtcR
Position (kb)
3300
3301
3302 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3299.798 kb on + strand, within glpD at 3299.799 kb on - strand, within glpD at 3299.803 kb on - strand, within glpD at 3300.094 kb on + strand at 3300.094 kb on + strand at 3300.152 kb on - strand at 3300.224 kb on - strand at 3300.227 kb on + strand at 3300.228 kb on - strand at 3300.228 kb on - strand at 3300.228 kb on - strand at 3300.228 kb on - strand at 3300.229 kb on + strand at 3300.230 kb on - strand at 3300.230 kb on - strand at 3300.244 kb on + strand at 3300.244 kb on + strand at 3300.245 kb on - strand at 3300.262 kb on - strand at 3300.327 kb on + strand at 3300.328 kb on - strand at 3300.328 kb on - strand at 3300.330 kb on + strand at 3300.468 kb on + strand, within glpE at 3300.468 kb on + strand, within glpE at 3300.469 kb on - strand, within glpE at 3300.469 kb on - strand, within glpE at 3300.472 kb on - strand, within glpE at 3300.506 kb on - strand, within glpE at 3300.539 kb on - strand, within glpE at 3300.539 kb on - strand, within glpE at 3300.556 kb on + strand, within glpE at 3300.568 kb on + strand, within glpE at 3300.568 kb on + strand, within glpE at 3300.568 kb on + strand, within glpE at 3300.568 kb on + strand, within glpE at 3300.569 kb on - strand, within glpE at 3300.570 kb on + strand, within glpE at 3300.571 kb on - strand, within glpE at 3300.642 kb on + strand at 3300.643 kb on - strand at 3300.735 kb on + strand at 3300.735 kb on + strand at 3300.736 kb on - strand at 3300.777 kb on - strand, within glpG at 3301.004 kb on + strand, within glpG at 3301.042 kb on + strand, within glpG at 3301.092 kb on + strand, within glpG at 3301.093 kb on - strand, within glpG at 3301.202 kb on - strand, within glpG at 3301.211 kb on + strand, within glpG at 3301.212 kb on - strand, within glpG at 3301.217 kb on + strand, within glpG at 3301.239 kb on + strand, within glpG at 3301.240 kb on - strand, within glpG at 3301.240 kb on - strand, within glpG at 3301.293 kb on + strand, within glpG at 3301.309 kb on - strand, within glpG at 3301.351 kb on - strand, within glpG at 3301.366 kb on + strand, within glpG at 3301.366 kb on + strand, within glpG at 3301.367 kb on - strand, within glpG at 3301.555 kb on - strand at 3301.582 kb on + strand at 3301.582 kb on + strand at 3301.582 kb on + strand at 3301.583 kb on - strand at 3301.583 kb on - strand at 3301.583 kb on - strand at 3301.583 kb on - strand at 3301.633 kb on - strand, within glpR at 3301.737 kb on + strand, within glpR at 3301.905 kb on + strand, within glpR at 3301.959 kb on - strand, within glpR at 3302.112 kb on + strand, within glpR at 3302.182 kb on - strand, within glpR at 3302.182 kb on - strand, within glpR at 3302.201 kb on + strand, within glpR at 3302.202 kb on - strand, within glpR at 3302.266 kb on - strand at 3302.326 kb on + strand at 3302.327 kb on - strand at 3302.454 kb on - strand, within rtcR
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,299,798 + glpD OKFHMN_16925 0.79 -0.7 3,299,799 - glpD OKFHMN_16925 0.79 -1.0 3,299,803 - glpD OKFHMN_16925 0.79 -3.0 3,300,094 + -0.5 3,300,094 + -0.8 3,300,152 - +0.2 3,300,224 - -0.3 3,300,227 + -0.1 3,300,228 - -0.3 3,300,228 - -0.1 3,300,228 - +1.3 3,300,228 - -0.2 3,300,229 + +0.1 3,300,230 - +1.0 3,300,230 - +0.7 3,300,244 + +0.0 3,300,244 + -1.6 3,300,245 - -0.2 3,300,262 - -0.6 3,300,327 + -0.4 3,300,328 - -1.3 3,300,328 - -0.6 3,300,330 + +0.3 3,300,468 + glpE OKFHMN_16930 0.48 -0.1 3,300,468 + glpE OKFHMN_16930 0.48 +0.8 3,300,469 - glpE OKFHMN_16930 0.49 -0.2 3,300,469 - glpE OKFHMN_16930 0.49 -0.5 3,300,472 - glpE OKFHMN_16930 0.50 -1.4 3,300,506 - glpE OKFHMN_16930 0.60 -0.7 3,300,539 - glpE OKFHMN_16930 0.70 -1.7 3,300,539 - glpE OKFHMN_16930 0.70 -0.5 3,300,556 + glpE OKFHMN_16930 0.75 +0.3 3,300,568 + glpE OKFHMN_16930 0.79 +0.5 3,300,568 + glpE OKFHMN_16930 0.79 -0.5 3,300,568 + glpE OKFHMN_16930 0.79 -0.2 3,300,568 + glpE OKFHMN_16930 0.79 +1.0 3,300,569 - glpE OKFHMN_16930 0.79 -0.8 3,300,570 + glpE OKFHMN_16930 0.80 -0.2 3,300,571 - glpE OKFHMN_16930 0.80 +0.3 3,300,642 + +0.4 3,300,643 - -1.7 3,300,735 + -1.0 3,300,735 + -0.2 3,300,736 - +0.7 3,300,777 - glpG OKFHMN_16935 0.12 -1.7 3,301,004 + glpG OKFHMN_16935 0.39 -0.0 3,301,042 + glpG OKFHMN_16935 0.43 +0.3 3,301,092 + glpG OKFHMN_16935 0.49 +0.2 3,301,093 - glpG OKFHMN_16935 0.50 -0.1 3,301,202 - glpG OKFHMN_16935 0.63 -0.2 3,301,211 + glpG OKFHMN_16935 0.64 +0.4 3,301,212 - glpG OKFHMN_16935 0.64 +0.2 3,301,217 + glpG OKFHMN_16935 0.65 +0.6 3,301,239 + glpG OKFHMN_16935 0.67 -0.5 3,301,240 - glpG OKFHMN_16935 0.67 -1.1 3,301,240 - glpG OKFHMN_16935 0.67 -1.0 3,301,293 + glpG OKFHMN_16935 0.74 -0.1 3,301,309 - glpG OKFHMN_16935 0.76 +0.3 3,301,351 - glpG OKFHMN_16935 0.81 -2.6 3,301,366 + glpG OKFHMN_16935 0.82 +0.2 3,301,366 + glpG OKFHMN_16935 0.82 -0.4 3,301,367 - glpG OKFHMN_16935 0.83 +0.5 3,301,555 - +0.5 3,301,582 + -0.3 3,301,582 + -3.6 3,301,582 + -2.4 3,301,583 - -0.2 3,301,583 - -0.7 3,301,583 - -1.2 3,301,583 - -0.9 3,301,633 - glpR OKFHMN_16940 0.14 -1.4 3,301,737 + glpR OKFHMN_16940 0.28 -1.5 3,301,905 + glpR OKFHMN_16940 0.50 -1.1 3,301,959 - glpR OKFHMN_16940 0.57 +0.4 3,302,112 + glpR OKFHMN_16940 0.77 -0.7 3,302,182 - glpR OKFHMN_16940 0.86 -0.3 3,302,182 - glpR OKFHMN_16940 0.86 -0.3 3,302,201 + glpR OKFHMN_16940 0.89 -1.0 3,302,202 - glpR OKFHMN_16940 0.89 -2.9 3,302,266 - +0.6 3,302,326 + +0.2 3,302,327 - -0.1 3,302,454 - rtcR OKFHMN_16945 0.12 -2.4
Or see this region's nucleotide sequence