Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nikC and nikB overlap by 4 nucleotides nikB and nikA overlap by 1 nucleotides nikA and acpT are separated by 110 nucleotides acpT and fabB are separated by 1 nucleotides
OKFHMN_16540: nikC - nickel ABC transporter permease subunit NikC, at 3,223,464 to 3,224,297
nikC
OKFHMN_16545: nikB - nickel ABC transporter permease subunit NikB, at 3,224,294 to 3,225,238
nikB
OKFHMN_16550: nikA - nickel ABC transporter substrate-binding protein, at 3,225,238 to 3,226,812
nikA
OKFHMN_16555: acpT - 4'-phosphopantetheinyl transferase AcpT, at 3,226,923 to 3,227,510
acpT
OKFHMN_16560: fabB - beta-ketoacyl-ACP synthase, at 3,227,512 to 3,228,741
fabB
Position (kb)
3225
3226
3227 Strain fitness (log2 ratio)
-2
-1
0
1 at 3224.365 kb on + strand at 3224.366 kb on - strand at 3224.399 kb on - strand, within nikB at 3224.399 kb on - strand, within nikB at 3224.683 kb on + strand, within nikB at 3224.730 kb on + strand, within nikB at 3224.907 kb on - strand, within nikB at 3224.910 kb on - strand, within nikB at 3225.045 kb on + strand, within nikB at 3225.045 kb on + strand, within nikB at 3225.224 kb on + strand at 3225.225 kb on - strand at 3225.229 kb on - strand at 3225.240 kb on - strand at 3225.341 kb on + strand at 3225.342 kb on - strand at 3225.342 kb on - strand at 3225.342 kb on - strand at 3225.342 kb on - strand at 3225.356 kb on + strand at 3225.395 kb on + strand at 3225.542 kb on + strand, within nikA at 3225.543 kb on - strand, within nikA at 3225.602 kb on + strand, within nikA at 3225.607 kb on - strand, within nikA at 3225.607 kb on - strand, within nikA at 3225.658 kb on - strand, within nikA at 3225.818 kb on + strand, within nikA at 3225.897 kb on - strand, within nikA at 3225.939 kb on + strand, within nikA at 3225.939 kb on + strand, within nikA at 3225.987 kb on + strand, within nikA at 3226.034 kb on + strand, within nikA at 3226.035 kb on - strand, within nikA at 3226.035 kb on - strand, within nikA at 3226.066 kb on - strand, within nikA at 3226.079 kb on - strand, within nikA at 3226.173 kb on + strand, within nikA at 3226.223 kb on + strand, within nikA at 3226.368 kb on - strand, within nikA at 3226.370 kb on + strand, within nikA at 3226.370 kb on + strand, within nikA at 3226.405 kb on - strand, within nikA at 3226.567 kb on - strand, within nikA at 3226.646 kb on + strand, within nikA at 3226.758 kb on + strand at 3226.786 kb on + strand at 3226.786 kb on + strand at 3226.829 kb on + strand at 3226.834 kb on + strand at 3226.847 kb on + strand at 3226.848 kb on - strand at 3226.852 kb on - strand at 3226.852 kb on - strand at 3226.863 kb on - strand at 3226.899 kb on + strand at 3226.900 kb on - strand at 3226.900 kb on - strand at 3226.900 kb on - strand at 3227.041 kb on + strand, within acpT at 3227.041 kb on + strand, within acpT at 3227.041 kb on + strand, within acpT at 3227.042 kb on - strand, within acpT at 3227.042 kb on - strand, within acpT at 3227.298 kb on + strand, within acpT at 3227.298 kb on + strand, within acpT at 3227.353 kb on + strand, within acpT at 3227.353 kb on + strand, within acpT at 3227.354 kb on - strand, within acpT at 3227.354 kb on - strand, within acpT at 3227.499 kb on + strand at 3227.510 kb on + strand at 3227.510 kb on + strand at 3227.511 kb on - strand at 3227.526 kb on + strand at 3227.541 kb on + strand at 3227.541 kb on + strand at 3227.542 kb on - strand at 3227.542 kb on - strand at 3227.542 kb on - strand at 3227.542 kb on - strand at 3227.542 kb on - strand at 3227.542 kb on - strand at 3227.569 kb on - strand at 3227.733 kb on + strand, within fabB at 3227.734 kb on - strand, within fabB at 3227.734 kb on - strand, within fabB at 3227.780 kb on + strand, within fabB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,224,365 + -0.8 3,224,366 - -0.7 3,224,399 - nikB OKFHMN_16545 0.11 +0.3 3,224,399 - nikB OKFHMN_16545 0.11 +0.3 3,224,683 + nikB OKFHMN_16545 0.41 +0.1 3,224,730 + nikB OKFHMN_16545 0.46 -0.1 3,224,907 - nikB OKFHMN_16545 0.65 -0.3 3,224,910 - nikB OKFHMN_16545 0.65 +0.7 3,225,045 + nikB OKFHMN_16545 0.79 +0.3 3,225,045 + nikB OKFHMN_16545 0.79 -0.5 3,225,224 + -0.1 3,225,225 - +0.1 3,225,229 - -2.0 3,225,240 - -0.1 3,225,341 + -0.0 3,225,342 - +0.4 3,225,342 - +1.1 3,225,342 - +0.3 3,225,342 - +0.3 3,225,356 + +0.6 3,225,395 + -0.2 3,225,542 + nikA OKFHMN_16550 0.19 -0.1 3,225,543 - nikA OKFHMN_16550 0.19 +0.1 3,225,602 + nikA OKFHMN_16550 0.23 +1.2 3,225,607 - nikA OKFHMN_16550 0.23 -0.2 3,225,607 - nikA OKFHMN_16550 0.23 +0.7 3,225,658 - nikA OKFHMN_16550 0.27 -2.0 3,225,818 + nikA OKFHMN_16550 0.37 +0.2 3,225,897 - nikA OKFHMN_16550 0.42 +0.7 3,225,939 + nikA OKFHMN_16550 0.45 +0.7 3,225,939 + nikA OKFHMN_16550 0.45 +1.0 3,225,987 + nikA OKFHMN_16550 0.48 +0.3 3,226,034 + nikA OKFHMN_16550 0.51 -1.5 3,226,035 - nikA OKFHMN_16550 0.51 +0.1 3,226,035 - nikA OKFHMN_16550 0.51 -0.2 3,226,066 - nikA OKFHMN_16550 0.53 +0.3 3,226,079 - nikA OKFHMN_16550 0.53 +1.2 3,226,173 + nikA OKFHMN_16550 0.59 -1.9 3,226,223 + nikA OKFHMN_16550 0.63 +0.4 3,226,368 - nikA OKFHMN_16550 0.72 +0.6 3,226,370 + nikA OKFHMN_16550 0.72 -0.2 3,226,370 + nikA OKFHMN_16550 0.72 -0.5 3,226,405 - nikA OKFHMN_16550 0.74 +0.9 3,226,567 - nikA OKFHMN_16550 0.84 -1.0 3,226,646 + nikA OKFHMN_16550 0.89 -0.8 3,226,758 + +0.6 3,226,786 + +0.7 3,226,786 + +0.3 3,226,829 + +0.5 3,226,834 + -0.1 3,226,847 + +0.2 3,226,848 - -1.0 3,226,852 - -0.4 3,226,852 - -0.8 3,226,863 - -2.1 3,226,899 + -0.4 3,226,900 - -0.2 3,226,900 - -0.2 3,226,900 - -0.0 3,227,041 + acpT OKFHMN_16555 0.20 +0.1 3,227,041 + acpT OKFHMN_16555 0.20 +0.2 3,227,041 + acpT OKFHMN_16555 0.20 +1.4 3,227,042 - acpT OKFHMN_16555 0.20 +1.4 3,227,042 - acpT OKFHMN_16555 0.20 -0.2 3,227,298 + acpT OKFHMN_16555 0.64 -0.2 3,227,298 + acpT OKFHMN_16555 0.64 +0.1 3,227,353 + acpT OKFHMN_16555 0.73 -1.4 3,227,353 + acpT OKFHMN_16555 0.73 +1.2 3,227,354 - acpT OKFHMN_16555 0.73 +0.4 3,227,354 - acpT OKFHMN_16555 0.73 +0.2 3,227,499 + +1.2 3,227,510 + +1.6 3,227,510 + -0.2 3,227,511 - +0.4 3,227,526 + -0.4 3,227,541 + +1.6 3,227,541 + +1.5 3,227,542 - -0.1 3,227,542 - +0.2 3,227,542 - +0.3 3,227,542 - +0.5 3,227,542 - -1.2 3,227,542 - -0.6 3,227,569 - -1.1 3,227,733 + fabB OKFHMN_16560 0.18 -2.7 3,227,734 - fabB OKFHMN_16560 0.18 +0.1 3,227,734 - fabB OKFHMN_16560 0.18 -1.5 3,227,780 + fabB OKFHMN_16560 0.22 +0.8
Or see this region's nucleotide sequence