Strain Fitness in Escherichia coli ECRC100 around OKFHMN_14230

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nt16S_rrna and hemG are separated by 380 nucleotideshemG and trkH are separated by 11 nucleotidestrkH and yigZ are separated by 38 nucleotidesyigZ and pepQ overlap by 1 nucleotides OKFHMN_14220: 16S_rrna - 16S ribosomal RNA, at 2,741,847 to 2,743,388 _rrna OKFHMN_14225: hemG - menaquinone-dependent protoporphyrinogen IX dehydrogenase, at 2,743,769 to 2,744,314 hemG OKFHMN_14230: trkH - Trk system potassium transporter TrkH, at 2,744,326 to 2,745,777 trkH OKFHMN_14235: yigZ - IMPACT family protein, at 2,745,816 to 2,746,430 yigZ OKFHMN_14240: pepQ - Xaa-Pro dipeptidase, at 2,746,430 to 2,747,761 pepQ Position (kb) 2744 2745 2746Strain fitness (log2 ratio) -2 -1 0 1at 2743.771 kb on - strandat 2744.316 kb on + strandat 2744.316 kb on + strandat 2744.317 kb on - strandat 2744.317 kb on - strandat 2744.317 kb on - strandat 2744.319 kb on + strandat 2744.319 kb on + strandat 2744.319 kb on + strandat 2744.320 kb on - strandat 2744.320 kb on - strandat 2744.320 kb on - strandat 2745.057 kb on + strand, within trkHat 2745.793 kb on - strandat 2745.793 kb on - strandat 2745.793 kb on - strandat 2745.944 kb on + strand, within yigZat 2745.945 kb on - strand, within yigZat 2745.996 kb on - strand, within yigZat 2745.997 kb on + strand, within yigZat 2745.997 kb on + strand, within yigZat 2745.997 kb on + strand, within yigZat 2745.998 kb on - strand, within yigZat 2746.013 kb on + strand, within yigZat 2746.014 kb on - strand, within yigZat 2746.016 kb on - strand, within yigZat 2746.016 kb on - strand, within yigZat 2746.113 kb on + strand, within yigZat 2746.126 kb on + strand, within yigZat 2746.127 kb on - strand, within yigZat 2746.171 kb on - strand, within yigZat 2746.347 kb on - strand, within yigZat 2746.347 kb on - strand, within yigZat 2746.417 kb on - strandat 2746.599 kb on + strand, within pepQat 2746.599 kb on + strand, within pepQat 2746.599 kb on + strand, within pepQat 2746.600 kb on - strand, within pepQat 2746.600 kb on - strand, within pepQat 2746.600 kb on - strand, within pepQat 2746.619 kb on + strand, within pepQat 2746.620 kb on - strand, within pepQat 2746.642 kb on + strand, within pepQ

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,743,771 - -0.3
2,744,316 + -0.1
2,744,316 + -0.6
2,744,317 - +0.2
2,744,317 - +0.2
2,744,317 - +0.5
2,744,319 + -0.5
2,744,319 + -0.3
2,744,319 + +1.0
2,744,320 - +0.7
2,744,320 - +0.8
2,744,320 - -0.4
2,745,057 + trkH OKFHMN_14230 0.50 -1.3
2,745,793 - +0.2
2,745,793 - -0.1
2,745,793 - +0.8
2,745,944 + yigZ OKFHMN_14235 0.21 +0.2
2,745,945 - yigZ OKFHMN_14235 0.21 +0.2
2,745,996 - yigZ OKFHMN_14235 0.29 +0.3
2,745,997 + yigZ OKFHMN_14235 0.29 +0.3
2,745,997 + yigZ OKFHMN_14235 0.29 -0.2
2,745,997 + yigZ OKFHMN_14235 0.29 -2.1
2,745,998 - yigZ OKFHMN_14235 0.30 -0.0
2,746,013 + yigZ OKFHMN_14235 0.32 +0.6
2,746,014 - yigZ OKFHMN_14235 0.32 -0.1
2,746,016 - yigZ OKFHMN_14235 0.33 +0.3
2,746,016 - yigZ OKFHMN_14235 0.33 -1.9
2,746,113 + yigZ OKFHMN_14235 0.48 -0.0
2,746,126 + yigZ OKFHMN_14235 0.50 -0.8
2,746,127 - yigZ OKFHMN_14235 0.51 +0.9
2,746,171 - yigZ OKFHMN_14235 0.58 -0.4
2,746,347 - yigZ OKFHMN_14235 0.86 +0.4
2,746,347 - yigZ OKFHMN_14235 0.86 +1.3
2,746,417 - +0.9
2,746,599 + pepQ OKFHMN_14240 0.13 +0.4
2,746,599 + pepQ OKFHMN_14240 0.13 -0.3
2,746,599 + pepQ OKFHMN_14240 0.13 +0.5
2,746,600 - pepQ OKFHMN_14240 0.13 +0.1
2,746,600 - pepQ OKFHMN_14240 0.13 +0.0
2,746,600 - pepQ OKFHMN_14240 0.13 +0.6
2,746,619 + pepQ OKFHMN_14240 0.14 +0.2
2,746,620 - pepQ OKFHMN_14240 0.14 +0.5
2,746,642 + pepQ OKFHMN_14240 0.16 +0.0

Or see this region's nucleotide sequence