Strain Fitness in Escherichia coli ECRC100 around OKFHMN_13625

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpflD and frwB are separated by 50 nucleotidesfrwB and frwC are separated by 14 nucleotides OKFHMN_13620: pflD - formate C-acetyltransferase, at 2,621,868 to 2,624,165 pflD OKFHMN_13625: frwB - PTS fructose-like transporter subunit IIB, at 2,624,216 to 2,624,536 frwB OKFHMN_13630: frwC - PTS fructose transporter subunit EIIC, at 2,624,551 to 2,625,630 frwC Position (kb) 2624 2625Strain fitness (log2 ratio) -3 -2 -1 0 1at 2623.327 kb on + strand, within pflDat 2623.340 kb on + strand, within pflDat 2623.340 kb on + strand, within pflDat 2623.340 kb on + strand, within pflDat 2623.340 kb on + strand, within pflDat 2623.340 kb on + strand, within pflDat 2623.340 kb on + strand, within pflDat 2623.340 kb on + strand, within pflDat 2623.341 kb on - strand, within pflDat 2623.341 kb on - strand, within pflDat 2623.341 kb on - strand, within pflDat 2623.341 kb on - strand, within pflDat 2623.343 kb on - strand, within pflDat 2623.408 kb on + strand, within pflDat 2623.408 kb on + strand, within pflDat 2623.417 kb on - strand, within pflDat 2623.691 kb on + strand, within pflDat 2623.691 kb on + strand, within pflDat 2623.691 kb on + strand, within pflDat 2623.694 kb on + strand, within pflDat 2623.838 kb on + strand, within pflDat 2623.838 kb on + strand, within pflDat 2623.838 kb on + strand, within pflDat 2623.839 kb on - strand, within pflDat 2623.839 kb on - strand, within pflDat 2623.845 kb on - strand, within pflDat 2623.859 kb on + strand, within pflDat 2624.020 kb on + strandat 2624.020 kb on + strandat 2624.020 kb on + strandat 2624.021 kb on - strandat 2624.021 kb on - strandat 2624.046 kb on + strandat 2624.086 kb on + strandat 2624.086 kb on + strandat 2624.087 kb on - strandat 2624.154 kb on - strandat 2624.154 kb on - strandat 2624.217 kb on + strandat 2624.254 kb on + strand, within frwBat 2624.348 kb on - strand, within frwBat 2624.482 kb on + strand, within frwBat 2624.482 kb on + strand, within frwBat 2624.483 kb on - strand, within frwBat 2624.483 kb on - strand, within frwBat 2624.483 kb on - strand, within frwBat 2624.484 kb on + strand, within frwBat 2624.484 kb on + strand, within frwBat 2624.484 kb on + strand, within frwBat 2624.484 kb on + strand, within frwBat 2624.484 kb on + strand, within frwBat 2624.484 kb on + strand, within frwBat 2624.484 kb on + strand, within frwBat 2624.484 kb on + strand, within frwBat 2624.484 kb on + strand, within frwBat 2624.484 kb on + strand, within frwBat 2624.484 kb on + strand, within frwBat 2624.485 kb on - strand, within frwBat 2624.485 kb on - strand, within frwBat 2624.485 kb on - strand, within frwBat 2624.485 kb on - strand, within frwBat 2624.485 kb on - strand, within frwBat 2624.485 kb on - strand, within frwBat 2624.485 kb on - strand, within frwBat 2624.485 kb on - strand, within frwBat 2624.485 kb on - strand, within frwBat 2624.485 kb on - strand, within frwBat 2624.485 kb on - strand, within frwBat 2624.485 kb on - strand, within frwBat 2624.485 kb on - strand, within frwBat 2624.485 kb on - strand, within frwBat 2624.488 kb on + strand, within frwBat 2624.489 kb on - strand, within frwBat 2624.489 kb on - strand, within frwBat 2624.552 kb on + strandat 2624.552 kb on + strandat 2624.599 kb on - strandat 2624.682 kb on + strand, within frwCat 2624.682 kb on + strand, within frwCat 2624.684 kb on + strand, within frwCat 2624.695 kb on - strand, within frwCat 2624.767 kb on + strand, within frwCat 2624.767 kb on + strand, within frwCat 2624.768 kb on - strand, within frwCat 2624.768 kb on - strand, within frwCat 2624.768 kb on - strand, within frwCat 2624.807 kb on - strand, within frwCat 2624.807 kb on - strand, within frwCat 2624.807 kb on - strand, within frwCat 2624.813 kb on - strand, within frwCat 2624.991 kb on + strand, within frwCat 2624.992 kb on - strand, within frwCat 2624.994 kb on - strand, within frwCat 2624.994 kb on - strand, within frwCat 2624.994 kb on - strand, within frwCat 2625.096 kb on + strand, within frwCat 2625.157 kb on + strand, within frwCat 2625.157 kb on + strand, within frwCat 2625.253 kb on - strand, within frwCat 2625.275 kb on + strand, within frwCat 2625.512 kb on + strand, within frwC

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,623,327 + pflD OKFHMN_13620 0.63 -0.8
2,623,340 + pflD OKFHMN_13620 0.64 -0.5
2,623,340 + pflD OKFHMN_13620 0.64 +0.6
2,623,340 + pflD OKFHMN_13620 0.64 +0.2
2,623,340 + pflD OKFHMN_13620 0.64 -0.5
2,623,340 + pflD OKFHMN_13620 0.64 +0.8
2,623,340 + pflD OKFHMN_13620 0.64 -0.6
2,623,340 + pflD OKFHMN_13620 0.64 +0.2
2,623,341 - pflD OKFHMN_13620 0.64 -0.4
2,623,341 - pflD OKFHMN_13620 0.64 -0.4
2,623,341 - pflD OKFHMN_13620 0.64 +0.5
2,623,341 - pflD OKFHMN_13620 0.64 +0.4
2,623,343 - pflD OKFHMN_13620 0.64 -0.0
2,623,408 + pflD OKFHMN_13620 0.67 +0.6
2,623,408 + pflD OKFHMN_13620 0.67 -1.4
2,623,417 - pflD OKFHMN_13620 0.67 -0.0
2,623,691 + pflD OKFHMN_13620 0.79 -0.0
2,623,691 + pflD OKFHMN_13620 0.79 -0.7
2,623,691 + pflD OKFHMN_13620 0.79 +0.5
2,623,694 + pflD OKFHMN_13620 0.79 -1.0
2,623,838 + pflD OKFHMN_13620 0.86 +0.0
2,623,838 + pflD OKFHMN_13620 0.86 -0.2
2,623,838 + pflD OKFHMN_13620 0.86 -2.4
2,623,839 - pflD OKFHMN_13620 0.86 -1.8
2,623,839 - pflD OKFHMN_13620 0.86 +0.1
2,623,845 - pflD OKFHMN_13620 0.86 -1.5
2,623,859 + pflD OKFHMN_13620 0.87 -0.5
2,624,020 + -2.1
2,624,020 + +0.3
2,624,020 + -0.7
2,624,021 - -0.7
2,624,021 - +0.7
2,624,046 + -0.1
2,624,086 + -0.2
2,624,086 + -0.2
2,624,087 - +0.3
2,624,154 - -0.5
2,624,154 - +0.7
2,624,217 + -0.3
2,624,254 + frwB OKFHMN_13625 0.12 +0.4
2,624,348 - frwB OKFHMN_13625 0.41 +0.0
2,624,482 + frwB OKFHMN_13625 0.83 -0.1
2,624,482 + frwB OKFHMN_13625 0.83 +0.1
2,624,483 - frwB OKFHMN_13625 0.83 -1.0
2,624,483 - frwB OKFHMN_13625 0.83 +0.4
2,624,483 - frwB OKFHMN_13625 0.83 -0.4
2,624,484 + frwB OKFHMN_13625 0.83 +0.3
2,624,484 + frwB OKFHMN_13625 0.83 +0.2
2,624,484 + frwB OKFHMN_13625 0.83 +0.4
2,624,484 + frwB OKFHMN_13625 0.83 +0.1
2,624,484 + frwB OKFHMN_13625 0.83 +0.5
2,624,484 + frwB OKFHMN_13625 0.83 -1.6
2,624,484 + frwB OKFHMN_13625 0.83 +0.4
2,624,484 + frwB OKFHMN_13625 0.83 +0.0
2,624,484 + frwB OKFHMN_13625 0.83 -0.5
2,624,484 + frwB OKFHMN_13625 0.83 +1.3
2,624,484 + frwB OKFHMN_13625 0.83 +0.1
2,624,485 - frwB OKFHMN_13625 0.84 +0.2
2,624,485 - frwB OKFHMN_13625 0.84 +1.2
2,624,485 - frwB OKFHMN_13625 0.84 -0.4
2,624,485 - frwB OKFHMN_13625 0.84 -2.4
2,624,485 - frwB OKFHMN_13625 0.84 -0.0
2,624,485 - frwB OKFHMN_13625 0.84 -0.1
2,624,485 - frwB OKFHMN_13625 0.84 +0.3
2,624,485 - frwB OKFHMN_13625 0.84 +0.2
2,624,485 - frwB OKFHMN_13625 0.84 -0.3
2,624,485 - frwB OKFHMN_13625 0.84 +0.4
2,624,485 - frwB OKFHMN_13625 0.84 +0.4
2,624,485 - frwB OKFHMN_13625 0.84 +0.3
2,624,485 - frwB OKFHMN_13625 0.84 +0.5
2,624,485 - frwB OKFHMN_13625 0.84 +0.5
2,624,488 + frwB OKFHMN_13625 0.85 +0.9
2,624,489 - frwB OKFHMN_13625 0.85 +0.4
2,624,489 - frwB OKFHMN_13625 0.85 +0.5
2,624,552 + -1.0
2,624,552 + -0.7
2,624,599 - -0.7
2,624,682 + frwC OKFHMN_13630 0.12 +1.6
2,624,682 + frwC OKFHMN_13630 0.12 +0.7
2,624,684 + frwC OKFHMN_13630 0.12 +0.3
2,624,695 - frwC OKFHMN_13630 0.13 +0.2
2,624,767 + frwC OKFHMN_13630 0.20 -0.2
2,624,767 + frwC OKFHMN_13630 0.20 +1.4
2,624,768 - frwC OKFHMN_13630 0.20 +0.0
2,624,768 - frwC OKFHMN_13630 0.20 +0.9
2,624,768 - frwC OKFHMN_13630 0.20 -0.1
2,624,807 - frwC OKFHMN_13630 0.24 +0.2
2,624,807 - frwC OKFHMN_13630 0.24 +0.6
2,624,807 - frwC OKFHMN_13630 0.24 +0.1
2,624,813 - frwC OKFHMN_13630 0.24 +0.2
2,624,991 + frwC OKFHMN_13630 0.41 +0.0
2,624,992 - frwC OKFHMN_13630 0.41 -1.0
2,624,994 - frwC OKFHMN_13630 0.41 -0.0
2,624,994 - frwC OKFHMN_13630 0.41 -0.1
2,624,994 - frwC OKFHMN_13630 0.41 -0.2
2,625,096 + frwC OKFHMN_13630 0.50 -0.7
2,625,157 + frwC OKFHMN_13630 0.56 +0.1
2,625,157 + frwC OKFHMN_13630 0.56 -0.3
2,625,253 - frwC OKFHMN_13630 0.65 -0.1
2,625,275 + frwC OKFHMN_13630 0.67 -1.3
2,625,512 + frwC OKFHMN_13630 0.89 -3.6

Or see this region's nucleotide sequence