Strain Fitness in Escherichia coli ECRC100 around OKFHMN_13430

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrpoB_R687H and rplL are separated by 319 nucleotidesrplL and rplJ are separated by 66 nucleotidesrplJ and rplA are separated by 291 nucleotides OKFHMN_13420: rpoB_R687H - DNA-directed RNA polymerase subunit beta RpoB, at 2,580,873 to 2,584,901 _R687H OKFHMN_13430: rplL - 50S ribosomal protein L7/L12, at 2,585,221 to 2,585,586 rplL OKFHMN_13435: rplJ - 50S ribosomal protein L10, at 2,585,653 to 2,586,150 rplJ OKFHMN_13440: rplA - 50S ribosomal protein L1, at 2,586,442 to 2,587,146 rplA Position (kb) 2585 2586Strain fitness (log2 ratio) -2 -1 0 1at 2585.144 kb on - strandat 2586.449 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,585,144 - +1.2
2,586,449 - -2.4

Or see this region's nucleotide sequence