Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hupA and yjaG are separated by 186 nucleotides yjaG and nfi are separated by 42 nucleotides nfi and hemE are separated by 9 nucleotides
OKFHMN_13345: hupA - DNA-binding protein HU-alpha, at 2,565,664 to 2,565,936
hupA
OKFHMN_13350: yjaG - Uncharacterized protein YjaG, at 2,566,123 to 2,566,713
yjaG
OKFHMN_13355: nfi - deoxyribonuclease V, at 2,566,756 to 2,567,427
nfi
OKFHMN_13360: hemE - uroporphyrinogen decarboxylase, at 2,567,437 to 2,568,501
hemE
Position (kb)
2566
2567
2568 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2565.812 kb on - strand, within hupA at 2565.910 kb on + strand at 2565.911 kb on - strand at 2565.936 kb on + strand at 2565.936 kb on + strand at 2565.937 kb on - strand at 2565.941 kb on + strand at 2565.947 kb on + strand at 2565.998 kb on + strand at 2566.013 kb on + strand at 2566.053 kb on - strand at 2566.104 kb on + strand at 2566.105 kb on - strand at 2566.105 kb on - strand at 2566.124 kb on + strand at 2566.180 kb on - strand at 2566.180 kb on - strand at 2566.180 kb on - strand at 2566.190 kb on - strand, within yjaG at 2566.190 kb on - strand, within yjaG at 2566.264 kb on + strand, within yjaG at 2566.264 kb on + strand, within yjaG at 2566.265 kb on - strand, within yjaG at 2566.354 kb on - strand, within yjaG at 2566.354 kb on - strand, within yjaG at 2566.443 kb on - strand, within yjaG at 2566.517 kb on - strand, within yjaG at 2566.563 kb on + strand, within yjaG at 2566.564 kb on - strand, within yjaG at 2566.617 kb on - strand, within yjaG at 2566.708 kb on + strand at 2566.709 kb on - strand at 2566.773 kb on + strand at 2566.773 kb on + strand at 2566.775 kb on + strand at 2566.861 kb on + strand, within nfi at 2566.909 kb on - strand, within nfi at 2566.909 kb on - strand, within nfi at 2566.912 kb on + strand, within nfi at 2567.023 kb on + strand, within nfi at 2567.132 kb on - strand, within nfi at 2567.132 kb on - strand, within nfi at 2567.210 kb on + strand, within nfi at 2567.210 kb on + strand, within nfi at 2567.211 kb on - strand, within nfi at 2567.242 kb on - strand, within nfi at 2567.244 kb on - strand, within nfi at 2567.447 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,565,812 - hupA OKFHMN_13345 0.54 -0.1 2,565,910 + +1.0 2,565,911 - -1.0 2,565,936 + -0.9 2,565,936 + -0.4 2,565,937 - +0.0 2,565,941 + -2.4 2,565,947 + +0.2 2,565,998 + -1.0 2,566,013 + -0.2 2,566,053 - +0.9 2,566,104 + +2.9 2,566,105 - +0.2 2,566,105 - -0.2 2,566,124 + -0.5 2,566,180 - -1.2 2,566,180 - -0.4 2,566,180 - +0.1 2,566,190 - yjaG OKFHMN_13350 0.11 -1.6 2,566,190 - yjaG OKFHMN_13350 0.11 -1.1 2,566,264 + yjaG OKFHMN_13350 0.24 -1.3 2,566,264 + yjaG OKFHMN_13350 0.24 -1.3 2,566,265 - yjaG OKFHMN_13350 0.24 +0.0 2,566,354 - yjaG OKFHMN_13350 0.39 -2.8 2,566,354 - yjaG OKFHMN_13350 0.39 -0.2 2,566,443 - yjaG OKFHMN_13350 0.54 -2.7 2,566,517 - yjaG OKFHMN_13350 0.67 -0.2 2,566,563 + yjaG OKFHMN_13350 0.74 +0.4 2,566,564 - yjaG OKFHMN_13350 0.75 +0.6 2,566,617 - yjaG OKFHMN_13350 0.84 -1.3 2,566,708 + -0.3 2,566,709 - +0.2 2,566,773 + +0.0 2,566,773 + -0.2 2,566,775 + +0.4 2,566,861 + nfi OKFHMN_13355 0.16 -0.3 2,566,909 - nfi OKFHMN_13355 0.23 +1.3 2,566,909 - nfi OKFHMN_13355 0.23 +3.9 2,566,912 + nfi OKFHMN_13355 0.23 +1.1 2,567,023 + nfi OKFHMN_13355 0.40 -0.5 2,567,132 - nfi OKFHMN_13355 0.56 -0.3 2,567,132 - nfi OKFHMN_13355 0.56 +0.6 2,567,210 + nfi OKFHMN_13355 0.68 -0.0 2,567,210 + nfi OKFHMN_13355 0.68 +0.1 2,567,211 - nfi OKFHMN_13355 0.68 -0.2 2,567,242 - nfi OKFHMN_13355 0.72 +0.3 2,567,244 - nfi OKFHMN_13355 0.73 -0.1 2,567,447 + -0.6
Or see this region's nucleotide sequence