Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ampE and ampD overlap by 4 nucleotides ampD and nadC are separated by 87 nucleotides nadC and ppdD are separated by 202 nucleotides ppdD and gspE are separated by 9 nucleotides
OKFHMN_10520: ampE - beta-lactamase regulator AmpE, at 1,978,568 to 1,979,422
ampE
OKFHMN_10525: ampD - 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD, at 1,979,419 to 1,979,970
ampD
OKFHMN_10530: nadC - carboxylating nicotinate-nucleotide diphosphorylase, at 1,980,058 to 1,980,951
nadC
OKFHMN_10535: ppdD - prepilin peptidase-dependent pilin, at 1,981,154 to 1,981,594
ppdD
OKFHMN_10540: gspE - type II secretion system protein GspE, at 1,981,604 to 1,982,989
gspE
Position (kb)
1980
1981 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1979.117 kb on - strand, within ampE at 1979.117 kb on - strand, within ampE at 1979.122 kb on + strand, within ampE at 1979.142 kb on + strand, within ampE at 1979.143 kb on - strand, within ampE at 1979.143 kb on - strand, within ampE at 1979.180 kb on - strand, within ampE at 1979.232 kb on - strand, within ampE at 1979.255 kb on + strand, within ampE at 1979.402 kb on + strand at 1979.402 kb on + strand at 1979.410 kb on + strand at 1979.503 kb on - strand, within ampD at 1979.523 kb on + strand, within ampD at 1979.524 kb on - strand, within ampD at 1979.582 kb on + strand, within ampD at 1979.597 kb on + strand, within ampD at 1979.597 kb on + strand, within ampD at 1979.598 kb on - strand, within ampD at 1979.712 kb on - strand, within ampD at 1979.719 kb on + strand, within ampD at 1979.719 kb on + strand, within ampD at 1979.805 kb on + strand, within ampD at 1979.806 kb on - strand, within ampD at 1979.818 kb on + strand, within ampD at 1979.863 kb on - strand, within ampD at 1979.962 kb on + strand at 1979.963 kb on - strand at 1979.984 kb on - strand at 1979.984 kb on - strand at 1979.984 kb on - strand at 1979.984 kb on - strand at 1979.985 kb on + strand at 1979.985 kb on + strand at 1979.985 kb on + strand at 1979.986 kb on - strand at 1979.986 kb on - strand at 1979.986 kb on - strand at 1979.986 kb on - strand at 1979.986 kb on - strand at 1979.986 kb on - strand at 1980.012 kb on + strand at 1980.023 kb on + strand at 1980.023 kb on + strand at 1980.028 kb on + strand at 1980.054 kb on + strand at 1980.055 kb on - strand at 1980.291 kb on + strand, within nadC at 1980.320 kb on + strand, within nadC at 1980.321 kb on - strand, within nadC at 1980.456 kb on + strand, within nadC at 1980.456 kb on + strand, within nadC at 1980.470 kb on - strand, within nadC at 1980.628 kb on - strand, within nadC at 1980.628 kb on - strand, within nadC at 1980.704 kb on + strand, within nadC at 1980.765 kb on + strand, within nadC at 1980.765 kb on + strand, within nadC at 1980.888 kb on + strand at 1980.909 kb on + strand at 1980.909 kb on + strand at 1980.909 kb on + strand at 1980.909 kb on + strand at 1980.909 kb on + strand at 1980.909 kb on + strand at 1980.909 kb on + strand at 1980.909 kb on + strand at 1980.909 kb on + strand at 1980.910 kb on - strand at 1980.910 kb on - strand at 1980.910 kb on - strand at 1980.918 kb on - strand at 1980.966 kb on - strand at 1981.124 kb on + strand at 1981.199 kb on - strand, within ppdD at 1981.219 kb on - strand, within ppdD at 1981.232 kb on - strand, within ppdD at 1981.281 kb on + strand, within ppdD at 1981.281 kb on + strand, within ppdD at 1981.281 kb on + strand, within ppdD at 1981.282 kb on - strand, within ppdD at 1981.282 kb on - strand, within ppdD at 1981.341 kb on + strand, within ppdD at 1981.341 kb on + strand, within ppdD at 1981.341 kb on + strand, within ppdD at 1981.341 kb on + strand, within ppdD at 1981.342 kb on - strand, within ppdD at 1981.342 kb on - strand, within ppdD at 1981.342 kb on - strand, within ppdD at 1981.346 kb on - strand, within ppdD at 1981.394 kb on + strand, within ppdD at 1981.592 kb on + strand at 1981.593 kb on - strand at 1981.609 kb on + strand at 1981.610 kb on - strand at 1981.610 kb on - strand at 1981.818 kb on + strand, within gspE at 1981.819 kb on - strand, within gspE
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,979,117 - ampE OKFHMN_10520 0.64 +0.6 1,979,117 - ampE OKFHMN_10520 0.64 -0.4 1,979,122 + ampE OKFHMN_10520 0.65 +0.0 1,979,142 + ampE OKFHMN_10520 0.67 -0.0 1,979,143 - ampE OKFHMN_10520 0.67 -0.3 1,979,143 - ampE OKFHMN_10520 0.67 +0.4 1,979,180 - ampE OKFHMN_10520 0.72 +0.9 1,979,232 - ampE OKFHMN_10520 0.78 -1.5 1,979,255 + ampE OKFHMN_10520 0.80 +0.9 1,979,402 + -0.9 1,979,402 + -0.4 1,979,410 + -0.1 1,979,503 - ampD OKFHMN_10525 0.15 -0.7 1,979,523 + ampD OKFHMN_10525 0.19 -0.0 1,979,524 - ampD OKFHMN_10525 0.19 -0.5 1,979,582 + ampD OKFHMN_10525 0.30 +1.5 1,979,597 + ampD OKFHMN_10525 0.32 +0.7 1,979,597 + ampD OKFHMN_10525 0.32 -1.8 1,979,598 - ampD OKFHMN_10525 0.32 +0.6 1,979,712 - ampD OKFHMN_10525 0.53 -0.1 1,979,719 + ampD OKFHMN_10525 0.54 -1.2 1,979,719 + ampD OKFHMN_10525 0.54 -0.4 1,979,805 + ampD OKFHMN_10525 0.70 +0.3 1,979,806 - ampD OKFHMN_10525 0.70 +0.4 1,979,818 + ampD OKFHMN_10525 0.72 +0.8 1,979,863 - ampD OKFHMN_10525 0.80 -1.3 1,979,962 + +0.1 1,979,963 - +0.0 1,979,984 - +1.2 1,979,984 - +0.4 1,979,984 - -0.0 1,979,984 - -0.1 1,979,985 + -0.2 1,979,985 + +1.0 1,979,985 + -0.1 1,979,986 - +0.2 1,979,986 - +0.0 1,979,986 - +0.1 1,979,986 - -0.0 1,979,986 - -1.7 1,979,986 - +0.3 1,980,012 + +0.0 1,980,023 + +0.2 1,980,023 + -0.0 1,980,028 + -1.2 1,980,054 + -2.0 1,980,055 - -1.8 1,980,291 + nadC OKFHMN_10530 0.26 -1.9 1,980,320 + nadC OKFHMN_10530 0.29 -1.7 1,980,321 - nadC OKFHMN_10530 0.29 -3.4 1,980,456 + nadC OKFHMN_10530 0.45 -3.2 1,980,456 + nadC OKFHMN_10530 0.45 -2.1 1,980,470 - nadC OKFHMN_10530 0.46 -1.8 1,980,628 - nadC OKFHMN_10530 0.64 -2.1 1,980,628 - nadC OKFHMN_10530 0.64 -2.6 1,980,704 + nadC OKFHMN_10530 0.72 -2.4 1,980,765 + nadC OKFHMN_10530 0.79 -2.6 1,980,765 + nadC OKFHMN_10530 0.79 -2.2 1,980,888 + -0.4 1,980,909 + -0.3 1,980,909 + -4.3 1,980,909 + -4.2 1,980,909 + -2.6 1,980,909 + -1.5 1,980,909 + -3.4 1,980,909 + -1.3 1,980,909 + -2.1 1,980,909 + -0.6 1,980,910 - -4.7 1,980,910 - -0.9 1,980,910 - -1.3 1,980,918 - -2.4 1,980,966 - +0.0 1,981,124 + +0.2 1,981,199 - ppdD OKFHMN_10535 0.10 -1.2 1,981,219 - ppdD OKFHMN_10535 0.15 -0.0 1,981,232 - ppdD OKFHMN_10535 0.18 -0.4 1,981,281 + ppdD OKFHMN_10535 0.29 -0.8 1,981,281 + ppdD OKFHMN_10535 0.29 -1.1 1,981,281 + ppdD OKFHMN_10535 0.29 -0.0 1,981,282 - ppdD OKFHMN_10535 0.29 +0.1 1,981,282 - ppdD OKFHMN_10535 0.29 +0.6 1,981,341 + ppdD OKFHMN_10535 0.42 +0.7 1,981,341 + ppdD OKFHMN_10535 0.42 -1.8 1,981,341 + ppdD OKFHMN_10535 0.42 -0.3 1,981,341 + ppdD OKFHMN_10535 0.42 +0.8 1,981,342 - ppdD OKFHMN_10535 0.43 +2.4 1,981,342 - ppdD OKFHMN_10535 0.43 -0.2 1,981,342 - ppdD OKFHMN_10535 0.43 -1.0 1,981,346 - ppdD OKFHMN_10535 0.44 -1.7 1,981,394 + ppdD OKFHMN_10535 0.54 -0.3 1,981,592 + +0.5 1,981,593 - -1.1 1,981,609 + -0.4 1,981,610 - -1.1 1,981,610 - +2.5 1,981,818 + gspE OKFHMN_10540 0.15 +0.3 1,981,819 - gspE OKFHMN_10540 0.16 -1.3
Or see this region's nucleotide sequence