Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt speD and yacL are separated by 37 nucleotides yacL and acnB are separated by 175 nucleotides acnB and yacH are separated by 354 nucleotides
OKFHMN_10470: speD - adenosylmethionine decarboxylase, at 1,963,238 to 1,964,032
speD
OKFHMN_10475: yacL - protein YacL, at 1,964,070 to 1,964,432
yacL
OKFHMN_10480: acnB - bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase, at 1,964,608 to 1,967,205
acnB
OKFHMN_10485: yacH - Uncharacterized protein YacH, at 1,967,560 to 1,969,413
yacH
Position (kb)
1964
1965
1966
1967
1968 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1963.624 kb on + strand, within speD at 1963.625 kb on - strand, within speD at 1963.688 kb on + strand, within speD at 1963.689 kb on - strand, within speD at 1963.719 kb on + strand, within speD at 1963.811 kb on - strand, within speD at 1963.822 kb on + strand, within speD at 1963.823 kb on - strand, within speD at 1963.851 kb on + strand, within speD at 1963.851 kb on + strand, within speD at 1963.916 kb on + strand, within speD at 1963.916 kb on + strand, within speD at 1964.244 kb on - strand, within yacL at 1964.244 kb on - strand, within yacL at 1964.244 kb on - strand, within yacL at 1964.244 kb on - strand, within yacL at 1964.245 kb on + strand, within yacL at 1964.246 kb on - strand, within yacL at 1964.246 kb on - strand, within yacL at 1964.246 kb on - strand, within yacL at 1964.348 kb on + strand, within yacL at 1964.384 kb on + strand, within yacL at 1964.385 kb on - strand, within yacL at 1964.394 kb on + strand, within yacL at 1964.432 kb on + strand at 1964.486 kb on + strand at 1964.487 kb on - strand at 1964.487 kb on - strand at 1964.487 kb on - strand at 1964.487 kb on - strand at 1964.491 kb on - strand at 1964.685 kb on - strand at 1965.385 kb on - strand, within acnB at 1965.385 kb on - strand, within acnB at 1967.233 kb on - strand at 1967.285 kb on - strand at 1967.285 kb on - strand at 1967.389 kb on + strand at 1967.390 kb on - strand at 1967.491 kb on + strand at 1967.639 kb on + strand at 1967.641 kb on + strand at 1967.641 kb on + strand at 1967.642 kb on - strand at 1967.642 kb on - strand at 1967.642 kb on - strand at 1967.644 kb on - strand at 1967.645 kb on + strand at 1967.645 kb on + strand at 1967.646 kb on - strand at 1967.646 kb on - strand at 1967.646 kb on - strand at 1967.718 kb on + strand at 1967.793 kb on - strand, within yacH at 1967.904 kb on + strand, within yacH at 1967.913 kb on + strand, within yacH at 1967.942 kb on + strand, within yacH at 1967.971 kb on - strand, within yacH at 1967.971 kb on - strand, within yacH at 1968.083 kb on + strand, within yacH
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,963,624 + speD OKFHMN_10470 0.49 +0.2 1,963,625 - speD OKFHMN_10470 0.49 -3.6 1,963,688 + speD OKFHMN_10470 0.57 -0.6 1,963,689 - speD OKFHMN_10470 0.57 -0.4 1,963,719 + speD OKFHMN_10470 0.61 +0.3 1,963,811 - speD OKFHMN_10470 0.72 -0.8 1,963,822 + speD OKFHMN_10470 0.73 -0.8 1,963,823 - speD OKFHMN_10470 0.74 +1.1 1,963,851 + speD OKFHMN_10470 0.77 -0.8 1,963,851 + speD OKFHMN_10470 0.77 -0.3 1,963,916 + speD OKFHMN_10470 0.85 +0.3 1,963,916 + speD OKFHMN_10470 0.85 -2.7 1,964,244 - yacL OKFHMN_10475 0.48 +0.1 1,964,244 - yacL OKFHMN_10475 0.48 +0.0 1,964,244 - yacL OKFHMN_10475 0.48 -1.8 1,964,244 - yacL OKFHMN_10475 0.48 -0.1 1,964,245 + yacL OKFHMN_10475 0.48 -0.5 1,964,246 - yacL OKFHMN_10475 0.48 -0.9 1,964,246 - yacL OKFHMN_10475 0.48 -1.0 1,964,246 - yacL OKFHMN_10475 0.48 +0.3 1,964,348 + yacL OKFHMN_10475 0.77 +1.0 1,964,384 + yacL OKFHMN_10475 0.87 +0.2 1,964,385 - yacL OKFHMN_10475 0.87 +1.4 1,964,394 + yacL OKFHMN_10475 0.89 -0.4 1,964,432 + -1.0 1,964,486 + +0.1 1,964,487 - -0.5 1,964,487 - +0.6 1,964,487 - +0.4 1,964,487 - -0.7 1,964,491 - -0.4 1,964,685 - -0.6 1,965,385 - acnB OKFHMN_10480 0.30 -0.8 1,965,385 - acnB OKFHMN_10480 0.30 -1.1 1,967,233 - -2.6 1,967,285 - +3.2 1,967,285 - +0.4 1,967,389 + +0.2 1,967,390 - +0.6 1,967,491 + -0.4 1,967,639 + +1.2 1,967,641 + -3.1 1,967,641 + -1.0 1,967,642 - +0.4 1,967,642 - -0.6 1,967,642 - -0.4 1,967,644 - -0.3 1,967,645 + +0.6 1,967,645 + -0.6 1,967,646 - -0.4 1,967,646 - +0.5 1,967,646 - +0.5 1,967,718 + -0.2 1,967,793 - yacH OKFHMN_10485 0.13 -0.6 1,967,904 + yacH OKFHMN_10485 0.19 -0.6 1,967,913 + yacH OKFHMN_10485 0.19 -0.8 1,967,942 + yacH OKFHMN_10485 0.21 -0.6 1,967,971 - yacH OKFHMN_10485 0.22 +0.3 1,967,971 - yacH OKFHMN_10485 0.22 +1.2 1,968,083 + yacH OKFHMN_10485 0.28 -0.5
Or see this region's nucleotide sequence