Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Asp_trna and dnaQ are separated by 132 nucleotides dnaQ and rnhA are separated by 55 nucleotides rnhA and yafS overlap by 4 nucleotides yafS and gloB are separated by 33 nucleotides
OKFHMN_09960: Asp_trna - tRNA-Asp(gtc), at 1,862,635 to 1,862,711
_trna
OKFHMN_09965: dnaQ - DNA polymerase III subunit epsilon, at 1,862,844 to 1,863,584
dnaQ
OKFHMN_09970: rnhA - ribonuclease HI, at 1,863,640 to 1,864,107
rnhA
OKFHMN_09975: yafS - Uncharacterized protein YafS, at 1,864,104 to 1,864,826
yafS
OKFHMN_09980: gloB - hydroxyacylglutathione hydrolase, at 1,864,860 to 1,865,615
gloB
Position (kb)
1863
1864
1865 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1862.841 kb on + strand at 1862.846 kb on - strand at 1863.645 kb on + strand at 1863.645 kb on + strand at 1863.815 kb on + strand, within rnhA at 1863.870 kb on + strand, within rnhA at 1864.083 kb on + strand at 1864.118 kb on + strand at 1864.223 kb on + strand, within yafS at 1864.230 kb on + strand, within yafS at 1864.231 kb on - strand, within yafS at 1864.231 kb on - strand, within yafS at 1864.251 kb on - strand, within yafS at 1864.314 kb on + strand, within yafS at 1864.315 kb on - strand, within yafS at 1864.532 kb on + strand, within yafS at 1864.532 kb on + strand, within yafS at 1864.532 kb on + strand, within yafS at 1864.532 kb on + strand, within yafS at 1864.533 kb on - strand, within yafS at 1864.533 kb on - strand, within yafS at 1864.706 kb on - strand, within yafS at 1864.854 kb on + strand at 1864.854 kb on + strand at 1864.855 kb on - strand at 1864.860 kb on - strand at 1864.860 kb on - strand at 1864.875 kb on - strand at 1864.875 kb on - strand at 1864.897 kb on - strand at 1864.897 kb on - strand at 1864.897 kb on - strand at 1864.897 kb on - strand at 1864.897 kb on - strand at 1864.964 kb on - strand, within gloB at 1865.006 kb on - strand, within gloB at 1865.006 kb on - strand, within gloB at 1865.006 kb on - strand, within gloB at 1865.006 kb on - strand, within gloB at 1865.082 kb on + strand, within gloB at 1865.083 kb on - strand, within gloB at 1865.083 kb on - strand, within gloB at 1865.083 kb on - strand, within gloB at 1865.083 kb on - strand, within gloB at 1865.106 kb on - strand, within gloB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,862,841 + -0.9 1,862,846 - +0.3 1,863,645 + -0.5 1,863,645 + -0.9 1,863,815 + rnhA OKFHMN_09970 0.37 +0.1 1,863,870 + rnhA OKFHMN_09970 0.49 -0.0 1,864,083 + +0.6 1,864,118 + +0.3 1,864,223 + yafS OKFHMN_09975 0.16 -0.0 1,864,230 + yafS OKFHMN_09975 0.17 -0.3 1,864,231 - yafS OKFHMN_09975 0.18 +0.0 1,864,231 - yafS OKFHMN_09975 0.18 +1.7 1,864,251 - yafS OKFHMN_09975 0.20 -1.7 1,864,314 + yafS OKFHMN_09975 0.29 +0.3 1,864,315 - yafS OKFHMN_09975 0.29 -0.5 1,864,532 + yafS OKFHMN_09975 0.59 -0.4 1,864,532 + yafS OKFHMN_09975 0.59 -2.5 1,864,532 + yafS OKFHMN_09975 0.59 -0.1 1,864,532 + yafS OKFHMN_09975 0.59 +0.6 1,864,533 - yafS OKFHMN_09975 0.59 +0.1 1,864,533 - yafS OKFHMN_09975 0.59 +0.1 1,864,706 - yafS OKFHMN_09975 0.83 -1.7 1,864,854 + -0.2 1,864,854 + -0.9 1,864,855 - -0.3 1,864,860 - +0.2 1,864,860 - +0.2 1,864,875 - -3.1 1,864,875 - -1.5 1,864,897 - -0.5 1,864,897 - +1.3 1,864,897 - +0.2 1,864,897 - +0.2 1,864,897 - +0.5 1,864,964 - gloB OKFHMN_09980 0.14 +0.5 1,865,006 - gloB OKFHMN_09980 0.19 -0.3 1,865,006 - gloB OKFHMN_09980 0.19 -0.3 1,865,006 - gloB OKFHMN_09980 0.19 -1.9 1,865,006 - gloB OKFHMN_09980 0.19 +0.4 1,865,082 + gloB OKFHMN_09980 0.29 -0.3 1,865,083 - gloB OKFHMN_09980 0.29 -0.9 1,865,083 - gloB OKFHMN_09980 0.29 +1.3 1,865,083 - gloB OKFHMN_09980 0.29 -0.3 1,865,083 - gloB OKFHMN_09980 0.29 +1.4 1,865,106 - gloB OKFHMN_09980 0.33 +0.6
Or see this region's nucleotide sequence