Strain Fitness in Escherichia coli ECRC100 around OKFHMN_09715

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntprfH and ykfJ overlap by 32 nucleotidesykfJ and yafP are separated by 306 nucleotidesyafP and OKFHMN_09720 are separated by 170 nucleotidesOKFHMN_09720 and yafN are separated by 2 nucleotidesyafN and dinB are separated by 51 nucleotides OKFHMN_09705: prfH - peptide chain release factor H, at 1,808,547 to 1,809,047 prfH OKFHMN_09710: ykfJ - Putative uncharacterized protein YkfJ, at 1,809,016 to 1,809,282 ykfJ OKFHMN_09715: yafP - GNAT family N-acetyltransferase, at 1,809,589 to 1,809,966 yafP OKFHMN_09720: OKFHMN_09720 - type II toxin-antitoxin system YafO family toxin, at 1,810,137 to 1,810,448 _09720 OKFHMN_09725: yafN - type I toxin-antitoxin system antitoxin YafN, at 1,810,451 to 1,810,744 yafN OKFHMN_09730: dinB - DNA polymerase IV, at 1,810,796 to 1,811,851 dinB Position (kb) 1809 1810Strain fitness (log2 ratio) -2 -1 0 1 2at 1809.127 kb on + strand, within ykfJat 1809.182 kb on - strand, within ykfJat 1809.182 kb on - strand, within ykfJat 1809.232 kb on - strand, within ykfJat 1809.260 kb on + strandat 1809.261 kb on - strandat 1809.272 kb on + strandat 1809.273 kb on - strandat 1809.273 kb on - strandat 1809.284 kb on + strandat 1809.296 kb on - strandat 1809.312 kb on - strandat 1809.352 kb on - strandat 1809.409 kb on + strandat 1809.569 kb on - strandat 1809.606 kb on - strandat 1809.804 kb on + strand, within yafPat 1809.811 kb on - strand, within yafPat 1809.813 kb on - strand, within yafPat 1810.024 kb on + strandat 1810.030 kb on + strandat 1810.030 kb on + strandat 1810.031 kb on - strandat 1810.046 kb on + strandat 1810.108 kb on - strandat 1810.190 kb on - strand, within OKFHMN_09720at 1810.338 kb on + strand, within OKFHMN_09720at 1810.339 kb on - strand, within OKFHMN_09720at 1810.365 kb on - strand, within OKFHMN_09720at 1810.366 kb on + strand, within OKFHMN_09720at 1810.487 kb on - strand, within yafNat 1810.646 kb on - strand, within yafNat 1810.676 kb on - strand, within yafNat 1810.680 kb on + strand, within yafNat 1810.701 kb on - strand, within yafNat 1810.745 kb on - strandat 1810.792 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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1,809,127 + ykfJ OKFHMN_09710 0.42 -0.3
1,809,182 - ykfJ OKFHMN_09710 0.62 +0.9
1,809,182 - ykfJ OKFHMN_09710 0.62 +0.1
1,809,232 - ykfJ OKFHMN_09710 0.81 +0.3
1,809,260 + +1.3
1,809,261 - -1.3
1,809,272 + +0.5
1,809,273 - +0.2
1,809,273 - +0.3
1,809,284 + -0.0
1,809,296 - -1.2
1,809,312 - +0.6
1,809,352 - -0.5
1,809,409 + +0.2
1,809,569 - +1.8
1,809,606 - +0.4
1,809,804 + yafP OKFHMN_09715 0.57 +0.0
1,809,811 - yafP OKFHMN_09715 0.59 +0.3
1,809,813 - yafP OKFHMN_09715 0.59 -0.3
1,810,024 + -0.8
1,810,030 + -0.3
1,810,030 + +0.4
1,810,031 - +0.5
1,810,046 + -0.3
1,810,108 - +0.8
1,810,190 - OKFHMN_09720 0.17 +0.1
1,810,338 + OKFHMN_09720 0.64 +0.3
1,810,339 - OKFHMN_09720 0.65 -1.8
1,810,365 - OKFHMN_09720 0.73 -0.8
1,810,366 + OKFHMN_09720 0.73 +1.1
1,810,487 - yafN OKFHMN_09725 0.12 +0.3
1,810,646 - yafN OKFHMN_09725 0.66 +2.0
1,810,676 - yafN OKFHMN_09725 0.77 -0.1
1,810,680 + yafN OKFHMN_09725 0.78 -0.2
1,810,701 - yafN OKFHMN_09725 0.85 -1.0
1,810,745 - +0.3
1,810,792 + +0.1

Or see this region's nucleotide sequence