Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt codA and codB overlap by 11 nucleotides codB and prpE are separated by 893 nucleotides
OKFHMN_08845: codA - cytosine/isoguanine deaminase, at 1,658,571 to 1,659,854
codA
OKFHMN_08850: codB - cytosine permease, at 1,659,844 to 1,661,103
codB
OKFHMN_08885: prpE - propionate--CoA ligase, at 1,661,997 to 1,663,883
prpE
Position (kb)
1659
1660
1661
1662 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1658.934 kb on - strand, within codA at 1658.934 kb on - strand, within codA at 1658.987 kb on - strand, within codA at 1659.044 kb on + strand, within codA at 1659.083 kb on - strand, within codA at 1659.092 kb on + strand, within codA at 1659.094 kb on + strand, within codA at 1659.095 kb on - strand, within codA at 1659.258 kb on - strand, within codA at 1659.258 kb on - strand, within codA at 1659.258 kb on - strand, within codA at 1659.315 kb on + strand, within codA at 1659.333 kb on + strand, within codA at 1659.334 kb on - strand, within codA at 1659.570 kb on + strand, within codA at 1659.669 kb on - strand, within codA at 1659.669 kb on - strand, within codA at 1659.691 kb on - strand, within codA at 1659.696 kb on + strand, within codA at 1659.827 kb on + strand at 1659.827 kb on + strand at 1659.845 kb on + strand at 1659.858 kb on + strand at 1659.859 kb on - strand at 1659.921 kb on - strand at 1659.921 kb on - strand at 1659.937 kb on + strand at 1659.937 kb on + strand at 1659.937 kb on + strand at 1660.055 kb on + strand, within codB at 1660.056 kb on - strand, within codB at 1660.076 kb on + strand, within codB at 1660.110 kb on + strand, within codB at 1660.110 kb on + strand, within codB at 1660.111 kb on - strand, within codB at 1660.152 kb on - strand, within codB at 1660.160 kb on - strand, within codB at 1660.183 kb on - strand, within codB at 1660.283 kb on - strand, within codB at 1660.342 kb on - strand, within codB at 1660.492 kb on - strand, within codB at 1660.531 kb on + strand, within codB at 1660.558 kb on + strand, within codB at 1660.719 kb on + strand, within codB at 1660.720 kb on - strand, within codB at 1660.889 kb on + strand, within codB at 1660.907 kb on + strand, within codB at 1660.908 kb on - strand, within codB at 1660.912 kb on - strand, within codB at 1660.963 kb on - strand, within codB at 1660.995 kb on + strand at 1660.995 kb on + strand at 1661.152 kb on + strand at 1661.153 kb on - strand at 1661.204 kb on + strand at 1661.257 kb on + strand at 1661.296 kb on - strand at 1661.360 kb on + strand at 1661.454 kb on + strand at 1661.454 kb on + strand at 1661.454 kb on + strand at 1661.632 kb on + strand at 1661.998 kb on + strand at 1661.999 kb on - strand at 1661.999 kb on - strand at 1661.999 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,658,934 - codA OKFHMN_08845 0.28 +0.2 1,658,934 - codA OKFHMN_08845 0.28 +0.0 1,658,987 - codA OKFHMN_08845 0.32 +1.5 1,659,044 + codA OKFHMN_08845 0.37 -1.9 1,659,083 - codA OKFHMN_08845 0.40 -1.5 1,659,092 + codA OKFHMN_08845 0.41 +0.5 1,659,094 + codA OKFHMN_08845 0.41 -0.6 1,659,095 - codA OKFHMN_08845 0.41 +0.0 1,659,258 - codA OKFHMN_08845 0.54 +0.3 1,659,258 - codA OKFHMN_08845 0.54 -0.2 1,659,258 - codA OKFHMN_08845 0.54 +0.4 1,659,315 + codA OKFHMN_08845 0.58 -0.8 1,659,333 + codA OKFHMN_08845 0.59 +0.9 1,659,334 - codA OKFHMN_08845 0.59 -2.3 1,659,570 + codA OKFHMN_08845 0.78 +0.1 1,659,669 - codA OKFHMN_08845 0.86 +0.6 1,659,669 - codA OKFHMN_08845 0.86 +0.3 1,659,691 - codA OKFHMN_08845 0.87 +0.0 1,659,696 + codA OKFHMN_08845 0.88 +0.1 1,659,827 + +0.5 1,659,827 + +0.1 1,659,845 + +0.7 1,659,858 + -0.7 1,659,859 - -0.2 1,659,921 - +0.2 1,659,921 - +0.5 1,659,937 + -0.7 1,659,937 + +1.0 1,659,937 + +0.7 1,660,055 + codB OKFHMN_08850 0.17 -0.5 1,660,056 - codB OKFHMN_08850 0.17 +0.1 1,660,076 + codB OKFHMN_08850 0.18 -0.5 1,660,110 + codB OKFHMN_08850 0.21 -0.0 1,660,110 + codB OKFHMN_08850 0.21 +0.2 1,660,111 - codB OKFHMN_08850 0.21 -0.2 1,660,152 - codB OKFHMN_08850 0.24 -0.7 1,660,160 - codB OKFHMN_08850 0.25 +0.2 1,660,183 - codB OKFHMN_08850 0.27 +1.2 1,660,283 - codB OKFHMN_08850 0.35 +0.7 1,660,342 - codB OKFHMN_08850 0.40 +0.7 1,660,492 - codB OKFHMN_08850 0.51 +0.9 1,660,531 + codB OKFHMN_08850 0.55 -0.4 1,660,558 + codB OKFHMN_08850 0.57 -2.9 1,660,719 + codB OKFHMN_08850 0.69 +1.3 1,660,720 - codB OKFHMN_08850 0.70 -0.0 1,660,889 + codB OKFHMN_08850 0.83 -0.8 1,660,907 + codB OKFHMN_08850 0.84 +0.0 1,660,908 - codB OKFHMN_08850 0.84 +0.8 1,660,912 - codB OKFHMN_08850 0.85 -1.3 1,660,963 - codB OKFHMN_08850 0.89 +0.6 1,660,995 + +0.6 1,660,995 + +0.2 1,661,152 + -1.1 1,661,153 - +0.2 1,661,204 + +0.3 1,661,257 + -0.1 1,661,296 - +1.8 1,661,360 + +0.9 1,661,454 + +0.2 1,661,454 + -1.9 1,661,454 + +0.7 1,661,632 + +0.7 1,661,998 + -0.2 1,661,999 - +0.2 1,661,999 - -0.5 1,661,999 - -0.2
Or see this region's nucleotide sequence