Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nei and pxpA are separated by 35 nucleotides pxpA and pxpC overlap by 11 nucleotides pxpC and pxpB overlap by 7 nucleotides pxpB and ybgI are separated by 22 nucleotides
OKFHMN_06990: nei - endonuclease VIII, at 1,249,932 to 1,250,723
nei
OKFHMN_06995: pxpA - 5-oxoprolinase subunit PxpA, at 1,250,759 to 1,251,493
pxpA
OKFHMN_07000: pxpC - 5-oxoprolinase subunit PxpC, at 1,251,483 to 1,252,415
pxpC
OKFHMN_07005: pxpB - 5-oxoprolinase subunit PxpB, at 1,252,409 to 1,253,065
pxpB
OKFHMN_07010: ybgI - radiation resistance protein YbgI, at 1,253,088 to 1,253,831
ybgI
Position (kb)
1251
1252
1253 Strain fitness (log2 ratio)
-2
-1
0
1 at 1250.528 kb on - strand, within nei at 1250.536 kb on - strand, within nei at 1250.556 kb on + strand, within nei at 1250.556 kb on + strand, within nei at 1250.557 kb on - strand, within nei at 1250.589 kb on - strand, within nei at 1250.617 kb on + strand, within nei at 1250.747 kb on + strand at 1250.761 kb on - strand at 1250.802 kb on - strand at 1251.112 kb on - strand, within pxpA at 1251.114 kb on - strand, within pxpA at 1251.165 kb on + strand, within pxpA at 1251.166 kb on - strand, within pxpA at 1251.198 kb on - strand, within pxpA at 1251.243 kb on + strand, within pxpA at 1251.259 kb on - strand, within pxpA at 1251.438 kb on - strand at 1251.438 kb on - strand at 1251.510 kb on + strand at 1251.620 kb on + strand, within pxpC at 1251.620 kb on + strand, within pxpC at 1251.621 kb on - strand, within pxpC at 1251.636 kb on - strand, within pxpC at 1251.645 kb on - strand, within pxpC at 1251.816 kb on - strand, within pxpC at 1251.872 kb on - strand, within pxpC at 1252.167 kb on - strand, within pxpC at 1252.167 kb on - strand, within pxpC at 1252.167 kb on - strand, within pxpC at 1252.316 kb on + strand, within pxpC at 1252.386 kb on - strand at 1252.388 kb on - strand at 1252.388 kb on - strand at 1252.415 kb on + strand at 1252.460 kb on + strand at 1252.464 kb on - strand at 1252.508 kb on - strand, within pxpB at 1252.629 kb on - strand, within pxpB at 1252.909 kb on + strand, within pxpB at 1252.910 kb on - strand, within pxpB at 1253.047 kb on - strand at 1253.089 kb on + strand at 1253.090 kb on - strand at 1253.090 kb on - strand at 1253.166 kb on - strand, within ybgI at 1253.201 kb on - strand, within ybgI at 1253.375 kb on + strand, within ybgI
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,250,528 - nei OKFHMN_06990 0.75 +0.6 1,250,536 - nei OKFHMN_06990 0.76 -0.3 1,250,556 + nei OKFHMN_06990 0.79 +0.9 1,250,556 + nei OKFHMN_06990 0.79 -1.4 1,250,557 - nei OKFHMN_06990 0.79 +1.5 1,250,589 - nei OKFHMN_06990 0.83 -0.5 1,250,617 + nei OKFHMN_06990 0.86 +0.7 1,250,747 + -1.7 1,250,761 - -0.3 1,250,802 - -0.2 1,251,112 - pxpA OKFHMN_06995 0.48 -0.2 1,251,114 - pxpA OKFHMN_06995 0.48 -0.2 1,251,165 + pxpA OKFHMN_06995 0.55 +0.3 1,251,166 - pxpA OKFHMN_06995 0.55 +0.4 1,251,198 - pxpA OKFHMN_06995 0.60 +1.5 1,251,243 + pxpA OKFHMN_06995 0.66 +0.3 1,251,259 - pxpA OKFHMN_06995 0.68 +1.0 1,251,438 - +0.5 1,251,438 - -0.4 1,251,510 + -0.6 1,251,620 + pxpC OKFHMN_07000 0.15 -0.8 1,251,620 + pxpC OKFHMN_07000 0.15 +1.1 1,251,621 - pxpC OKFHMN_07000 0.15 -0.3 1,251,636 - pxpC OKFHMN_07000 0.16 +1.2 1,251,645 - pxpC OKFHMN_07000 0.17 -1.4 1,251,816 - pxpC OKFHMN_07000 0.36 -0.6 1,251,872 - pxpC OKFHMN_07000 0.42 +0.2 1,252,167 - pxpC OKFHMN_07000 0.73 -0.2 1,252,167 - pxpC OKFHMN_07000 0.73 -2.4 1,252,167 - pxpC OKFHMN_07000 0.73 -0.3 1,252,316 + pxpC OKFHMN_07000 0.89 -0.2 1,252,386 - -0.3 1,252,388 - +0.7 1,252,388 - +0.3 1,252,415 + -0.6 1,252,460 + -0.1 1,252,464 - +1.2 1,252,508 - pxpB OKFHMN_07005 0.15 -0.8 1,252,629 - pxpB OKFHMN_07005 0.33 +0.1 1,252,909 + pxpB OKFHMN_07005 0.76 -0.0 1,252,910 - pxpB OKFHMN_07005 0.76 -0.1 1,253,047 - +0.3 1,253,089 + +0.2 1,253,090 - -0.5 1,253,090 - -1.4 1,253,166 - ybgI OKFHMN_07010 0.10 -0.1 1,253,201 - ybgI OKFHMN_07010 0.15 +0.1 1,253,375 + ybgI OKFHMN_07010 0.39 +0.3
Or see this region's nucleotide sequence