Strain Fitness in Escherichia coli ECRC100 around OKFHMN_06135

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmoeB and fsa are separated by 178 nucleotidesfsa and ybiY are separated by 130 nucleotidesybiY and OKFHMN_06140 are separated by 5 nucleotidesOKFHMN_06140 and pflD are separated by 77 nucleotides OKFHMN_06120: moeB - molybdopterin-synthase adenylyltransferase MoeB, at 1,083,811 to 1,084,560 moeB OKFHMN_06130: fsa - fructose-6-phosphate aldolase, at 1,084,739 to 1,085,401 fsa OKFHMN_06135: ybiY - Putative pyruvate formate-lyase 3-activating enzyme, at 1,085,532 to 1,086,431 ybiY OKFHMN_06140: OKFHMN_06140 - Putative formate acetyltransferase 3 (Pyruvate formate lyase 3), at 1,086,437 to 1,086,991 _06140 OKFHMN_06145: pflD - Pyruvate-formate lyase, at 1,087,069 to 1,088,868 pflD Position (kb) 1085 1086 1087Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1084.580 kb on + strandat 1084.718 kb on - strandat 1084.816 kb on + strand, within fsaat 1084.955 kb on + strand, within fsaat 1085.000 kb on - strand, within fsaat 1085.023 kb on - strand, within fsaat 1085.023 kb on - strand, within fsaat 1085.114 kb on - strand, within fsaat 1085.168 kb on - strand, within fsaat 1085.171 kb on - strand, within fsaat 1085.321 kb on - strand, within fsaat 1085.435 kb on + strandat 1085.435 kb on + strandat 1085.435 kb on + strandat 1085.436 kb on - strandat 1085.436 kb on - strandat 1085.441 kb on - strandat 1085.531 kb on + strandat 1085.532 kb on - strandat 1085.754 kb on - strand, within ybiYat 1085.754 kb on - strand, within ybiYat 1086.052 kb on - strand, within ybiYat 1086.319 kb on + strand, within ybiYat 1086.320 kb on - strand, within ybiYat 1086.323 kb on + strand, within ybiYat 1086.503 kb on - strand, within OKFHMN_06140at 1086.543 kb on - strand, within OKFHMN_06140at 1086.545 kb on - strand, within OKFHMN_06140at 1086.555 kb on - strand, within OKFHMN_06140at 1086.585 kb on + strand, within OKFHMN_06140at 1086.705 kb on - strand, within OKFHMN_06140at 1086.706 kb on + strand, within OKFHMN_06140at 1087.309 kb on + strand, within pflDat 1087.309 kb on + strand, within pflDat 1087.322 kb on - strand, within pflDat 1087.386 kb on + strand, within pflD

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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1,084,580 + +0.8
1,084,718 - +0.4
1,084,816 + fsa OKFHMN_06130 0.12 +0.5
1,084,955 + fsa OKFHMN_06130 0.33 +0.2
1,085,000 - fsa OKFHMN_06130 0.39 +1.6
1,085,023 - fsa OKFHMN_06130 0.43 +0.6
1,085,023 - fsa OKFHMN_06130 0.43 +0.3
1,085,114 - fsa OKFHMN_06130 0.57 -1.1
1,085,168 - fsa OKFHMN_06130 0.65 -3.8
1,085,171 - fsa OKFHMN_06130 0.65 -0.3
1,085,321 - fsa OKFHMN_06130 0.88 -0.6
1,085,435 + +1.0
1,085,435 + -2.0
1,085,435 + -0.7
1,085,436 - +0.4
1,085,436 - -0.3
1,085,441 - +1.8
1,085,531 + -0.8
1,085,532 - +0.0
1,085,754 - ybiY OKFHMN_06135 0.25 -0.6
1,085,754 - ybiY OKFHMN_06135 0.25 +1.4
1,086,052 - ybiY OKFHMN_06135 0.58 -0.5
1,086,319 + ybiY OKFHMN_06135 0.87 +0.5
1,086,320 - ybiY OKFHMN_06135 0.88 +0.1
1,086,323 + ybiY OKFHMN_06135 0.88 +0.0
1,086,503 - OKFHMN_06140 0.12 -1.6
1,086,543 - OKFHMN_06140 0.19 -0.3
1,086,545 - OKFHMN_06140 0.19 -0.5
1,086,555 - OKFHMN_06140 0.21 +0.3
1,086,585 + OKFHMN_06140 0.27 -1.6
1,086,705 - OKFHMN_06140 0.48 -0.5
1,086,706 + OKFHMN_06140 0.48 +0.3
1,087,309 + pflD OKFHMN_06145 0.13 -1.8
1,087,309 + pflD OKFHMN_06145 0.13 +0.3
1,087,322 - pflD OKFHMN_06145 0.14 +0.1
1,087,386 + pflD OKFHMN_06145 0.18 -3.6

Or see this region's nucleotide sequence