Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt moeA and moeB overlap by 1 nucleotides moeB and fsa are separated by 178 nucleotides fsa and ybiY are separated by 130 nucleotides
OKFHMN_06115: moeA - molybdopterin molybdotransferase MoeA, at 1,082,576 to 1,083,811
moeA
OKFHMN_06120: moeB - molybdopterin-synthase adenylyltransferase MoeB, at 1,083,811 to 1,084,560
moeB
OKFHMN_06130: fsa - fructose-6-phosphate aldolase, at 1,084,739 to 1,085,401
fsa
OKFHMN_06135: ybiY - Putative pyruvate formate-lyase 3-activating enzyme, at 1,085,532 to 1,086,431
ybiY
Position (kb)
1084
1085
1086 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1083.777 kb on + strand at 1083.845 kb on - strand at 1084.050 kb on + strand, within moeB at 1084.051 kb on - strand, within moeB at 1084.051 kb on - strand, within moeB at 1084.194 kb on + strand, within moeB at 1084.215 kb on + strand, within moeB at 1084.216 kb on - strand, within moeB at 1084.216 kb on - strand, within moeB at 1084.279 kb on - strand, within moeB at 1084.307 kb on - strand, within moeB at 1084.373 kb on + strand, within moeB at 1084.487 kb on - strand at 1084.487 kb on - strand at 1084.580 kb on + strand at 1084.718 kb on - strand at 1084.816 kb on + strand, within fsa at 1084.955 kb on + strand, within fsa at 1085.000 kb on - strand, within fsa at 1085.023 kb on - strand, within fsa at 1085.023 kb on - strand, within fsa at 1085.114 kb on - strand, within fsa at 1085.168 kb on - strand, within fsa at 1085.171 kb on - strand, within fsa at 1085.321 kb on - strand, within fsa at 1085.435 kb on + strand at 1085.435 kb on + strand at 1085.435 kb on + strand at 1085.436 kb on - strand at 1085.436 kb on - strand at 1085.441 kb on - strand at 1085.531 kb on + strand at 1085.532 kb on - strand at 1085.754 kb on - strand, within ybiY at 1085.754 kb on - strand, within ybiY at 1086.052 kb on - strand, within ybiY at 1086.319 kb on + strand, within ybiY at 1086.320 kb on - strand, within ybiY at 1086.323 kb on + strand, within ybiY
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,083,777 + +0.5 1,083,845 - +0.2 1,084,050 + moeB OKFHMN_06120 0.32 +0.5 1,084,051 - moeB OKFHMN_06120 0.32 +0.8 1,084,051 - moeB OKFHMN_06120 0.32 -3.3 1,084,194 + moeB OKFHMN_06120 0.51 +0.1 1,084,215 + moeB OKFHMN_06120 0.54 +0.2 1,084,216 - moeB OKFHMN_06120 0.54 +2.3 1,084,216 - moeB OKFHMN_06120 0.54 +0.4 1,084,279 - moeB OKFHMN_06120 0.62 -0.3 1,084,307 - moeB OKFHMN_06120 0.66 -1.8 1,084,373 + moeB OKFHMN_06120 0.75 -1.6 1,084,487 - -0.1 1,084,487 - +1.0 1,084,580 + +0.8 1,084,718 - +0.4 1,084,816 + fsa OKFHMN_06130 0.12 +0.5 1,084,955 + fsa OKFHMN_06130 0.33 +0.2 1,085,000 - fsa OKFHMN_06130 0.39 +1.6 1,085,023 - fsa OKFHMN_06130 0.43 +0.6 1,085,023 - fsa OKFHMN_06130 0.43 +0.3 1,085,114 - fsa OKFHMN_06130 0.57 -1.1 1,085,168 - fsa OKFHMN_06130 0.65 -3.8 1,085,171 - fsa OKFHMN_06130 0.65 -0.3 1,085,321 - fsa OKFHMN_06130 0.88 -0.6 1,085,435 + +1.0 1,085,435 + -2.0 1,085,435 + -0.7 1,085,436 - +0.4 1,085,436 - -0.3 1,085,441 - +1.8 1,085,531 + -0.8 1,085,532 - +0.0 1,085,754 - ybiY OKFHMN_06135 0.25 -0.6 1,085,754 - ybiY OKFHMN_06135 0.25 +1.4 1,086,052 - ybiY OKFHMN_06135 0.58 -0.5 1,086,319 + ybiY OKFHMN_06135 0.87 +0.5 1,086,320 - ybiY OKFHMN_06135 0.88 +0.1 1,086,323 + ybiY OKFHMN_06135 0.88 +0.0
Or see this region's nucleotide sequence