Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKFHMN_05325 and elfD are separated by 24 nucleotides elfD and elfA are separated by 82 nucleotides elfA and ssuE are separated by 355 nucleotides ssuE and ssuA overlap by 8 nucleotides
OKFHMN_05325: OKFHMN_05325 - PapC-N domain-containing protein, at 886,878 to 887,480
_05325
OKFHMN_05330: elfD - putative fimbrial chaperone protein ElfD, at 887,505 to 888,206
elfD
OKFHMN_05335: elfA - Fimbrial subunit ElfA, at 888,289 to 888,828
elfA
OKFHMN_05340: ssuE - NADPH-dependent FMN reductase, at 889,184 to 889,759
ssuE
OKFHMN_05345: ssuA - aliphatic sulfonate ABC transporter substrate-binding protein SsuA, at 889,752 to 890,711
ssuA
Position (kb) 888
889 Strain fitness (log2 ratio) -2
-1
0
1
2 at 887.475 kb on - strand at 887.477 kb on - strand at 887.492 kb on - strand at 887.542 kb on + strand at 887.820 kb on - strand, within elfD at 887.916 kb on - strand, within elfD at 888.266 kb on - strand at 888.478 kb on - strand, within elfA at 888.526 kb on - strand, within elfA at 889.183 kb on + strand at 889.183 kb on + strand at 889.248 kb on - strand, within ssuE at 889.263 kb on + strand, within ssuE at 889.412 kb on + strand, within ssuE at 889.414 kb on + strand, within ssuE at 889.414 kb on + strand, within ssuE at 889.538 kb on + strand, within ssuE at 889.767 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 887,475 - +0.6 887,477 - +2.0 887,492 - +0.2 887,542 + -2.0 887,820 - elfD OKFHMN_05330 0.45 +1.4 887,916 - elfD OKFHMN_05330 0.59 +0.1 888,266 - +0.1 888,478 - elfA OKFHMN_05335 0.35 +0.2 888,526 - elfA OKFHMN_05335 0.44 +1.1 889,183 + +0.0 889,183 + -0.5 889,248 - ssuE OKFHMN_05340 0.11 +0.2 889,263 + ssuE OKFHMN_05340 0.14 +1.4 889,412 + ssuE OKFHMN_05340 0.40 -1.0 889,414 + ssuE OKFHMN_05340 0.40 -1.3 889,414 + ssuE OKFHMN_05340 0.40 -1.6 889,538 + ssuE OKFHMN_05340 0.61 -0.5 889,767 - +1.9
Or see this region's nucleotide sequence