Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKFHMN_05090 and OKFHMN_05095 are separated by 206 nucleotides OKFHMN_05095 and OKFHMN_05100 overlap by 17 nucleotides OKFHMN_05100 and hipB are separated by 108 nucleotides hipB and OKFHMN_05110 are separated by 27 nucleotides
OKFHMN_05090: OKFHMN_05090 - hypothetical protein, at 846,354 to 846,809
_05090
OKFHMN_05095: OKFHMN_05095 - Rha family transcriptional regulator, at 847,016 to 847,441
_05095
OKFHMN_05100: OKFHMN_05100 - Putative antitoxin of bacterial toxin-antitoxin system, YdaS/YdaT, at 847,425 to 847,697
_05100
OKFHMN_05105: hipB - XRE family transcriptional regulator, at 847,806 to 848,207
hipB
OKFHMN_05110: OKFHMN_05110 - antitoxin, at 848,235 to 848,426
_05110
Position (kb) 847
848 Strain fitness (log2 ratio) -2
-1
0
1 at 846.487 kb on + strand, within OKFHMN_05090 at 846.807 kb on + strand at 846.996 kb on + strand at 847.102 kb on + strand, within OKFHMN_05095 at 847.130 kb on + strand, within OKFHMN_05095 at 847.475 kb on + strand, within OKFHMN_05100 at 847.634 kb on + strand, within OKFHMN_05100 at 847.717 kb on + strand at 848.390 kb on + strand, within OKFHMN_05110 at 848.478 kb on + strand at 848.483 kb on + strand at 848.483 kb on + strand at 848.515 kb on + strand at 848.547 kb on + strand at 848.547 kb on + strand at 848.568 kb on - strand at 848.571 kb on + strand at 848.571 kb on + strand at 848.571 kb on + strand at 848.627 kb on + strand at 848.631 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 846,487 + OKFHMN_05090 0.29 -0.0 846,807 + -0.5 846,996 + -1.4 847,102 + OKFHMN_05095 0.20 +0.5 847,130 + OKFHMN_05095 0.27 -0.3 847,475 + OKFHMN_05100 0.18 -0.9 847,634 + OKFHMN_05100 0.77 -0.2 847,717 + -0.3 848,390 + OKFHMN_05110 0.81 -2.3 848,478 + +0.5 848,483 + -0.2 848,483 + -0.9 848,515 + +0.7 848,547 + -0.6 848,547 + -0.1 848,568 - +1.2 848,571 + +0.2 848,571 + +0.3 848,571 + -0.0 848,627 + +0.1 848,631 + -0.2
Or see this region's nucleotide sequence