Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybbA and OKFHMN_05070 are separated by 351 nucleotides OKFHMN_05070 and OKFHMN_05075 are separated by 15 nucleotides OKFHMN_05075 and dnaC are separated by 21 nucleotides dnaC and OKFHMN_05085 are separated by 6 nucleotides
OKFHMN_05065: ybbA - iroE, at 842,085 to 842,858
ybbA
OKFHMN_05070: OKFHMN_05070 - DUF977 domain-containing protein, at 843,210 to 843,623
_05070
OKFHMN_05075: OKFHMN_05075 - DUF1627 domain-containing protein, at 843,639 to 844,409
_05075
OKFHMN_05080: dnaC - DNA replication protein DnaC, at 844,431 to 845,177
dnaC
OKFHMN_05085: OKFHMN_05085 - DNA-binding protein, at 845,184 to 846,275
_05085
Position (kb) 843
844
845 Strain fitness (log2 ratio) -2
-1
0
1
2 at 843.152 kb on - strand at 843.313 kb on - strand, within OKFHMN_05070 at 843.613 kb on - strand at 843.935 kb on + strand, within OKFHMN_05075 at 843.984 kb on - strand, within OKFHMN_05075 at 844.194 kb on - strand, within OKFHMN_05075 at 844.294 kb on - strand, within OKFHMN_05075 at 844.482 kb on - strand at 844.615 kb on - strand, within dnaC at 844.627 kb on - strand, within dnaC at 844.812 kb on - strand, within dnaC at 844.952 kb on - strand, within dnaC at 845.142 kb on - strand at 845.363 kb on - strand, within OKFHMN_05085
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 843,152 - -1.0 843,313 - OKFHMN_05070 0.25 -1.0 843,613 - -0.3 843,935 + OKFHMN_05075 0.38 +1.8 843,984 - OKFHMN_05075 0.45 +0.3 844,194 - OKFHMN_05075 0.72 +0.3 844,294 - OKFHMN_05075 0.85 -1.9 844,482 - +1.3 844,615 - dnaC OKFHMN_05080 0.25 -0.8 844,627 - dnaC OKFHMN_05080 0.26 -1.4 844,812 - dnaC OKFHMN_05080 0.51 -0.1 844,952 - dnaC OKFHMN_05080 0.70 +0.1 845,142 - +0.1 845,363 - OKFHMN_05085 0.16 +0.6
Or see this region's nucleotide sequence