Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKFHMN_04785 and OKFHMN_04790 overlap by 4 nucleotides OKFHMN_04790 and hyaD overlap by 4 nucleotides hyaD and hyaC overlap by 4 nucleotides hyaC and hyaB are separated by 18 nucleotides
OKFHMN_04785: OKFHMN_04785 - hydrogenase expression/formation protein, at 799,300 to 800,157
_04785
OKFHMN_04790: OKFHMN_04790 - hydrogenase 1 maturation protease, at 800,154 to 800,552
_04790
OKFHMN_04795: hyaD - hydrogenase 1 maturation protease, at 800,549 to 801,136
hyaD
OKFHMN_04800: hyaC - Ni/Fe-hydrogenase b-type cytochrome subunit, at 801,133 to 801,840
hyaC
OKFHMN_04805: hyaB - Ni/Fe-hydrogenase large subunit, at 801,859 to 803,652
hyaB
Position (kb) 800
801
802 Strain fitness (log2 ratio) -1
0
1
2 at 799.979 kb on - strand, within OKFHMN_04785 at 799.979 kb on - strand, within OKFHMN_04785 at 799.990 kb on + strand, within OKFHMN_04785 at 800.226 kb on - strand, within OKFHMN_04790 at 800.431 kb on - strand, within OKFHMN_04790 at 800.459 kb on + strand, within OKFHMN_04790 at 800.625 kb on + strand, within hyaD at 800.625 kb on + strand, within hyaD at 800.789 kb on - strand, within hyaD at 801.049 kb on - strand, within hyaD at 801.243 kb on - strand, within hyaC at 801.243 kb on - strand, within hyaC at 801.372 kb on - strand, within hyaC at 801.473 kb on - strand, within hyaC at 801.507 kb on - strand, within hyaC at 801.668 kb on + strand, within hyaC at 801.668 kb on + strand, within hyaC at 801.669 kb on - strand, within hyaC at 801.676 kb on - strand, within hyaC at 801.676 kb on - strand, within hyaC at 801.677 kb on + strand, within hyaC at 801.678 kb on - strand, within hyaC at 801.686 kb on + strand, within hyaC at 801.687 kb on - strand, within hyaC at 801.784 kb on - strand at 801.948 kb on + strand at 802.077 kb on - strand, within hyaB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 799,979 - OKFHMN_04785 0.79 -0.2 799,979 - OKFHMN_04785 0.79 -0.5 799,990 + OKFHMN_04785 0.80 -0.0 800,226 - OKFHMN_04790 0.18 -1.3 800,431 - OKFHMN_04790 0.69 -0.3 800,459 + OKFHMN_04790 0.76 +1.1 800,625 + hyaD OKFHMN_04795 0.13 +0.1 800,625 + hyaD OKFHMN_04795 0.13 +0.4 800,789 - hyaD OKFHMN_04795 0.41 +0.4 801,049 - hyaD OKFHMN_04795 0.85 +1.2 801,243 - hyaC OKFHMN_04800 0.16 -0.6 801,243 - hyaC OKFHMN_04800 0.16 -0.3 801,372 - hyaC OKFHMN_04800 0.34 +1.8 801,473 - hyaC OKFHMN_04800 0.48 +0.0 801,507 - hyaC OKFHMN_04800 0.53 -0.8 801,668 + hyaC OKFHMN_04800 0.76 +0.6 801,668 + hyaC OKFHMN_04800 0.76 +0.3 801,669 - hyaC OKFHMN_04800 0.76 +0.8 801,676 - hyaC OKFHMN_04800 0.77 -0.3 801,676 - hyaC OKFHMN_04800 0.77 +0.8 801,677 + hyaC OKFHMN_04800 0.77 -0.8 801,678 - hyaC OKFHMN_04800 0.77 -1.4 801,686 + hyaC OKFHMN_04800 0.78 +0.9 801,687 - hyaC OKFHMN_04800 0.78 +0.3 801,784 - +2.0 801,948 + -0.1 802,077 - hyaB OKFHMN_04805 0.12 -0.1
Or see this region's nucleotide sequence