Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKFHMN_02725 and OKFHMN_02730 are separated by 284 nucleotides OKFHMN_02730 and iS285 are separated by 29 nucleotides iS285 and OKFHMN_02740 are separated by 255 nucleotides OKFHMN_02740 and nleH1-2 are separated by 65 nucleotides
OKFHMN_02725: OKFHMN_02725 - IS66-like element ISEc8 family transposase, at 436,172 to 437,710
_02725
OKFHMN_02730: OKFHMN_02730 - Mutator family transposase, at 437,995 to 438,342
_02730
OKFHMN_02735: iS285 - Transposase (or an inactivated derivative), at 438,372 to 438,629
iS285
OKFHMN_02740: OKFHMN_02740 - T3SS secreted effector NleF, at 438,885 to 439,244
_02740
OKFHMN_02745: nleH1-2 - type III secretion system effector kinase NleH1-2, at 439,310 to 440,221
nleH1-2
Position (kb) 438
439 Strain fitness (log2 ratio) -2
-1
0
1
2 at 438.002 kb on + strand at 438.179 kb on + strand, within OKFHMN_02730 at 438.341 kb on - strand at 438.369 kb on - strand at 438.417 kb on + strand, within iS285 at 438.418 kb on - strand, within iS285 at 438.591 kb on - strand, within iS285 at 438.689 kb on - strand at 438.705 kb on - strand at 438.742 kb on - strand at 438.797 kb on - strand at 438.921 kb on - strand, within OKFHMN_02740 at 439.101 kb on - strand, within OKFHMN_02740
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 438,002 + +1.2 438,179 + OKFHMN_02730 0.53 +0.3 438,341 - -1.0 438,369 - +0.6 438,417 + iS285 OKFHMN_02735 0.17 -0.1 438,418 - iS285 OKFHMN_02735 0.18 -1.1 438,591 - iS285 OKFHMN_02735 0.85 -1.7 438,689 - +0.2 438,705 - -2.5 438,742 - +0.6 438,797 - +1.4 438,921 - OKFHMN_02740 0.10 +2.0 439,101 - OKFHMN_02740 0.60 -1.2
Or see this region's nucleotide sequence