Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pgpB and ribA are separated by 169 nucleotides ribA and acnA are separated by 63 nucleotides acnA and ymiC are separated by 163 nucleotides ymiC and OKFHMN_02590 are separated by 128 nucleotides OKFHMN_02590 and ymiA are separated by 2 nucleotides
OKFHMN_02565: pgpB - phosphatidylglycerophosphatase B, at 404,114 to 404,878
pgpB
OKFHMN_02570: ribA - GTP cyclohydrolase-2, at 405,048 to 405,638
ribA
OKFHMN_02575: acnA - aconitate hydratase AcnA, at 405,702 to 408,377
acnA
OKFHMN_02585: ymiC - Protein YmiC, at 408,541 to 408,621
ymiC
OKFHMN_02590: OKFHMN_02590 - Protein, C-ter, truncated protein, at 408,750 to 408,917
_02590
OKFHMN_02595: ymiA - YmiA family putative membrane protein, at 408,920 to 409,048
ymiA
Position (kb) 405
406
407
408
409 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 404.896 kb on - strand at 404.910 kb on + strand at 405.637 kb on - strand at 405.714 kb on - strand at 405.727 kb on + strand at 406.131 kb on - strand, within acnA at 406.350 kb on - strand, within acnA at 406.462 kb on + strand, within acnA at 406.463 kb on - strand, within acnA at 406.487 kb on - strand, within acnA at 406.630 kb on + strand, within acnA at 406.739 kb on + strand, within acnA at 406.875 kb on - strand, within acnA at 407.290 kb on - strand, within acnA at 407.402 kb on - strand, within acnA at 407.402 kb on - strand, within acnA at 407.463 kb on - strand, within acnA at 407.767 kb on + strand, within acnA at 408.213 kb on - strand at 408.310 kb on + strand at 408.641 kb on + strand at 408.704 kb on - strand at 408.811 kb on - strand, within OKFHMN_02590 at 408.918 kb on - strand at 408.918 kb on - strand at 408.918 kb on - strand at 408.918 kb on - strand at 409.061 kb on - strand at 409.063 kb on - strand at 409.104 kb on - strand at 409.242 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 404,896 - +1.6 404,910 + +0.6 405,637 - +0.1 405,714 - +1.0 405,727 + +0.1 406,131 - acnA OKFHMN_02575 0.16 -2.5 406,350 - acnA OKFHMN_02575 0.24 -0.1 406,462 + acnA OKFHMN_02575 0.28 -1.5 406,463 - acnA OKFHMN_02575 0.28 +2.2 406,487 - acnA OKFHMN_02575 0.29 -4.0 406,630 + acnA OKFHMN_02575 0.35 -0.4 406,739 + acnA OKFHMN_02575 0.39 +0.7 406,875 - acnA OKFHMN_02575 0.44 +0.1 407,290 - acnA OKFHMN_02575 0.59 -1.5 407,402 - acnA OKFHMN_02575 0.64 -0.7 407,402 - acnA OKFHMN_02575 0.64 -0.3 407,463 - acnA OKFHMN_02575 0.66 +0.6 407,767 + acnA OKFHMN_02575 0.77 -2.1 408,213 - -0.9 408,310 + -0.7 408,641 + -0.2 408,704 - -0.3 408,811 - OKFHMN_02590 0.36 -2.9 408,918 - -0.2 408,918 - +0.8 408,918 - +0.0 408,918 - -1.0 409,061 - -0.1 409,063 - -0.3 409,104 - -1.3 409,242 + +0.1
Or see this region's nucleotide sequence