Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yciH and pyrF overlap by 1 nucleotides pyrF and lapB are separated by 132 nucleotides lapB and lapA are separated by 6 nucleotides lapA and pgpB are separated by 148 nucleotides
OKFHMN_02545: yciH - stress response translation initiation inhibitor YciH, at 401,225 to 401,551
yciH
OKFHMN_02550: pyrF - orotidine-5'-phosphate decarboxylase, at 401,551 to 402,348
pyrF
OKFHMN_02555: lapB - lipopolysaccharide assembly protein LapB, at 402,481 to 403,650
lapB
OKFHMN_02560: lapA - lipopolysaccharide assembly protein LapA, at 403,657 to 403,965
lapA
OKFHMN_02565: pgpB - phosphatidylglycerophosphatase B, at 404,114 to 404,878
pgpB
Position (kb) 402
403
404 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 401.567 kb on - strand at 401.637 kb on + strand, within pyrF at 401.638 kb on - strand, within pyrF at 401.840 kb on + strand, within pyrF at 402.017 kb on - strand, within pyrF at 403.865 kb on - strand, within lapA at 403.969 kb on - strand at 404.028 kb on - strand at 404.234 kb on - strand, within pgpB at 404.237 kb on + strand, within pgpB at 404.238 kb on - strand, within pgpB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 401,567 - -0.7 401,637 + pyrF OKFHMN_02550 0.11 -4.7 401,638 - pyrF OKFHMN_02550 0.11 -3.2 401,840 + pyrF OKFHMN_02550 0.36 -3.6 402,017 - pyrF OKFHMN_02550 0.58 -3.3 403,865 - lapA OKFHMN_02560 0.67 +0.9 403,969 - -1.5 404,028 - +0.1 404,234 - pgpB OKFHMN_02565 0.16 +0.5 404,237 + pgpB OKFHMN_02565 0.16 +0.1 404,238 - pgpB OKFHMN_02565 0.16 -0.3
Or see this region's nucleotide sequence