Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02540

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyciT and osmB are separated by 268 nucleotidesosmB and yciH are separated by 125 nucleotidesyciH and pyrF overlap by 1 nucleotides OKFHMN_02535: yciT - DNA-binding transcriptional regulator YciT, at 399,863 to 400,612 yciT OKFHMN_02540: osmB - osmotically-inducible lipoprotein OsmB, at 400,881 to 401,099 osmB OKFHMN_02545: yciH - stress response translation initiation inhibitor YciH, at 401,225 to 401,551 yciH OKFHMN_02550: pyrF - orotidine-5'-phosphate decarboxylase, at 401,551 to 402,348 pyrF Position (kb) 400 401 402Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 400.070 kb on + strand, within yciTat 400.663 kb on - strandat 400.826 kb on + strandat 401.567 kb on - strandat 401.637 kb on + strand, within pyrFat 401.638 kb on - strand, within pyrFat 401.840 kb on + strand, within pyrFat 402.017 kb on - strand, within pyrF

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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400,070 + yciT OKFHMN_02535 0.28 -2.9
400,663 - -2.1
400,826 + -1.4
401,567 - -0.7
401,637 + pyrF OKFHMN_02550 0.11 -4.7
401,638 - pyrF OKFHMN_02550 0.11 -3.2
401,840 + pyrF OKFHMN_02550 0.36 -3.6
402,017 - pyrF OKFHMN_02550 0.58 -3.3

Or see this region's nucleotide sequence