Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02240

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntabgT and ogt are separated by 10 nucleotidesogt and fnr are separated by 194 nucleotidesfnr and uspE are separated by 151 nucleotides OKFHMN_02230: abgT - p-aminobenzoyl-glutamate transporter, at 335,818 to 336,510 abgT OKFHMN_02235: ogt - methylated-DNA--[protein]-cysteine S-methyltransferase, at 336,521 to 337,036 ogt OKFHMN_02240: fnr - fumarate/nitrate reduction transcriptional regulator Fnr, at 337,231 to 337,983 fnr OKFHMN_02245: uspE - universal stress protein UspE, at 338,135 to 339,085 uspE Position (kb) 337 338Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 336.249 kb on - strand, within abgTat 336.392 kb on - strand, within abgTat 336.748 kb on + strand, within ogtat 336.748 kb on + strand, within ogtat 337.165 kb on + strandat 337.230 kb on + strandat 337.230 kb on + strandat 337.251 kb on + strandat 337.383 kb on + strand, within fnrat 337.467 kb on + strand, within fnrat 337.753 kb on + strand, within fnrat 337.753 kb on + strand, within fnrat 337.833 kb on + strand, within fnrat 337.837 kb on + strand, within fnrat 337.995 kb on - strandat 338.078 kb on + strandat 338.140 kb on + strandat 338.305 kb on + strand, within uspEat 338.370 kb on - strand, within uspEat 338.917 kb on - strand, within uspE

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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336,249 - abgT OKFHMN_02230 0.62 +0.6
336,392 - abgT OKFHMN_02230 0.83 +0.2
336,748 + ogt OKFHMN_02235 0.44 -3.3
336,748 + ogt OKFHMN_02235 0.44 +3.1
337,165 + -0.0
337,230 + -0.9
337,230 + -1.7
337,251 + +0.3
337,383 + fnr OKFHMN_02240 0.20 -0.2
337,467 + fnr OKFHMN_02240 0.31 +1.6
337,753 + fnr OKFHMN_02240 0.69 -0.6
337,753 + fnr OKFHMN_02240 0.69 -2.7
337,833 + fnr OKFHMN_02240 0.80 +0.2
337,837 + fnr OKFHMN_02240 0.80 +2.0
337,995 - +0.1
338,078 + +0.3
338,140 + +0.9
338,305 + uspE OKFHMN_02245 0.18 +0.9
338,370 - uspE OKFHMN_02245 0.25 -2.3
338,917 - uspE OKFHMN_02245 0.82 +1.6

Or see this region's nucleotide sequence