Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydaV and OKFHMN_02070 are separated by 6 nucleotides OKFHMN_02070 and ydaT are separated by 77 nucleotides ydaT and OKFHMN_02080 overlap by 4 nucleotides OKFHMN_02080 and OKFHMN_02085 are separated by 96 nucleotides
OKFHMN_02065: ydaV - Uncharacterized protein YdaV, at 313,192 to 313,938
ydaV
OKFHMN_02070: OKFHMN_02070 - Putative DNA-binding phage protein, at 313,945 to 314,733
_02070
OKFHMN_02075: ydaT - Uncharacterized protein YdaT, at 314,811 to 315,233
ydaT
OKFHMN_02080: OKFHMN_02080 - transcriptional regulator, at 315,230 to 315,472
_02080
OKFHMN_02085: OKFHMN_02085 - transcriptional regulator, at 315,569 to 315,988
_02085
Position (kb) 314
315
316 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 313.877 kb on - strand at 314.092 kb on - strand, within OKFHMN_02070 at 314.232 kb on + strand, within OKFHMN_02070 at 314.571 kb on + strand, within OKFHMN_02070 at 314.749 kb on - strand at 314.749 kb on - strand at 315.139 kb on + strand, within ydaT at 315.248 kb on + strand at 315.248 kb on + strand at 315.326 kb on - strand, within OKFHMN_02080 at 315.407 kb on + strand, within OKFHMN_02080 at 315.407 kb on + strand, within OKFHMN_02080 at 315.970 kb on + strand at 316.114 kb on + strand at 316.157 kb on + strand at 316.158 kb on - strand at 316.188 kb on + strand at 316.208 kb on + strand at 316.208 kb on + strand at 316.208 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 313,877 - -0.9 314,092 - OKFHMN_02070 0.19 +0.7 314,232 + OKFHMN_02070 0.36 +0.2 314,571 + OKFHMN_02070 0.79 -1.2 314,749 - -3.2 314,749 - -2.9 315,139 + ydaT OKFHMN_02075 0.78 -0.1 315,248 + -0.8 315,248 + +0.3 315,326 - OKFHMN_02080 0.40 -2.9 315,407 + OKFHMN_02080 0.73 -4.1 315,407 + OKFHMN_02080 0.73 -0.8 315,970 + -0.8 316,114 + +0.3 316,157 + -0.2 316,158 - +0.8 316,188 + -0.0 316,208 + -0.4 316,208 + +0.4 316,208 + +0.5
Or see this region's nucleotide sequence