Strain Fitness in Escherichia coli ECRC100 around OKFHMN_01445

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyddE and OKFHMN_01445 are separated by 95 nucleotidesOKFHMN_01445 and nhoA overlap by 1 nucleotidesnhoA and yddH are separated by 172 nucleotides OKFHMN_01440: yddE - PhzF family isomerase, at 195,963 to 196,856 yddE OKFHMN_01445: OKFHMN_01445 - N-hydroxyarylamine O-acetyltransferase, at 196,952 to 197,062 _01445 OKFHMN_01450: nhoA - N-hydroxyarylamine O-acetyltransferase, at 197,062 to 197,796 nhoA OKFHMN_01455: yddH - Uncharacterized protein YddH, at 197,969 to 198,091 yddH Position (kb) 196 197 198Strain fitness (log2 ratio) -2 -1 0 1at 196.207 kb on + strand, within yddEat 196.672 kb on + strand, within yddEat 196.672 kb on + strand, within yddEat 197.000 kb on + strand, within OKFHMN_01445at 197.071 kb on - strandat 197.247 kb on - strand, within nhoAat 197.390 kb on - strand, within nhoAat 197.515 kb on + strand, within nhoAat 197.827 kb on + strandat 197.935 kb on - strandat 197.935 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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196,207 + yddE OKFHMN_01440 0.27 -0.7
196,672 + yddE OKFHMN_01440 0.79 -0.1
196,672 + yddE OKFHMN_01440 0.79 +1.6
197,000 + OKFHMN_01445 0.43 +0.7
197,071 - +0.7
197,247 - nhoA OKFHMN_01450 0.25 +0.8
197,390 - nhoA OKFHMN_01450 0.45 +0.9
197,515 + nhoA OKFHMN_01450 0.62 -0.5
197,827 + -2.5
197,935 - -0.5
197,935 - -0.3

Or see this region's nucleotide sequence