Strain Fitness in Escherichia coli ECRC100 around OKFHMN_01150

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttam and lsrG are separated by 56 nucleotideslsrG and lsrF are separated by 23 nucleotideslsrF and lsrB are separated by 26 nucleotides OKFHMN_01140: tam - trans-aconitate 2-methyltransferase, at 116,580 to 117,338 tam OKFHMN_01145: lsrG - (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase, at 117,395 to 117,685 lsrG OKFHMN_01150: lsrF - 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase, at 117,709 to 118,584 lsrF OKFHMN_01155: lsrB - autoinducer 2 ABC transporter substrate-binding protein LsrB, at 118,611 to 119,633 lsrB Position (kb) 117 118 119Strain fitness (log2 ratio) -2 -1 0 1at 117.313 kb on + strandat 117.315 kb on + strandat 117.491 kb on - strand, within lsrGat 117.729 kb on - strandat 117.738 kb on - strandat 118.738 kb on - strand, within lsrBat 118.738 kb on - strand, within lsrBat 119.014 kb on - strand, within lsrB

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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117,313 + -0.3
117,315 + -0.2
117,491 - lsrG OKFHMN_01145 0.33 +0.2
117,729 - -0.4
117,738 - -2.5
118,738 - lsrB OKFHMN_01155 0.12 +0.2
118,738 - lsrB OKFHMN_01155 0.12 +0.8
119,014 - lsrB OKFHMN_01155 0.39 -0.0

Or see this region's nucleotide sequence