Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspK and gspJ overlap by 4 nucleotides gspJ and gspI overlap by 4 nucleotides gspI and gspH overlap by 4 nucleotides gspH and gspG overlap by 4 nucleotides gspG and gspF are separated by 30 nucleotides
OKFHMN_00250: gspK - type II secretion system minor pseudopilin GspK, at 52,066 to 53,043
gspK
OKFHMN_00255: gspJ - type II secretion system minor pseudopilin GspJ, at 53,040 to 53,639
gspJ
OKFHMN_00260: gspI - type II secretion system minor pseudopilin GspI, at 53,636 to 54,001
gspI
OKFHMN_00265: gspH - type II secretion system minor pseudopilin GspH, at 53,998 to 54,552
gspH
OKFHMN_00270: gspG - type II secretion system major pseudopilin GspG, at 54,549 to 54,983
gspG
OKFHMN_00275: gspF - type II secretion system inner membrane protein GspF, at 55,014 to 56,237
gspF
Position (kb) 53
54
55 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 53.005 kb on + strand at 53.272 kb on - strand, within gspJ at 53.298 kb on + strand, within gspJ at 53.299 kb on - strand, within gspJ at 53.299 kb on - strand, within gspJ at 53.299 kb on - strand, within gspJ at 53.307 kb on + strand, within gspJ at 53.307 kb on + strand, within gspJ at 53.308 kb on - strand, within gspJ at 53.308 kb on - strand, within gspJ at 53.451 kb on - strand, within gspJ at 53.502 kb on + strand, within gspJ at 53.502 kb on + strand, within gspJ at 53.503 kb on - strand, within gspJ at 53.503 kb on - strand, within gspJ at 53.503 kb on - strand, within gspJ at 53.508 kb on - strand, within gspJ at 53.532 kb on + strand, within gspJ at 53.612 kb on + strand at 53.613 kb on - strand at 53.613 kb on - strand at 53.622 kb on + strand at 53.623 kb on - strand at 53.656 kb on + strand at 53.689 kb on + strand, within gspI at 53.699 kb on + strand, within gspI at 53.699 kb on + strand, within gspI at 53.699 kb on + strand, within gspI at 53.705 kb on + strand, within gspI at 53.706 kb on - strand, within gspI at 53.706 kb on - strand, within gspI at 53.756 kb on + strand, within gspI at 53.756 kb on + strand, within gspI at 53.757 kb on - strand, within gspI at 53.757 kb on - strand, within gspI at 53.960 kb on + strand, within gspI at 53.960 kb on + strand, within gspI at 53.961 kb on - strand, within gspI at 53.961 kb on - strand, within gspI at 53.961 kb on - strand, within gspI at 53.963 kb on + strand, within gspI at 53.964 kb on - strand, within gspI at 53.964 kb on - strand, within gspI at 53.964 kb on - strand, within gspI at 54.185 kb on + strand, within gspH at 54.263 kb on + strand, within gspH at 54.263 kb on + strand, within gspH at 54.264 kb on - strand, within gspH at 54.264 kb on - strand, within gspH at 54.264 kb on - strand, within gspH at 54.277 kb on - strand, within gspH at 54.293 kb on - strand, within gspH at 54.293 kb on - strand, within gspH at 54.333 kb on + strand, within gspH at 54.393 kb on + strand, within gspH at 54.393 kb on + strand, within gspH at 54.393 kb on + strand, within gspH at 54.394 kb on - strand, within gspH at 54.404 kb on + strand, within gspH at 54.405 kb on - strand, within gspH at 54.475 kb on + strand, within gspH at 54.475 kb on + strand, within gspH at 54.475 kb on + strand, within gspH at 54.475 kb on + strand, within gspH at 54.475 kb on + strand, within gspH at 54.475 kb on + strand, within gspH at 54.476 kb on - strand, within gspH at 54.476 kb on - strand, within gspH at 54.476 kb on - strand, within gspH at 54.479 kb on - strand, within gspH at 54.585 kb on - strand at 54.629 kb on + strand, within gspG at 54.644 kb on - strand, within gspG at 54.644 kb on - strand, within gspG at 54.658 kb on - strand, within gspG at 54.667 kb on + strand, within gspG at 54.668 kb on - strand, within gspG at 54.704 kb on + strand, within gspG at 54.704 kb on + strand, within gspG at 54.721 kb on + strand, within gspG at 54.801 kb on - strand, within gspG at 54.811 kb on + strand, within gspG at 54.811 kb on + strand, within gspG at 54.812 kb on - strand, within gspG at 54.878 kb on + strand, within gspG at 54.878 kb on + strand, within gspG at 54.879 kb on - strand, within gspG at 54.933 kb on + strand, within gspG at 54.933 kb on + strand, within gspG at 54.943 kb on - strand at 54.956 kb on + strand at 54.956 kb on + strand at 54.957 kb on - strand at 54.957 kb on - strand at 55.003 kb on + strand at 55.004 kb on - strand at 55.019 kb on + strand at 55.020 kb on - strand at 55.020 kb on - strand at 55.071 kb on - strand at 55.235 kb on + strand, within gspF at 55.307 kb on + strand, within gspF at 55.311 kb on + strand, within gspF at 55.391 kb on + strand, within gspF at 55.392 kb on - strand, within gspF at 55.414 kb on + strand, within gspF at 55.414 kb on + strand, within gspF at 55.415 kb on - strand, within gspF at 55.415 kb on - strand, within gspF at 55.506 kb on + strand, within gspF at 55.506 kb on + strand, within gspF at 55.506 kb on + strand, within gspF at 55.507 kb on - strand, within gspF at 55.509 kb on + strand, within gspF at 55.509 kb on + strand, within gspF at 55.548 kb on + strand, within gspF at 55.549 kb on - strand, within gspF
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 53,005 + +0.2 53,272 - gspJ OKFHMN_00255 0.39 -0.4 53,298 + gspJ OKFHMN_00255 0.43 +0.5 53,299 - gspJ OKFHMN_00255 0.43 +0.1 53,299 - gspJ OKFHMN_00255 0.43 +1.0 53,299 - gspJ OKFHMN_00255 0.43 +0.3 53,307 + gspJ OKFHMN_00255 0.45 -0.3 53,307 + gspJ OKFHMN_00255 0.45 -0.2 53,308 - gspJ OKFHMN_00255 0.45 +0.1 53,308 - gspJ OKFHMN_00255 0.45 -0.1 53,451 - gspJ OKFHMN_00255 0.69 +0.3 53,502 + gspJ OKFHMN_00255 0.77 -0.1 53,502 + gspJ OKFHMN_00255 0.77 -0.6 53,503 - gspJ OKFHMN_00255 0.77 -1.1 53,503 - gspJ OKFHMN_00255 0.77 +0.1 53,503 - gspJ OKFHMN_00255 0.77 +0.3 53,508 - gspJ OKFHMN_00255 0.78 -0.6 53,532 + gspJ OKFHMN_00255 0.82 +1.5 53,612 + -0.7 53,613 - -2.0 53,613 - -0.1 53,622 + -0.7 53,623 - +0.7 53,656 + +0.5 53,689 + gspI OKFHMN_00260 0.14 -0.0 53,699 + gspI OKFHMN_00260 0.17 +1.2 53,699 + gspI OKFHMN_00260 0.17 -0.3 53,699 + gspI OKFHMN_00260 0.17 +0.3 53,705 + gspI OKFHMN_00260 0.19 +0.8 53,706 - gspI OKFHMN_00260 0.19 -1.3 53,706 - gspI OKFHMN_00260 0.19 +0.1 53,756 + gspI OKFHMN_00260 0.33 +0.4 53,756 + gspI OKFHMN_00260 0.33 +1.7 53,757 - gspI OKFHMN_00260 0.33 -0.0 53,757 - gspI OKFHMN_00260 0.33 -0.2 53,960 + gspI OKFHMN_00260 0.89 -1.1 53,960 + gspI OKFHMN_00260 0.89 -0.5 53,961 - gspI OKFHMN_00260 0.89 -4.4 53,961 - gspI OKFHMN_00260 0.89 +3.3 53,961 - gspI OKFHMN_00260 0.89 +1.5 53,963 + gspI OKFHMN_00260 0.89 +0.9 53,964 - gspI OKFHMN_00260 0.90 -1.3 53,964 - gspI OKFHMN_00260 0.90 -1.6 53,964 - gspI OKFHMN_00260 0.90 -0.3 54,185 + gspH OKFHMN_00265 0.34 -0.3 54,263 + gspH OKFHMN_00265 0.48 +0.9 54,263 + gspH OKFHMN_00265 0.48 +1.1 54,264 - gspH OKFHMN_00265 0.48 -2.5 54,264 - gspH OKFHMN_00265 0.48 +0.8 54,264 - gspH OKFHMN_00265 0.48 -1.3 54,277 - gspH OKFHMN_00265 0.50 +0.7 54,293 - gspH OKFHMN_00265 0.53 -0.4 54,293 - gspH OKFHMN_00265 0.53 -0.3 54,333 + gspH OKFHMN_00265 0.60 +0.8 54,393 + gspH OKFHMN_00265 0.71 -1.4 54,393 + gspH OKFHMN_00265 0.71 -0.2 54,393 + gspH OKFHMN_00265 0.71 -0.6 54,394 - gspH OKFHMN_00265 0.71 -0.4 54,404 + gspH OKFHMN_00265 0.73 -0.2 54,405 - gspH OKFHMN_00265 0.73 -0.2 54,475 + gspH OKFHMN_00265 0.86 +0.4 54,475 + gspH OKFHMN_00265 0.86 -0.2 54,475 + gspH OKFHMN_00265 0.86 +0.1 54,475 + gspH OKFHMN_00265 0.86 +0.3 54,475 + gspH OKFHMN_00265 0.86 +0.8 54,475 + gspH OKFHMN_00265 0.86 -2.4 54,476 - gspH OKFHMN_00265 0.86 +0.6 54,476 - gspH OKFHMN_00265 0.86 +0.5 54,476 - gspH OKFHMN_00265 0.86 +0.1 54,479 - gspH OKFHMN_00265 0.87 +0.2 54,585 - +2.1 54,629 + gspG OKFHMN_00270 0.18 -0.9 54,644 - gspG OKFHMN_00270 0.22 +0.3 54,644 - gspG OKFHMN_00270 0.22 +1.2 54,658 - gspG OKFHMN_00270 0.25 -0.6 54,667 + gspG OKFHMN_00270 0.27 -0.3 54,668 - gspG OKFHMN_00270 0.27 -0.1 54,704 + gspG OKFHMN_00270 0.36 +0.5 54,704 + gspG OKFHMN_00270 0.36 -0.8 54,721 + gspG OKFHMN_00270 0.40 -0.3 54,801 - gspG OKFHMN_00270 0.58 +0.0 54,811 + gspG OKFHMN_00270 0.60 +0.8 54,811 + gspG OKFHMN_00270 0.60 -0.3 54,812 - gspG OKFHMN_00270 0.60 +0.7 54,878 + gspG OKFHMN_00270 0.76 -0.3 54,878 + gspG OKFHMN_00270 0.76 +0.2 54,879 - gspG OKFHMN_00270 0.76 +0.5 54,933 + gspG OKFHMN_00270 0.88 +0.1 54,933 + gspG OKFHMN_00270 0.88 +0.6 54,943 - -0.1 54,956 + +0.3 54,956 + +0.8 54,957 - +0.4 54,957 - +0.1 55,003 + -0.1 55,004 - -2.5 55,019 + -0.1 55,020 - -3.4 55,020 - +0.2 55,071 - -0.2 55,235 + gspF OKFHMN_00275 0.18 -0.1 55,307 + gspF OKFHMN_00275 0.24 -0.0 55,311 + gspF OKFHMN_00275 0.24 +0.1 55,391 + gspF OKFHMN_00275 0.31 -0.2 55,392 - gspF OKFHMN_00275 0.31 +0.6 55,414 + gspF OKFHMN_00275 0.33 -0.1 55,414 + gspF OKFHMN_00275 0.33 -0.4 55,415 - gspF OKFHMN_00275 0.33 +0.7 55,415 - gspF OKFHMN_00275 0.33 +1.1 55,506 + gspF OKFHMN_00275 0.40 +2.2 55,506 + gspF OKFHMN_00275 0.40 -1.3 55,506 + gspF OKFHMN_00275 0.40 -1.6 55,507 - gspF OKFHMN_00275 0.40 +0.9 55,509 + gspF OKFHMN_00275 0.40 -1.5 55,509 + gspF OKFHMN_00275 0.40 +0.8 55,548 + gspF OKFHMN_00275 0.44 -0.2 55,549 - gspF OKFHMN_00275 0.44 -3.5
Or see this region's nucleotide sequence