Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yubM and yubL are separated by 65 nucleotides yubL and ssb are separated by 56 nucleotides ssb and OKFHMN_00065 are separated by 594 nucleotides OKFHMN_00065 and rsmA overlap by 1 nucleotides
OKFHMN_00050: yubM - Uncharacterized protein YubM, at 15,894 to 17,852
yubM
OKFHMN_00055: yubL - UPF0401 protein YubL, at 17,918 to 18,151
yubL
OKFHMN_00060: ssb - single-stranded DNA-binding protein, at 18,208 to 18,660
ssb
OKFHMN_00065: OKFHMN_00065 - hypothetical protein, at 19,255 to 19,485
_00065
OKFHMN_00070: rsmA - SAM-dependent methyltransferase, at 19,485 to 20,048
rsmA
Position (kb)
18
19 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 17.445 kb on - strand, within yubM at 17.652 kb on - strand, within yubM at 17.720 kb on + strand at 17.721 kb on - strand at 17.721 kb on - strand at 17.721 kb on - strand at 17.721 kb on - strand at 17.721 kb on - strand at 17.816 kb on + strand at 17.817 kb on - strand at 17.852 kb on + strand at 17.852 kb on + strand at 17.852 kb on + strand at 17.852 kb on + strand at 17.852 kb on + strand at 17.856 kb on + strand at 17.856 kb on + strand at 17.856 kb on + strand at 17.856 kb on + strand at 17.856 kb on + strand at 17.856 kb on + strand at 17.856 kb on + strand at 17.856 kb on + strand at 17.856 kb on + strand at 17.856 kb on + strand at 17.856 kb on + strand at 17.856 kb on + strand at 17.856 kb on + strand at 17.856 kb on + strand at 17.857 kb on - strand at 17.857 kb on - strand at 17.857 kb on - strand at 17.857 kb on - strand at 17.857 kb on - strand at 17.857 kb on - strand at 17.857 kb on - strand at 17.857 kb on - strand at 17.857 kb on - strand at 17.859 kb on + strand at 17.859 kb on + strand at 17.859 kb on + strand at 17.860 kb on - strand at 17.875 kb on - strand at 17.877 kb on + strand at 17.919 kb on + strand at 17.923 kb on + strand at 17.923 kb on + strand at 17.923 kb on + strand at 17.923 kb on + strand at 17.923 kb on + strand at 17.923 kb on + strand at 17.923 kb on + strand at 17.923 kb on + strand at 17.924 kb on - strand at 17.926 kb on + strand at 17.926 kb on + strand at 17.927 kb on - strand at 18.010 kb on + strand, within yubL at 18.013 kb on + strand, within yubL at 18.014 kb on - strand, within yubL at 18.131 kb on + strand at 18.132 kb on - strand at 18.219 kb on + strand at 18.322 kb on + strand, within ssb at 18.322 kb on + strand, within ssb at 18.323 kb on - strand, within ssb at 18.381 kb on + strand, within ssb at 18.425 kb on + strand, within ssb at 18.449 kb on + strand, within ssb at 18.449 kb on + strand, within ssb at 18.594 kb on - strand, within ssb at 18.642 kb on + strand at 18.642 kb on + strand at 18.643 kb on - strand at 18.643 kb on - strand at 19.253 kb on - strand at 19.282 kb on + strand, within OKFHMN_00065 at 19.283 kb on - strand, within OKFHMN_00065 at 19.288 kb on + strand, within OKFHMN_00065 at 19.289 kb on - strand, within OKFHMN_00065 at 19.295 kb on - strand, within OKFHMN_00065 at 19.300 kb on + strand, within OKFHMN_00065 at 19.300 kb on + strand, within OKFHMN_00065 at 19.301 kb on - strand, within OKFHMN_00065 at 19.324 kb on + strand, within OKFHMN_00065 at 19.325 kb on - strand, within OKFHMN_00065 at 19.378 kb on + strand, within OKFHMN_00065 at 19.383 kb on + strand, within OKFHMN_00065 at 19.383 kb on + strand, within OKFHMN_00065 at 19.384 kb on - strand, within OKFHMN_00065 at 19.384 kb on - strand, within OKFHMN_00065 at 19.503 kb on - strand at 19.525 kb on + strand at 19.525 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 17,445 - yubM OKFHMN_00050 0.79 +0.1 17,652 - yubM OKFHMN_00050 0.90 +0.1 17,720 + -0.0 17,721 - -0.7 17,721 - -1.6 17,721 - -0.3 17,721 - -1.6 17,721 - -1.4 17,816 + -0.5 17,817 - -0.3 17,852 + -0.1 17,852 + -0.0 17,852 + +2.0 17,852 + +1.2 17,852 + -0.5 17,856 + -0.2 17,856 + -0.5 17,856 + -0.5 17,856 + +0.4 17,856 + -0.6 17,856 + -0.2 17,856 + -0.1 17,856 + -0.4 17,856 + -0.1 17,856 + -0.8 17,856 + -0.2 17,856 + -0.5 17,856 + -0.0 17,856 + -0.5 17,857 - +0.8 17,857 - -2.8 17,857 - -3.1 17,857 - -0.3 17,857 - +0.1 17,857 - -0.7 17,857 - -1.9 17,857 - -0.7 17,857 - -0.8 17,859 + +0.5 17,859 + +0.1 17,859 + +0.5 17,860 - +0.6 17,875 - +0.9 17,877 + +0.9 17,919 + -0.4 17,923 + +0.1 17,923 + +2.5 17,923 + +1.1 17,923 + -0.0 17,923 + -0.3 17,923 + +0.3 17,923 + -0.4 17,923 + +1.2 17,924 - -2.5 17,926 + +0.5 17,926 + -0.2 17,927 - -1.6 18,010 + yubL OKFHMN_00055 0.39 +0.6 18,013 + yubL OKFHMN_00055 0.41 +0.4 18,014 - yubL OKFHMN_00055 0.41 -0.4 18,131 + +0.5 18,132 - -0.2 18,219 + +3.3 18,322 + ssb OKFHMN_00060 0.25 +0.3 18,322 + ssb OKFHMN_00060 0.25 +0.1 18,323 - ssb OKFHMN_00060 0.25 -2.8 18,381 + ssb OKFHMN_00060 0.38 -0.5 18,425 + ssb OKFHMN_00060 0.48 -0.5 18,449 + ssb OKFHMN_00060 0.53 +0.2 18,449 + ssb OKFHMN_00060 0.53 -0.3 18,594 - ssb OKFHMN_00060 0.85 +0.2 18,642 + +0.1 18,642 + -0.3 18,643 - -2.0 18,643 - +2.5 19,253 - +1.1 19,282 + OKFHMN_00065 0.12 +0.4 19,283 - OKFHMN_00065 0.12 -0.3 19,288 + OKFHMN_00065 0.14 -1.6 19,289 - OKFHMN_00065 0.15 +1.3 19,295 - OKFHMN_00065 0.17 -1.0 19,300 + OKFHMN_00065 0.19 +0.2 19,300 + OKFHMN_00065 0.19 -0.1 19,301 - OKFHMN_00065 0.20 -0.2 19,324 + OKFHMN_00065 0.30 -2.0 19,325 - OKFHMN_00065 0.30 -0.9 19,378 + OKFHMN_00065 0.53 -0.2 19,383 + OKFHMN_00065 0.55 +0.2 19,383 + OKFHMN_00065 0.55 -3.4 19,384 - OKFHMN_00065 0.56 -0.3 19,384 - OKFHMN_00065 0.56 -0.4 19,503 - +1.3 19,525 + +0.0 19,525 + +0.6
Or see this region's nucleotide sequence