Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mhpF and mhpD overlap by 4 nucleotides mhpD and mhpC are separated by 9 nucleotides mhpC and mhpB are separated by 17 nucleotides mhpB and mhpA are separated by 1 nucleotides
BNILDI_23090: mhpF - acetaldehyde dehydrogenase, at 4,648,027 to 4,648,977
mhpF
BNILDI_23095: mhpD - 2-keto-4-pentenoate hydratase, at 4,648,974 to 4,649,783
mhpD
BNILDI_23100: mhpC - 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase, at 4,649,793 to 4,650,659
mhpC
BNILDI_23105: mhpB - 3-carboxyethylcatechol 2,3-dioxygenase, at 4,650,677 to 4,651,621
mhpB
BNILDI_23110: mhpA - bifunctional 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase, at 4,651,623 to 4,653,287
mhpA
Position (kb) 4649
4650
4651 Strain fitness (log2 ratio) -2
-1
0
1 at 4648.830 kb on + strand, within mhpF at 4648.830 kb on + strand, within mhpF at 4648.966 kb on - strand at 4648.971 kb on + strand at 4649.028 kb on + strand at 4649.028 kb on + strand at 4649.028 kb on + strand at 4649.029 kb on - strand at 4649.029 kb on - strand at 4649.029 kb on - strand at 4649.085 kb on + strand, within mhpD at 4649.085 kb on + strand, within mhpD at 4649.085 kb on + strand, within mhpD at 4649.086 kb on - strand, within mhpD at 4649.106 kb on - strand, within mhpD at 4649.168 kb on + strand, within mhpD at 4649.293 kb on + strand, within mhpD at 4649.294 kb on - strand, within mhpD at 4649.294 kb on - strand, within mhpD at 4649.294 kb on - strand, within mhpD at 4649.294 kb on - strand, within mhpD at 4649.313 kb on - strand, within mhpD at 4649.404 kb on + strand, within mhpD at 4649.404 kb on + strand, within mhpD at 4649.404 kb on + strand, within mhpD at 4649.405 kb on - strand, within mhpD at 4649.405 kb on - strand, within mhpD at 4649.538 kb on + strand, within mhpD at 4649.538 kb on + strand, within mhpD at 4649.539 kb on - strand, within mhpD at 4649.641 kb on + strand, within mhpD at 4649.641 kb on + strand, within mhpD at 4649.653 kb on - strand, within mhpD at 4649.653 kb on - strand, within mhpD at 4649.668 kb on + strand, within mhpD at 4649.771 kb on + strand at 4649.771 kb on + strand at 4649.880 kb on - strand, within mhpC at 4649.880 kb on - strand, within mhpC at 4650.074 kb on + strand, within mhpC at 4650.074 kb on + strand, within mhpC at 4650.077 kb on + strand, within mhpC at 4650.078 kb on - strand, within mhpC at 4650.135 kb on - strand, within mhpC at 4650.162 kb on - strand, within mhpC at 4650.194 kb on + strand, within mhpC at 4650.194 kb on + strand, within mhpC at 4650.305 kb on + strand, within mhpC at 4650.575 kb on + strand at 4650.575 kb on + strand at 4650.575 kb on + strand at 4650.609 kb on + strand at 4650.653 kb on - strand at 4650.675 kb on - strand at 4650.675 kb on - strand at 4650.742 kb on - strand at 4650.872 kb on + strand, within mhpB at 4651.292 kb on - strand, within mhpB at 4651.292 kb on - strand, within mhpB at 4651.292 kb on - strand, within mhpB at 4651.296 kb on + strand, within mhpB at 4651.297 kb on - strand, within mhpB at 4651.447 kb on - strand, within mhpB at 4651.461 kb on + strand, within mhpB at 4651.611 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,648,830 + mhpF BNILDI_23090 0.84 -0.0 4,648,830 + mhpF BNILDI_23090 0.84 +1.3 4,648,966 - +0.3 4,648,971 + +0.4 4,649,028 + +0.3 4,649,028 + +0.2 4,649,028 + -0.5 4,649,029 - -0.5 4,649,029 - +0.6 4,649,029 - -1.2 4,649,085 + mhpD BNILDI_23095 0.14 +0.2 4,649,085 + mhpD BNILDI_23095 0.14 -2.3 4,649,085 + mhpD BNILDI_23095 0.14 +0.4 4,649,086 - mhpD BNILDI_23095 0.14 +0.3 4,649,106 - mhpD BNILDI_23095 0.16 +0.2 4,649,168 + mhpD BNILDI_23095 0.24 +0.1 4,649,293 + mhpD BNILDI_23095 0.39 -1.2 4,649,294 - mhpD BNILDI_23095 0.40 +0.6 4,649,294 - mhpD BNILDI_23095 0.40 -2.2 4,649,294 - mhpD BNILDI_23095 0.40 -0.3 4,649,294 - mhpD BNILDI_23095 0.40 +0.3 4,649,313 - mhpD BNILDI_23095 0.42 +1.0 4,649,404 + mhpD BNILDI_23095 0.53 -0.4 4,649,404 + mhpD BNILDI_23095 0.53 -0.1 4,649,404 + mhpD BNILDI_23095 0.53 -0.8 4,649,405 - mhpD BNILDI_23095 0.53 -0.1 4,649,405 - mhpD BNILDI_23095 0.53 +1.1 4,649,538 + mhpD BNILDI_23095 0.70 -0.2 4,649,538 + mhpD BNILDI_23095 0.70 +0.7 4,649,539 - mhpD BNILDI_23095 0.70 +0.3 4,649,641 + mhpD BNILDI_23095 0.82 -0.3 4,649,641 + mhpD BNILDI_23095 0.82 -0.9 4,649,653 - mhpD BNILDI_23095 0.84 +0.2 4,649,653 - mhpD BNILDI_23095 0.84 -0.2 4,649,668 + mhpD BNILDI_23095 0.86 +0.3 4,649,771 + -0.1 4,649,771 + +0.6 4,649,880 - mhpC BNILDI_23100 0.10 +0.5 4,649,880 - mhpC BNILDI_23100 0.10 -0.2 4,650,074 + mhpC BNILDI_23100 0.32 -1.3 4,650,074 + mhpC BNILDI_23100 0.32 -0.5 4,650,077 + mhpC BNILDI_23100 0.33 +0.6 4,650,078 - mhpC BNILDI_23100 0.33 -2.0 4,650,135 - mhpC BNILDI_23100 0.39 -0.3 4,650,162 - mhpC BNILDI_23100 0.43 +0.2 4,650,194 + mhpC BNILDI_23100 0.46 +0.7 4,650,194 + mhpC BNILDI_23100 0.46 -0.4 4,650,305 + mhpC BNILDI_23100 0.59 +1.2 4,650,575 + +0.0 4,650,575 + +1.3 4,650,575 + -0.1 4,650,609 + +0.2 4,650,653 - +1.6 4,650,675 - +0.9 4,650,675 - -0.9 4,650,742 - +0.8 4,650,872 + mhpB BNILDI_23105 0.21 -0.7 4,651,292 - mhpB BNILDI_23105 0.65 +1.0 4,651,292 - mhpB BNILDI_23105 0.65 -0.3 4,651,292 - mhpB BNILDI_23105 0.65 +1.3 4,651,296 + mhpB BNILDI_23105 0.66 +0.7 4,651,297 - mhpB BNILDI_23105 0.66 +0.4 4,651,447 - mhpB BNILDI_23105 0.81 -0.7 4,651,461 + mhpB BNILDI_23105 0.83 +0.6 4,651,611 + +0.1
Or see this region's nucleotide sequence