Strain Fitness in Escherichia coli ECRC62 around BNILDI_22950

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaroL and yaiI are separated by 182 nucleotidesyaiI and proC are separated by 119 nucleotidesproC and adrA are separated by 16 nucleotides BNILDI_22940: aroL - shikimate kinase AroL, at 4,617,999 to 4,618,523 aroL BNILDI_22945: yaiI - YaiI/YqxD family protein, at 4,618,706 to 4,619,164 yaiI BNILDI_22950: proC - pyrroline-5-carboxylate reductase, at 4,619,284 to 4,620,093 proC BNILDI_22955: adrA - diguanylate cyclase AdrA, at 4,620,110 to 4,621,225 adrA Position (kb) 4619 4620 4621Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2at 4618.320 kb on + strand, within aroLat 4618.398 kb on - strand, within aroLat 4618.541 kb on - strandat 4618.661 kb on - strandat 4618.662 kb on + strandat 4618.662 kb on + strandat 4618.663 kb on - strandat 4618.820 kb on + strand, within yaiIat 4618.821 kb on - strand, within yaiIat 4618.821 kb on - strand, within yaiIat 4618.821 kb on - strand, within yaiIat 4618.937 kb on + strand, within yaiIat 4618.938 kb on - strand, within yaiIat 4618.938 kb on - strand, within yaiIat 4618.938 kb on - strand, within yaiIat 4619.069 kb on + strand, within yaiIat 4619.092 kb on + strand, within yaiIat 4619.165 kb on - strandat 4619.220 kb on + strandat 4619.248 kb on + strandat 4619.386 kb on + strand, within proCat 4619.386 kb on + strand, within proCat 4619.387 kb on - strand, within proCat 4619.514 kb on + strand, within proCat 4619.730 kb on + strand, within proCat 4619.819 kb on + strand, within proCat 4619.819 kb on + strand, within proCat 4619.819 kb on + strand, within proCat 4619.820 kb on - strand, within proCat 4619.823 kb on + strand, within proCat 4619.823 kb on + strand, within proCat 4619.823 kb on + strand, within proCat 4619.823 kb on + strand, within proCat 4619.823 kb on + strand, within proCat 4619.824 kb on - strand, within proCat 4619.824 kb on - strand, within proCat 4619.824 kb on - strand, within proCat 4619.884 kb on + strand, within proCat 4619.965 kb on - strand, within proCat 4620.192 kb on + strandat 4620.232 kb on + strand, within adrAat 4620.233 kb on - strand, within adrAat 4620.296 kb on + strand, within adrAat 4620.341 kb on + strand, within adrAat 4620.402 kb on - strand, within adrAat 4620.527 kb on - strand, within adrAat 4620.547 kb on + strand, within adrAat 4620.547 kb on + strand, within adrAat 4620.547 kb on + strand, within adrAat 4620.568 kb on + strand, within adrAat 4620.570 kb on + strand, within adrAat 4620.648 kb on + strand, within adrAat 4620.839 kb on - strand, within adrA

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,618,320 + aroL BNILDI_22940 0.61 -0.8
4,618,398 - aroL BNILDI_22940 0.76 -0.7
4,618,541 - -0.1
4,618,661 - -0.2
4,618,662 + -0.9
4,618,662 + -0.0
4,618,663 - -1.4
4,618,820 + yaiI BNILDI_22945 0.25 +1.3
4,618,821 - yaiI BNILDI_22945 0.25 -1.2
4,618,821 - yaiI BNILDI_22945 0.25 -0.7
4,618,821 - yaiI BNILDI_22945 0.25 +2.3
4,618,937 + yaiI BNILDI_22945 0.50 +0.2
4,618,938 - yaiI BNILDI_22945 0.51 -0.4
4,618,938 - yaiI BNILDI_22945 0.51 +1.3
4,618,938 - yaiI BNILDI_22945 0.51 -0.4
4,619,069 + yaiI BNILDI_22945 0.79 +0.1
4,619,092 + yaiI BNILDI_22945 0.84 +0.1
4,619,165 - +0.1
4,619,220 + +0.2
4,619,248 + +2.0
4,619,386 + proC BNILDI_22950 0.13 -5.9
4,619,386 + proC BNILDI_22950 0.13 -2.8
4,619,387 - proC BNILDI_22950 0.13 -4.5
4,619,514 + proC BNILDI_22950 0.28 -2.6
4,619,730 + proC BNILDI_22950 0.55 -4.0
4,619,819 + proC BNILDI_22950 0.66 -4.9
4,619,819 + proC BNILDI_22950 0.66 -1.9
4,619,819 + proC BNILDI_22950 0.66 -5.4
4,619,820 - proC BNILDI_22950 0.66 -3.5
4,619,823 + proC BNILDI_22950 0.67 -6.2
4,619,823 + proC BNILDI_22950 0.67 -2.0
4,619,823 + proC BNILDI_22950 0.67 -2.8
4,619,823 + proC BNILDI_22950 0.67 -4.5
4,619,823 + proC BNILDI_22950 0.67 -1.1
4,619,824 - proC BNILDI_22950 0.67 -2.6
4,619,824 - proC BNILDI_22950 0.67 -1.2
4,619,824 - proC BNILDI_22950 0.67 -4.2
4,619,884 + proC BNILDI_22950 0.74 -1.0
4,619,965 - proC BNILDI_22950 0.84 -3.1
4,620,192 + +0.6
4,620,232 + adrA BNILDI_22955 0.11 -0.1
4,620,233 - adrA BNILDI_22955 0.11 +0.4
4,620,296 + adrA BNILDI_22955 0.17 +0.1
4,620,341 + adrA BNILDI_22955 0.21 -0.1
4,620,402 - adrA BNILDI_22955 0.26 -0.3
4,620,527 - adrA BNILDI_22955 0.37 +1.8
4,620,547 + adrA BNILDI_22955 0.39 -0.0
4,620,547 + adrA BNILDI_22955 0.39 -0.4
4,620,547 + adrA BNILDI_22955 0.39 +0.4
4,620,568 + adrA BNILDI_22955 0.41 -0.3
4,620,570 + adrA BNILDI_22955 0.41 -1.8
4,620,648 + adrA BNILDI_22955 0.48 +0.1
4,620,839 - adrA BNILDI_22955 0.65 -0.4

Or see this region's nucleotide sequence