Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lpxH and purE are separated by 117 nucleotides purE and purK overlap by 4 nucleotides purK and arcC are separated by 194 nucleotides
BNILDI_22250: lpxH - UDP-2,3-diacylglucosamine diphosphatase, at 4,470,360 to 4,471,082
lpxH
BNILDI_22255: purE - N5-carboxyaminoimidazole ribonucleotide mutase, at 4,471,200 to 4,471,709
purE
BNILDI_22260: purK - 5-(carboxyamino)imidazole ribonucleotide synthase, at 4,471,706 to 4,472,773
purK
BNILDI_22265: arcC - carbamate kinase, at 4,472,968 to 4,473,861
arcC
Position (kb) 4471
4472
4473 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4471.145 kb on + strand at 4471.398 kb on - strand, within purE at 4471.512 kb on + strand, within purE at 4471.523 kb on - strand, within purE at 4471.630 kb on + strand, within purE at 4471.631 kb on - strand, within purE at 4471.934 kb on - strand, within purK at 4472.002 kb on - strand, within purK at 4472.002 kb on - strand, within purK at 4472.002 kb on - strand, within purK at 4472.043 kb on + strand, within purK at 4472.063 kb on - strand, within purK at 4472.155 kb on + strand, within purK at 4472.156 kb on - strand, within purK at 4472.156 kb on - strand, within purK at 4472.248 kb on + strand, within purK at 4472.345 kb on - strand, within purK at 4472.360 kb on + strand, within purK at 4472.361 kb on - strand, within purK at 4472.437 kb on + strand, within purK at 4472.437 kb on + strand, within purK at 4472.579 kb on + strand, within purK at 4472.580 kb on - strand, within purK at 4472.644 kb on + strand, within purK at 4472.733 kb on - strand at 4472.762 kb on - strand at 4472.762 kb on - strand at 4472.762 kb on - strand at 4472.783 kb on + strand at 4472.804 kb on - strand at 4472.912 kb on - strand at 4472.921 kb on - strand at 4472.970 kb on - strand at 4473.603 kb on + strand, within arcC at 4473.604 kb on - strand, within arcC at 4473.604 kb on - strand, within arcC at 4473.604 kb on - strand, within arcC at 4473.604 kb on - strand, within arcC at 4473.604 kb on - strand, within arcC at 4473.606 kb on - strand, within arcC at 4473.659 kb on - strand, within arcC
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,471,145 + -0.1 4,471,398 - purE BNILDI_22255 0.39 -1.0 4,471,512 + purE BNILDI_22255 0.61 -3.7 4,471,523 - purE BNILDI_22255 0.63 -1.0 4,471,630 + purE BNILDI_22255 0.84 -4.2 4,471,631 - purE BNILDI_22255 0.85 -3.9 4,471,934 - purK BNILDI_22260 0.21 -3.5 4,472,002 - purK BNILDI_22260 0.28 -2.7 4,472,002 - purK BNILDI_22260 0.28 +1.0 4,472,002 - purK BNILDI_22260 0.28 -2.3 4,472,043 + purK BNILDI_22260 0.32 -3.1 4,472,063 - purK BNILDI_22260 0.33 -2.5 4,472,155 + purK BNILDI_22260 0.42 -3.2 4,472,156 - purK BNILDI_22260 0.42 -1.9 4,472,156 - purK BNILDI_22260 0.42 -3.0 4,472,248 + purK BNILDI_22260 0.51 -3.2 4,472,345 - purK BNILDI_22260 0.60 -2.3 4,472,360 + purK BNILDI_22260 0.61 -2.5 4,472,361 - purK BNILDI_22260 0.61 -3.0 4,472,437 + purK BNILDI_22260 0.68 -1.9 4,472,437 + purK BNILDI_22260 0.68 -2.2 4,472,579 + purK BNILDI_22260 0.82 -2.4 4,472,580 - purK BNILDI_22260 0.82 -3.0 4,472,644 + purK BNILDI_22260 0.88 -1.2 4,472,733 - +0.2 4,472,762 - -0.2 4,472,762 - +0.4 4,472,762 - +0.0 4,472,783 + +1.0 4,472,804 - +0.7 4,472,912 - +2.0 4,472,921 - +0.7 4,472,970 - -1.3 4,473,603 + arcC BNILDI_22265 0.71 +1.6 4,473,604 - arcC BNILDI_22265 0.71 +0.9 4,473,604 - arcC BNILDI_22265 0.71 -1.7 4,473,604 - arcC BNILDI_22265 0.71 +1.1 4,473,604 - arcC BNILDI_22265 0.71 -0.8 4,473,604 - arcC BNILDI_22265 0.71 -0.4 4,473,606 - arcC BNILDI_22265 0.71 +0.3 4,473,659 - arcC BNILDI_22265 0.77 +0.4
Or see this region's nucleotide sequence