Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppiB and lpxH are separated by 2 nucleotides lpxH and purE are separated by 117 nucleotides purE and purK overlap by 4 nucleotides
BNILDI_22245: ppiB - peptidylprolyl isomerase B, at 4,469,863 to 4,470,357
ppiB
BNILDI_22250: lpxH - UDP-2,3-diacylglucosamine diphosphatase, at 4,470,360 to 4,471,082
lpxH
BNILDI_22255: purE - N5-carboxyaminoimidazole ribonucleotide mutase, at 4,471,200 to 4,471,709
purE
BNILDI_22260: purK - 5-(carboxyamino)imidazole ribonucleotide synthase, at 4,471,706 to 4,472,773
purK
Position (kb)
4471
4472 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4471.145 kb on + strand at 4471.398 kb on - strand, within purE at 4471.512 kb on + strand, within purE at 4471.523 kb on - strand, within purE at 4471.630 kb on + strand, within purE at 4471.631 kb on - strand, within purE at 4471.934 kb on - strand, within purK at 4472.002 kb on - strand, within purK at 4472.002 kb on - strand, within purK at 4472.002 kb on - strand, within purK at 4472.043 kb on + strand, within purK at 4472.063 kb on - strand, within purK at 4472.155 kb on + strand, within purK at 4472.156 kb on - strand, within purK at 4472.156 kb on - strand, within purK at 4472.248 kb on + strand, within purK at 4472.345 kb on - strand, within purK at 4472.360 kb on + strand, within purK at 4472.361 kb on - strand, within purK at 4472.437 kb on + strand, within purK at 4472.437 kb on + strand, within purK at 4472.579 kb on + strand, within purK at 4472.580 kb on - strand, within purK at 4472.644 kb on + strand, within purK
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,471,145 + -0.1 4,471,398 - purE BNILDI_22255 0.39 -1.0 4,471,512 + purE BNILDI_22255 0.61 -3.7 4,471,523 - purE BNILDI_22255 0.63 -1.0 4,471,630 + purE BNILDI_22255 0.84 -4.2 4,471,631 - purE BNILDI_22255 0.85 -3.9 4,471,934 - purK BNILDI_22260 0.21 -3.5 4,472,002 - purK BNILDI_22260 0.28 -2.7 4,472,002 - purK BNILDI_22260 0.28 +1.0 4,472,002 - purK BNILDI_22260 0.28 -2.3 4,472,043 + purK BNILDI_22260 0.32 -3.1 4,472,063 - purK BNILDI_22260 0.33 -2.5 4,472,155 + purK BNILDI_22260 0.42 -3.2 4,472,156 - purK BNILDI_22260 0.42 -1.9 4,472,156 - purK BNILDI_22260 0.42 -3.0 4,472,248 + purK BNILDI_22260 0.51 -3.2 4,472,345 - purK BNILDI_22260 0.60 -2.3 4,472,360 + purK BNILDI_22260 0.61 -2.5 4,472,361 - purK BNILDI_22260 0.61 -3.0 4,472,437 + purK BNILDI_22260 0.68 -1.9 4,472,437 + purK BNILDI_22260 0.68 -2.2 4,472,579 + purK BNILDI_22260 0.82 -2.4 4,472,580 - purK BNILDI_22260 0.82 -3.0 4,472,644 + purK BNILDI_22260 0.88 -1.2
Or see this region's nucleotide sequence