Strain Fitness in Escherichia coli ECRC62 around BNILDI_22255

Experiment: Sodium-DL-Lactate

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntppiB and lpxH are separated by 2 nucleotideslpxH and purE are separated by 117 nucleotidespurE and purK overlap by 4 nucleotides BNILDI_22245: ppiB - peptidylprolyl isomerase B, at 4,469,863 to 4,470,357 ppiB BNILDI_22250: lpxH - UDP-2,3-diacylglucosamine diphosphatase, at 4,470,360 to 4,471,082 lpxH BNILDI_22255: purE - N5-carboxyaminoimidazole ribonucleotide mutase, at 4,471,200 to 4,471,709 purE BNILDI_22260: purK - 5-(carboxyamino)imidazole ribonucleotide synthase, at 4,471,706 to 4,472,773 purK Position (kb) 4471 4472Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4471.145 kb on + strandat 4471.398 kb on - strand, within purEat 4471.512 kb on + strand, within purEat 4471.523 kb on - strand, within purEat 4471.630 kb on + strand, within purEat 4471.631 kb on - strand, within purEat 4471.934 kb on - strand, within purKat 4472.002 kb on - strand, within purKat 4472.002 kb on - strand, within purKat 4472.002 kb on - strand, within purKat 4472.043 kb on + strand, within purKat 4472.063 kb on - strand, within purKat 4472.155 kb on + strand, within purKat 4472.156 kb on - strand, within purKat 4472.156 kb on - strand, within purKat 4472.248 kb on + strand, within purKat 4472.345 kb on - strand, within purKat 4472.360 kb on + strand, within purKat 4472.361 kb on - strand, within purKat 4472.437 kb on + strand, within purKat 4472.437 kb on + strand, within purKat 4472.579 kb on + strand, within purKat 4472.580 kb on - strand, within purKat 4472.644 kb on + strand, within purK

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
remove
4,471,145 + -0.1
4,471,398 - purE BNILDI_22255 0.39 -1.0
4,471,512 + purE BNILDI_22255 0.61 -3.7
4,471,523 - purE BNILDI_22255 0.63 -1.0
4,471,630 + purE BNILDI_22255 0.84 -4.2
4,471,631 - purE BNILDI_22255 0.85 -3.9
4,471,934 - purK BNILDI_22260 0.21 -3.5
4,472,002 - purK BNILDI_22260 0.28 -2.7
4,472,002 - purK BNILDI_22260 0.28 +1.0
4,472,002 - purK BNILDI_22260 0.28 -2.3
4,472,043 + purK BNILDI_22260 0.32 -3.1
4,472,063 - purK BNILDI_22260 0.33 -2.5
4,472,155 + purK BNILDI_22260 0.42 -3.2
4,472,156 - purK BNILDI_22260 0.42 -1.9
4,472,156 - purK BNILDI_22260 0.42 -3.0
4,472,248 + purK BNILDI_22260 0.51 -3.2
4,472,345 - purK BNILDI_22260 0.60 -2.3
4,472,360 + purK BNILDI_22260 0.61 -2.5
4,472,361 - purK BNILDI_22260 0.61 -3.0
4,472,437 + purK BNILDI_22260 0.68 -1.9
4,472,437 + purK BNILDI_22260 0.68 -2.2
4,472,579 + purK BNILDI_22260 0.82 -2.4
4,472,580 - purK BNILDI_22260 0.82 -3.0
4,472,644 + purK BNILDI_22260 0.88 -1.2

Or see this region's nucleotide sequence