Strain Fitness in Escherichia coli ECRC62 around BNILDI_21775

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdacA and ybeD are separated by 110 nucleotidesybeD and lipB are separated by 100 nucleotideslipB and ybeF are separated by 258 nucleotides BNILDI_21765: dacA - D-alanyl-D-alanine carboxypeptidase DacA, at 4,375,939 to 4,377,150 dacA BNILDI_21770: ybeD - DUF493 domain-containing protein, at 4,377,261 to 4,377,524 ybeD BNILDI_21775: lipB - lipoyl(octanoyl) transferase LipB, at 4,377,625 to 4,378,266 lipB BNILDI_21780: ybeF - YbeF family transcriptional regulator, at 4,378,525 to 4,379,478 ybeF Position (kb) 4377 4378 4379Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 4376.754 kb on + strand, within dacAat 4376.760 kb on + strand, within dacAat 4376.761 kb on - strand, within dacAat 4376.761 kb on - strand, within dacAat 4376.959 kb on + strand, within dacAat 4376.960 kb on - strand, within dacAat 4376.960 kb on - strand, within dacAat 4377.171 kb on + strandat 4377.188 kb on + strandat 4377.191 kb on + strandat 4377.237 kb on + strandat 4377.237 kb on + strandat 4377.582 kb on + strandat 4377.582 kb on + strandat 4377.583 kb on - strandat 4377.599 kb on + strandat 4377.670 kb on + strandat 4377.670 kb on + strandat 4377.892 kb on - strand, within lipBat 4378.310 kb on - strandat 4378.467 kb on + strandat 4378.468 kb on - strandat 4378.644 kb on - strand, within ybeFat 4378.646 kb on - strand, within ybeFat 4378.734 kb on - strand, within ybeFat 4378.807 kb on + strand, within ybeFat 4378.807 kb on + strand, within ybeFat 4378.807 kb on + strand, within ybeFat 4378.808 kb on - strand, within ybeFat 4378.808 kb on - strand, within ybeFat 4378.810 kb on - strand, within ybeFat 4378.861 kb on + strand, within ybeFat 4378.862 kb on - strand, within ybeFat 4378.885 kb on - strand, within ybeFat 4378.964 kb on + strand, within ybeFat 4379.096 kb on + strand, within ybeFat 4379.129 kb on + strand, within ybeFat 4379.130 kb on - strand, within ybeFat 4379.169 kb on - strand, within ybeFat 4379.257 kb on + strand, within ybeF

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,376,754 + dacA BNILDI_21765 0.67 +0.5
4,376,760 + dacA BNILDI_21765 0.68 +1.2
4,376,761 - dacA BNILDI_21765 0.68 -2.4
4,376,761 - dacA BNILDI_21765 0.68 +0.1
4,376,959 + dacA BNILDI_21765 0.84 +1.0
4,376,960 - dacA BNILDI_21765 0.84 -0.3
4,376,960 - dacA BNILDI_21765 0.84 +0.0
4,377,171 + +1.8
4,377,188 + -0.8
4,377,191 + +1.0
4,377,237 + +0.3
4,377,237 + -2.0
4,377,582 + -1.2
4,377,582 + -1.4
4,377,583 - -0.1
4,377,599 + -0.2
4,377,670 + -1.6
4,377,670 + -2.7
4,377,892 - lipB BNILDI_21775 0.42 -3.9
4,378,310 - -0.8
4,378,467 + +0.6
4,378,468 - +1.0
4,378,644 - ybeF BNILDI_21780 0.12 -0.6
4,378,646 - ybeF BNILDI_21780 0.13 +0.0
4,378,734 - ybeF BNILDI_21780 0.22 +0.4
4,378,807 + ybeF BNILDI_21780 0.30 -0.3
4,378,807 + ybeF BNILDI_21780 0.30 +0.8
4,378,807 + ybeF BNILDI_21780 0.30 -0.5
4,378,808 - ybeF BNILDI_21780 0.30 -2.1
4,378,808 - ybeF BNILDI_21780 0.30 +0.2
4,378,810 - ybeF BNILDI_21780 0.30 -0.0
4,378,861 + ybeF BNILDI_21780 0.35 +0.6
4,378,862 - ybeF BNILDI_21780 0.35 +0.8
4,378,885 - ybeF BNILDI_21780 0.38 -1.0
4,378,964 + ybeF BNILDI_21780 0.46 -1.6
4,379,096 + ybeF BNILDI_21780 0.60 +0.4
4,379,129 + ybeF BNILDI_21780 0.63 -0.4
4,379,130 - ybeF BNILDI_21780 0.63 +0.2
4,379,169 - ybeF BNILDI_21780 0.68 -0.5
4,379,257 + ybeF BNILDI_21780 0.77 +1.5

Or see this region's nucleotide sequence