Strain Fitness in Escherichia coli ECRC62 around BNILDI_21375

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntabrB and nei overlap by 4 nucleotidesnei and pxpA are separated by 35 nucleotidespxpA and pxpC overlap by 11 nucleotidespxpC and pxpB overlap by 7 nucleotides BNILDI_21365: abrB - Putative regulator AbrB, at 4,297,908 to 4,298,954 abrB BNILDI_21370: nei - endonuclease VIII, at 4,298,951 to 4,299,742 nei BNILDI_21375: pxpA - 5-oxoprolinase subunit PxpA, at 4,299,778 to 4,300,512 pxpA BNILDI_21380: pxpC - 5-oxoprolinase subunit PxpC, at 4,300,502 to 4,301,434 pxpC BNILDI_21385: pxpB - 5-oxoprolinase subunit PxpB, at 4,301,428 to 4,302,084 pxpB Position (kb) 4299 4300 4301Strain fitness (log2 ratio) -1 0 1at 4298.781 kb on + strand, within abrBat 4298.782 kb on - strand, within abrBat 4298.855 kb on + strandat 4298.858 kb on + strandat 4298.859 kb on - strandat 4298.922 kb on - strandat 4298.932 kb on - strandat 4298.932 kb on - strandat 4298.952 kb on + strandat 4298.977 kb on - strandat 4299.059 kb on - strand, within neiat 4299.085 kb on + strand, within neiat 4299.187 kb on + strand, within neiat 4299.431 kb on - strand, within neiat 4299.468 kb on - strand, within neiat 4299.554 kb on + strand, within neiat 4299.555 kb on - strand, within neiat 4299.587 kb on - strand, within neiat 4299.674 kb on + strandat 4299.767 kb on - strandat 4299.780 kb on - strandat 4299.814 kb on - strandat 4299.906 kb on + strand, within pxpAat 4299.907 kb on - strand, within pxpAat 4299.907 kb on - strand, within pxpAat 4299.938 kb on - strand, within pxpAat 4300.131 kb on - strand, within pxpAat 4300.431 kb on + strand, within pxpAat 4300.456 kb on + strandat 4300.456 kb on + strandat 4300.456 kb on + strandat 4300.457 kb on - strandat 4300.636 kb on - strand, within pxpCat 4300.636 kb on - strand, within pxpCat 4300.639 kb on + strand, within pxpCat 4300.639 kb on + strand, within pxpCat 4300.640 kb on - strand, within pxpCat 4300.640 kb on - strand, within pxpCat 4300.640 kb on - strand, within pxpCat 4300.721 kb on - strand, within pxpCat 4300.802 kb on + strand, within pxpCat 4300.834 kb on + strand, within pxpCat 4300.835 kb on - strand, within pxpCat 4300.835 kb on - strand, within pxpCat 4300.891 kb on - strand, within pxpCat 4301.080 kb on + strand, within pxpCat 4301.405 kb on - strandat 4301.405 kb on - strandat 4301.405 kb on - strandat 4301.406 kb on + strandat 4301.406 kb on + strandat 4301.406 kb on + strandat 4301.406 kb on + strandat 4301.406 kb on + strandat 4301.407 kb on - strandat 4301.407 kb on - strandat 4301.480 kb on - strandat 4301.483 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,298,781 + abrB BNILDI_21365 0.83 +1.0
4,298,782 - abrB BNILDI_21365 0.83 -0.8
4,298,855 + +1.0
4,298,858 + -0.2
4,298,859 - -0.8
4,298,922 - +1.1
4,298,932 - +0.4
4,298,932 - +1.2
4,298,952 + -0.3
4,298,977 - +0.2
4,299,059 - nei BNILDI_21370 0.14 -0.8
4,299,085 + nei BNILDI_21370 0.17 -1.0
4,299,187 + nei BNILDI_21370 0.30 +0.2
4,299,431 - nei BNILDI_21370 0.61 +0.5
4,299,468 - nei BNILDI_21370 0.65 -1.1
4,299,554 + nei BNILDI_21370 0.76 -0.4
4,299,555 - nei BNILDI_21370 0.76 +0.7
4,299,587 - nei BNILDI_21370 0.80 +0.1
4,299,674 + -0.1
4,299,767 - -1.4
4,299,780 - +1.4
4,299,814 - +1.1
4,299,906 + pxpA BNILDI_21375 0.17 +0.4
4,299,907 - pxpA BNILDI_21375 0.18 +0.4
4,299,907 - pxpA BNILDI_21375 0.18 +0.6
4,299,938 - pxpA BNILDI_21375 0.22 +1.2
4,300,131 - pxpA BNILDI_21375 0.48 -1.4
4,300,431 + pxpA BNILDI_21375 0.89 +0.1
4,300,456 + +1.2
4,300,456 + +0.1
4,300,456 + +0.1
4,300,457 - +1.3
4,300,636 - pxpC BNILDI_21380 0.14 +0.3
4,300,636 - pxpC BNILDI_21380 0.14 +0.6
4,300,639 + pxpC BNILDI_21380 0.15 +0.6
4,300,639 + pxpC BNILDI_21380 0.15 -0.5
4,300,640 - pxpC BNILDI_21380 0.15 -0.4
4,300,640 - pxpC BNILDI_21380 0.15 -0.0
4,300,640 - pxpC BNILDI_21380 0.15 +1.1
4,300,721 - pxpC BNILDI_21380 0.23 -0.3
4,300,802 + pxpC BNILDI_21380 0.32 -0.8
4,300,834 + pxpC BNILDI_21380 0.36 +0.2
4,300,835 - pxpC BNILDI_21380 0.36 +0.1
4,300,835 - pxpC BNILDI_21380 0.36 +0.8
4,300,891 - pxpC BNILDI_21380 0.42 -0.5
4,301,080 + pxpC BNILDI_21380 0.62 -0.3
4,301,405 - +0.1
4,301,405 - +0.3
4,301,405 - -0.6
4,301,406 + +0.2
4,301,406 + +0.2
4,301,406 + +0.5
4,301,406 + +0.0
4,301,406 + +0.2
4,301,407 - +0.3
4,301,407 - -0.0
4,301,480 - -0.7
4,301,483 - -0.7

Or see this region's nucleotide sequence