Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolA and tolR are separated by 64 nucleotides tolR and tolQ are separated by 3 nucleotides tolQ and ybgC overlap by 4 nucleotides ybgC and ybgE are separated by 149 nucleotides ybgE and cydX overlap by 1 nucleotides cydX and cydB are separated by 14 nucleotides
BNILDI_21240: tolA - cell envelope integrity protein TolA, at 4,268,311 to 4,269,609
tolA
BNILDI_21245: tolR - colicin uptake protein TolR, at 4,269,674 to 4,270,102
tolR
BNILDI_21250: tolQ - Tol-Pal system protein TolQ, at 4,270,106 to 4,270,798
tolQ
BNILDI_21255: ybgC - tol-pal system-associated acyl-CoA thioesterase, at 4,270,795 to 4,271,199
ybgC
BNILDI_21260: ybgE - cyd operon protein YbgE, at 4,271,349 to 4,271,642
ybgE
BNILDI_21265: cydX - cytochrome bd-I oxidase subunit CydX, at 4,271,642 to 4,271,755
cydX
BNILDI_21270: cydB - cytochrome d ubiquinol oxidase subunit II, at 4,271,770 to 4,272,909
cydB
Position (kb)
4270
4271 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4269.150 kb on + strand, within tolA at 4269.419 kb on + strand, within tolA at 4269.419 kb on + strand, within tolA at 4269.497 kb on - strand at 4269.562 kb on - strand at 4269.675 kb on + strand at 4269.675 kb on + strand at 4269.693 kb on - strand at 4269.723 kb on - strand, within tolR at 4269.744 kb on - strand, within tolR at 4270.225 kb on - strand, within tolQ at 4270.384 kb on - strand, within tolQ at 4270.459 kb on + strand, within tolQ at 4270.466 kb on - strand, within tolQ at 4270.896 kb on + strand, within ybgC at 4270.897 kb on - strand, within ybgC at 4270.915 kb on - strand, within ybgC at 4270.915 kb on - strand, within ybgC at 4270.966 kb on + strand, within ybgC at 4271.004 kb on - strand, within ybgC at 4271.019 kb on + strand, within ybgC at 4271.019 kb on + strand, within ybgC at 4271.020 kb on - strand, within ybgC at 4271.024 kb on - strand, within ybgC at 4271.129 kb on + strand, within ybgC at 4271.130 kb on - strand, within ybgC at 4271.152 kb on - strand, within ybgC at 4271.194 kb on - strand at 4271.203 kb on - strand at 4271.203 kb on - strand at 4271.246 kb on + strand at 4271.250 kb on - strand at 4271.250 kb on - strand at 4271.251 kb on + strand at 4271.252 kb on - strand at 4271.325 kb on + strand at 4271.487 kb on + strand, within ybgE at 4271.549 kb on + strand, within ybgE at 4271.549 kb on + strand, within ybgE at 4271.550 kb on - strand, within ybgE
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,269,150 + tolA BNILDI_21240 0.65 -1.4 4,269,419 + tolA BNILDI_21240 0.85 -4.5 4,269,419 + tolA BNILDI_21240 0.85 -3.0 4,269,497 - -1.3 4,269,562 - -2.1 4,269,675 + +0.4 4,269,675 + +1.0 4,269,693 - +0.7 4,269,723 - tolR BNILDI_21245 0.11 -2.0 4,269,744 - tolR BNILDI_21245 0.16 -1.2 4,270,225 - tolQ BNILDI_21250 0.17 -1.0 4,270,384 - tolQ BNILDI_21250 0.40 -3.4 4,270,459 + tolQ BNILDI_21250 0.51 -2.3 4,270,466 - tolQ BNILDI_21250 0.52 -2.4 4,270,896 + ybgC BNILDI_21255 0.25 -1.3 4,270,897 - ybgC BNILDI_21255 0.25 -0.2 4,270,915 - ybgC BNILDI_21255 0.30 +0.2 4,270,915 - ybgC BNILDI_21255 0.30 +0.0 4,270,966 + ybgC BNILDI_21255 0.42 -3.3 4,271,004 - ybgC BNILDI_21255 0.52 +0.4 4,271,019 + ybgC BNILDI_21255 0.55 -0.5 4,271,019 + ybgC BNILDI_21255 0.55 -2.8 4,271,020 - ybgC BNILDI_21255 0.56 +0.2 4,271,024 - ybgC BNILDI_21255 0.57 -1.1 4,271,129 + ybgC BNILDI_21255 0.82 -1.6 4,271,130 - ybgC BNILDI_21255 0.83 -0.7 4,271,152 - ybgC BNILDI_21255 0.88 -0.1 4,271,194 - +0.5 4,271,203 - +0.2 4,271,203 - -0.7 4,271,246 + -0.8 4,271,250 - -0.1 4,271,250 - -0.3 4,271,251 + +0.4 4,271,252 - +0.0 4,271,325 + +0.7 4,271,487 + ybgE BNILDI_21260 0.47 +0.6 4,271,549 + ybgE BNILDI_21260 0.68 -0.3 4,271,549 + ybgE BNILDI_21260 0.68 +0.1 4,271,550 - ybgE BNILDI_21260 0.68 -0.8
Or see this region's nucleotide sequence