Strain Fitness in Escherichia coli ECRC62 around BNILDI_21250

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttolA and tolR are separated by 64 nucleotidestolR and tolQ are separated by 3 nucleotidestolQ and ybgC overlap by 4 nucleotidesybgC and ybgE are separated by 149 nucleotidesybgE and cydX overlap by 1 nucleotidescydX and cydB are separated by 14 nucleotides BNILDI_21240: tolA - cell envelope integrity protein TolA, at 4,268,311 to 4,269,609 tolA BNILDI_21245: tolR - colicin uptake protein TolR, at 4,269,674 to 4,270,102 tolR BNILDI_21250: tolQ - Tol-Pal system protein TolQ, at 4,270,106 to 4,270,798 tolQ BNILDI_21255: ybgC - tol-pal system-associated acyl-CoA thioesterase, at 4,270,795 to 4,271,199 ybgC BNILDI_21260: ybgE - cyd operon protein YbgE, at 4,271,349 to 4,271,642 ybgE BNILDI_21265: cydX - cytochrome bd-I oxidase subunit CydX, at 4,271,642 to 4,271,755 cydX BNILDI_21270: cydB - cytochrome d ubiquinol oxidase subunit II, at 4,271,770 to 4,272,909 cydB Position (kb) 4270 4271Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4269.150 kb on + strand, within tolAat 4269.419 kb on + strand, within tolAat 4269.419 kb on + strand, within tolAat 4269.497 kb on - strandat 4269.562 kb on - strandat 4269.675 kb on + strandat 4269.675 kb on + strandat 4269.693 kb on - strandat 4269.723 kb on - strand, within tolRat 4269.744 kb on - strand, within tolRat 4270.225 kb on - strand, within tolQat 4270.384 kb on - strand, within tolQat 4270.459 kb on + strand, within tolQat 4270.466 kb on - strand, within tolQat 4270.896 kb on + strand, within ybgCat 4270.897 kb on - strand, within ybgCat 4270.915 kb on - strand, within ybgCat 4270.915 kb on - strand, within ybgCat 4270.966 kb on + strand, within ybgCat 4271.004 kb on - strand, within ybgCat 4271.019 kb on + strand, within ybgCat 4271.019 kb on + strand, within ybgCat 4271.020 kb on - strand, within ybgCat 4271.024 kb on - strand, within ybgCat 4271.129 kb on + strand, within ybgCat 4271.130 kb on - strand, within ybgCat 4271.152 kb on - strand, within ybgCat 4271.194 kb on - strandat 4271.203 kb on - strandat 4271.203 kb on - strandat 4271.246 kb on + strandat 4271.250 kb on - strandat 4271.250 kb on - strandat 4271.251 kb on + strandat 4271.252 kb on - strandat 4271.325 kb on + strandat 4271.487 kb on + strand, within ybgEat 4271.549 kb on + strand, within ybgEat 4271.549 kb on + strand, within ybgEat 4271.550 kb on - strand, within ybgE

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,269,150 + tolA BNILDI_21240 0.65 -1.4
4,269,419 + tolA BNILDI_21240 0.85 -4.5
4,269,419 + tolA BNILDI_21240 0.85 -3.0
4,269,497 - -1.3
4,269,562 - -2.1
4,269,675 + +0.4
4,269,675 + +1.0
4,269,693 - +0.7
4,269,723 - tolR BNILDI_21245 0.11 -2.0
4,269,744 - tolR BNILDI_21245 0.16 -1.2
4,270,225 - tolQ BNILDI_21250 0.17 -1.0
4,270,384 - tolQ BNILDI_21250 0.40 -3.4
4,270,459 + tolQ BNILDI_21250 0.51 -2.3
4,270,466 - tolQ BNILDI_21250 0.52 -2.4
4,270,896 + ybgC BNILDI_21255 0.25 -1.3
4,270,897 - ybgC BNILDI_21255 0.25 -0.2
4,270,915 - ybgC BNILDI_21255 0.30 +0.2
4,270,915 - ybgC BNILDI_21255 0.30 +0.0
4,270,966 + ybgC BNILDI_21255 0.42 -3.3
4,271,004 - ybgC BNILDI_21255 0.52 +0.4
4,271,019 + ybgC BNILDI_21255 0.55 -0.5
4,271,019 + ybgC BNILDI_21255 0.55 -2.8
4,271,020 - ybgC BNILDI_21255 0.56 +0.2
4,271,024 - ybgC BNILDI_21255 0.57 -1.1
4,271,129 + ybgC BNILDI_21255 0.82 -1.6
4,271,130 - ybgC BNILDI_21255 0.83 -0.7
4,271,152 - ybgC BNILDI_21255 0.88 -0.1
4,271,194 - +0.5
4,271,203 - +0.2
4,271,203 - -0.7
4,271,246 + -0.8
4,271,250 - -0.1
4,271,250 - -0.3
4,271,251 + +0.4
4,271,252 - +0.0
4,271,325 + +0.7
4,271,487 + ybgE BNILDI_21260 0.47 +0.6
4,271,549 + ybgE BNILDI_21260 0.68 -0.3
4,271,549 + ybgE BNILDI_21260 0.68 +0.1
4,271,550 - ybgE BNILDI_21260 0.68 -0.8

Or see this region's nucleotide sequence