Strain Fitness in Escherichia coli ECRC62 around BNILDI_20330

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpflB and pflA are separated by 191 nucleotidespflA and ycaK are separated by 81 nucleotidesycaK and ycaN are separated by 99 nucleotides BNILDI_20325: pflB - formate C-acetyltransferase, at 4,088,119 to 4,090,401 pflB BNILDI_20330: pflA - pyruvate formate lyase 1-activating protein, at 4,090,593 to 4,091,333 pflA BNILDI_20335: ycaK - NAD(P)H oxidoreductase, at 4,091,415 to 4,092,005 ycaK BNILDI_20340: ycaN - Uncharacterized HTH-type transcriptional regulator YcaN, at 4,092,105 to 4,093,013 ycaN Position (kb) 4090 4091 4092Strain fitness (log2 ratio) -1 0 1 2at 4089.617 kb on - strand, within pflBat 4089.632 kb on - strand, within pflBat 4089.840 kb on - strand, within pflBat 4089.946 kb on - strand, within pflBat 4089.946 kb on - strand, within pflBat 4089.956 kb on - strand, within pflBat 4089.956 kb on - strand, within pflBat 4089.956 kb on - strand, within pflBat 4089.960 kb on + strand, within pflBat 4090.093 kb on + strand, within pflBat 4090.107 kb on + strand, within pflBat 4090.114 kb on + strand, within pflBat 4090.196 kb on - strandat 4090.375 kb on - strandat 4090.380 kb on + strandat 4090.395 kb on + strandat 4090.396 kb on - strandat 4090.396 kb on - strandat 4090.399 kb on + strandat 4090.400 kb on - strandat 4090.404 kb on - strandat 4090.435 kb on + strandat 4090.494 kb on + strandat 4090.495 kb on - strandat 4090.498 kb on + strandat 4090.499 kb on - strandat 4090.500 kb on + strandat 4090.652 kb on + strandat 4090.653 kb on - strandat 4090.653 kb on - strandat 4090.780 kb on - strand, within pflAat 4091.163 kb on - strand, within pflAat 4091.242 kb on - strand, within pflAat 4091.267 kb on + strandat 4091.310 kb on + strandat 4091.324 kb on - strandat 4091.331 kb on + strandat 4091.416 kb on + strandat 4091.419 kb on - strandat 4091.427 kb on + strandat 4091.428 kb on - strandat 4091.428 kb on - strandat 4091.449 kb on - strandat 4091.652 kb on + strand, within ycaKat 4091.692 kb on - strand, within ycaKat 4091.702 kb on + strand, within ycaKat 4091.703 kb on - strand, within ycaKat 4091.703 kb on - strand, within ycaKat 4091.713 kb on - strand, within ycaKat 4091.901 kb on + strand, within ycaKat 4091.942 kb on + strand, within ycaKat 4091.942 kb on + strand, within ycaKat 4091.986 kb on + strandat 4091.990 kb on + strandat 4092.062 kb on + strandat 4092.216 kb on - strand, within ycaNat 4092.218 kb on + strand, within ycaNat 4092.219 kb on - strand, within ycaNat 4092.251 kb on - strand, within ycaNat 4092.297 kb on - strand, within ycaNat 4092.297 kb on - strand, within ycaN

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,089,617 - pflB BNILDI_20325 0.66 -0.0
4,089,632 - pflB BNILDI_20325 0.66 -0.9
4,089,840 - pflB BNILDI_20325 0.75 +0.0
4,089,946 - pflB BNILDI_20325 0.80 -1.1
4,089,946 - pflB BNILDI_20325 0.80 +0.6
4,089,956 - pflB BNILDI_20325 0.80 +0.1
4,089,956 - pflB BNILDI_20325 0.80 +0.1
4,089,956 - pflB BNILDI_20325 0.80 -0.7
4,089,960 + pflB BNILDI_20325 0.81 +0.7
4,090,093 + pflB BNILDI_20325 0.86 +0.5
4,090,107 + pflB BNILDI_20325 0.87 +0.2
4,090,114 + pflB BNILDI_20325 0.87 -0.2
4,090,196 - -0.1
4,090,375 - +0.3
4,090,380 + +0.7
4,090,395 + -0.0
4,090,396 - -1.2
4,090,396 - +1.5
4,090,399 + +1.2
4,090,400 - -0.9
4,090,404 - -0.4
4,090,435 + +0.3
4,090,494 + -0.7
4,090,495 - -0.0
4,090,498 + +0.3
4,090,499 - -0.4
4,090,500 + +0.1
4,090,652 + +0.4
4,090,653 - +1.0
4,090,653 - +0.2
4,090,780 - pflA BNILDI_20330 0.25 -1.5
4,091,163 - pflA BNILDI_20330 0.77 +0.9
4,091,242 - pflA BNILDI_20330 0.88 -0.7
4,091,267 + +2.1
4,091,310 + -1.4
4,091,324 - -0.7
4,091,331 + +0.4
4,091,416 + +1.0
4,091,419 - +0.4
4,091,427 + -0.0
4,091,428 - -0.2
4,091,428 - +0.5
4,091,449 - +0.6
4,091,652 + ycaK BNILDI_20335 0.40 -0.2
4,091,692 - ycaK BNILDI_20335 0.47 +1.6
4,091,702 + ycaK BNILDI_20335 0.49 +0.2
4,091,703 - ycaK BNILDI_20335 0.49 -0.4
4,091,703 - ycaK BNILDI_20335 0.49 -0.8
4,091,713 - ycaK BNILDI_20335 0.50 -0.9
4,091,901 + ycaK BNILDI_20335 0.82 -0.8
4,091,942 + ycaK BNILDI_20335 0.89 +0.4
4,091,942 + ycaK BNILDI_20335 0.89 -0.4
4,091,986 + +0.0
4,091,990 + -0.5
4,092,062 + -1.5
4,092,216 - ycaN BNILDI_20340 0.12 -0.0
4,092,218 + ycaN BNILDI_20340 0.12 +0.7
4,092,219 - ycaN BNILDI_20340 0.13 -1.0
4,092,251 - ycaN BNILDI_20340 0.16 -0.5
4,092,297 - ycaN BNILDI_20340 0.21 +0.2
4,092,297 - ycaN BNILDI_20340 0.21 -0.9

Or see this region's nucleotide sequence