Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pflB and pflA are separated by 191 nucleotides pflA and ycaK are separated by 81 nucleotides ycaK and ycaN are separated by 99 nucleotides
BNILDI_20325: pflB - formate C-acetyltransferase, at 4,088,119 to 4,090,401
pflB
BNILDI_20330: pflA - pyruvate formate lyase 1-activating protein, at 4,090,593 to 4,091,333
pflA
BNILDI_20335: ycaK - NAD(P)H oxidoreductase, at 4,091,415 to 4,092,005
ycaK
BNILDI_20340: ycaN - Uncharacterized HTH-type transcriptional regulator YcaN, at 4,092,105 to 4,093,013
ycaN
Position (kb)
4090
4091
4092 Strain fitness (log2 ratio)
-1
0
1
2 at 4089.617 kb on - strand, within pflB at 4089.632 kb on - strand, within pflB at 4089.840 kb on - strand, within pflB at 4089.946 kb on - strand, within pflB at 4089.946 kb on - strand, within pflB at 4089.956 kb on - strand, within pflB at 4089.956 kb on - strand, within pflB at 4089.956 kb on - strand, within pflB at 4089.960 kb on + strand, within pflB at 4090.093 kb on + strand, within pflB at 4090.107 kb on + strand, within pflB at 4090.114 kb on + strand, within pflB at 4090.196 kb on - strand at 4090.375 kb on - strand at 4090.380 kb on + strand at 4090.395 kb on + strand at 4090.396 kb on - strand at 4090.396 kb on - strand at 4090.399 kb on + strand at 4090.400 kb on - strand at 4090.404 kb on - strand at 4090.435 kb on + strand at 4090.494 kb on + strand at 4090.495 kb on - strand at 4090.498 kb on + strand at 4090.499 kb on - strand at 4090.500 kb on + strand at 4090.652 kb on + strand at 4090.653 kb on - strand at 4090.653 kb on - strand at 4090.780 kb on - strand, within pflA at 4091.163 kb on - strand, within pflA at 4091.242 kb on - strand, within pflA at 4091.267 kb on + strand at 4091.310 kb on + strand at 4091.324 kb on - strand at 4091.331 kb on + strand at 4091.416 kb on + strand at 4091.419 kb on - strand at 4091.427 kb on + strand at 4091.428 kb on - strand at 4091.428 kb on - strand at 4091.449 kb on - strand at 4091.652 kb on + strand, within ycaK at 4091.692 kb on - strand, within ycaK at 4091.702 kb on + strand, within ycaK at 4091.703 kb on - strand, within ycaK at 4091.703 kb on - strand, within ycaK at 4091.713 kb on - strand, within ycaK at 4091.901 kb on + strand, within ycaK at 4091.942 kb on + strand, within ycaK at 4091.942 kb on + strand, within ycaK at 4091.986 kb on + strand at 4091.990 kb on + strand at 4092.062 kb on + strand at 4092.216 kb on - strand, within ycaN at 4092.218 kb on + strand, within ycaN at 4092.219 kb on - strand, within ycaN at 4092.251 kb on - strand, within ycaN at 4092.297 kb on - strand, within ycaN at 4092.297 kb on - strand, within ycaN
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 4,089,617 - pflB BNILDI_20325 0.66 -0.0 4,089,632 - pflB BNILDI_20325 0.66 -0.9 4,089,840 - pflB BNILDI_20325 0.75 +0.0 4,089,946 - pflB BNILDI_20325 0.80 -1.1 4,089,946 - pflB BNILDI_20325 0.80 +0.6 4,089,956 - pflB BNILDI_20325 0.80 +0.1 4,089,956 - pflB BNILDI_20325 0.80 +0.1 4,089,956 - pflB BNILDI_20325 0.80 -0.7 4,089,960 + pflB BNILDI_20325 0.81 +0.7 4,090,093 + pflB BNILDI_20325 0.86 +0.5 4,090,107 + pflB BNILDI_20325 0.87 +0.2 4,090,114 + pflB BNILDI_20325 0.87 -0.2 4,090,196 - -0.1 4,090,375 - +0.3 4,090,380 + +0.7 4,090,395 + -0.0 4,090,396 - -1.2 4,090,396 - +1.5 4,090,399 + +1.2 4,090,400 - -0.9 4,090,404 - -0.4 4,090,435 + +0.3 4,090,494 + -0.7 4,090,495 - -0.0 4,090,498 + +0.3 4,090,499 - -0.4 4,090,500 + +0.1 4,090,652 + +0.4 4,090,653 - +1.0 4,090,653 - +0.2 4,090,780 - pflA BNILDI_20330 0.25 -1.5 4,091,163 - pflA BNILDI_20330 0.77 +0.9 4,091,242 - pflA BNILDI_20330 0.88 -0.7 4,091,267 + +2.1 4,091,310 + -1.4 4,091,324 - -0.7 4,091,331 + +0.4 4,091,416 + +1.0 4,091,419 - +0.4 4,091,427 + -0.0 4,091,428 - -0.2 4,091,428 - +0.5 4,091,449 - +0.6 4,091,652 + ycaK BNILDI_20335 0.40 -0.2 4,091,692 - ycaK BNILDI_20335 0.47 +1.6 4,091,702 + ycaK BNILDI_20335 0.49 +0.2 4,091,703 - ycaK BNILDI_20335 0.49 -0.4 4,091,703 - ycaK BNILDI_20335 0.49 -0.8 4,091,713 - ycaK BNILDI_20335 0.50 -0.9 4,091,901 + ycaK BNILDI_20335 0.82 -0.8 4,091,942 + ycaK BNILDI_20335 0.89 +0.4 4,091,942 + ycaK BNILDI_20335 0.89 -0.4 4,091,986 + +0.0 4,091,990 + -0.5 4,092,062 + -1.5 4,092,216 - ycaN BNILDI_20340 0.12 -0.0 4,092,218 + ycaN BNILDI_20340 0.12 +0.7 4,092,219 - ycaN BNILDI_20340 0.13 -1.0 4,092,251 - ycaN BNILDI_20340 0.16 -0.5 4,092,297 - ycaN BNILDI_20340 0.21 +0.2 4,092,297 - ycaN BNILDI_20340 0.21 -0.9
Or see this region's nucleotide sequence