Strain Fitness in Escherichia coli ECRC62 around BNILDI_20280

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycaI and ihfB are separated by 206 nucleotidesihfB and rpsA are separated by 159 nucleotides BNILDI_20275: ycaI - ComEC family protein, at 4,075,090 to 4,077,354 ycaI BNILDI_20280: ihfB - integration host factor subunit beta, at 4,077,561 to 4,077,845 ihfB BNILDI_20285: rpsA - 30S ribosomal protein S1, at 4,078,005 to 4,079,678 rpsA Position (kb) 4077 4078Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4076.609 kb on + strand, within ycaIat 4076.712 kb on + strand, within ycaIat 4076.781 kb on + strand, within ycaIat 4076.784 kb on - strand, within ycaIat 4076.832 kb on + strand, within ycaIat 4076.929 kb on - strand, within ycaIat 4076.929 kb on - strand, within ycaIat 4077.039 kb on + strand, within ycaIat 4077.039 kb on + strand, within ycaIat 4077.040 kb on - strand, within ycaIat 4077.040 kb on - strand, within ycaIat 4077.040 kb on - strand, within ycaIat 4077.040 kb on - strand, within ycaIat 4077.040 kb on - strand, within ycaIat 4077.052 kb on + strand, within ycaIat 4077.053 kb on - strand, within ycaIat 4077.061 kb on - strand, within ycaIat 4077.071 kb on + strand, within ycaIat 4077.134 kb on - strandat 4077.170 kb on - strandat 4077.172 kb on + strandat 4077.173 kb on - strandat 4077.209 kb on + strandat 4077.210 kb on - strandat 4077.325 kb on + strandat 4077.326 kb on - strandat 4077.326 kb on - strandat 4077.346 kb on + strandat 4077.569 kb on - strandat 4077.605 kb on + strand, within ihfBat 4078.017 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,076,609 + ycaI BNILDI_20275 0.67 -0.1
4,076,712 + ycaI BNILDI_20275 0.72 -0.2
4,076,781 + ycaI BNILDI_20275 0.75 +0.5
4,076,784 - ycaI BNILDI_20275 0.75 -0.1
4,076,832 + ycaI BNILDI_20275 0.77 +0.0
4,076,929 - ycaI BNILDI_20275 0.81 -1.1
4,076,929 - ycaI BNILDI_20275 0.81 +2.8
4,077,039 + ycaI BNILDI_20275 0.86 -2.5
4,077,039 + ycaI BNILDI_20275 0.86 -0.8
4,077,040 - ycaI BNILDI_20275 0.86 -1.2
4,077,040 - ycaI BNILDI_20275 0.86 +0.2
4,077,040 - ycaI BNILDI_20275 0.86 -0.0
4,077,040 - ycaI BNILDI_20275 0.86 +0.5
4,077,040 - ycaI BNILDI_20275 0.86 -0.5
4,077,052 + ycaI BNILDI_20275 0.87 -0.8
4,077,053 - ycaI BNILDI_20275 0.87 -0.1
4,077,061 - ycaI BNILDI_20275 0.87 -0.1
4,077,071 + ycaI BNILDI_20275 0.87 -0.3
4,077,134 - +1.3
4,077,170 - +0.2
4,077,172 + -0.4
4,077,173 - +0.2
4,077,209 + -0.1
4,077,210 - -1.5
4,077,325 + +0.1
4,077,326 - +0.9
4,077,326 - +0.2
4,077,346 + -0.3
4,077,569 - -1.1
4,077,605 + ihfB BNILDI_20280 0.15 -0.1
4,078,017 - -1.1

Or see this region's nucleotide sequence