Strain Fitness in Escherichia coli ECRC62 around BNILDI_20175

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntssuC and ssuB overlap by 4 nucleotidesssuB and pepN are separated by 206 nucleotidespepN and pncB are separated by 265 nucleotides BNILDI_20160: ssuC - aliphatic sulfonate ABC transporter permease SsuC, at 4,046,678 to 4,047,469 ssuC BNILDI_20165: ssuB - aliphatic sulfonates ABC transporter ATP-binding protein, at 4,047,466 to 4,048,233 ssuB BNILDI_20175: pepN - aminopeptidase N, at 4,048,440 to 4,051,052 pepN BNILDI_20180: pncB - nicotinate phosphoribosyltransferase, at 4,051,318 to 4,052,520 pncB Position (kb) 4048 4049 4050 4051 4052Strain fitness (log2 ratio) -2 -1 0 1 2at 4047.444 kb on - strandat 4047.465 kb on + strandat 4047.472 kb on - strandat 4047.526 kb on + strandat 4047.560 kb on + strand, within ssuBat 4047.561 kb on - strand, within ssuBat 4047.565 kb on - strand, within ssuBat 4047.740 kb on + strand, within ssuBat 4047.923 kb on + strand, within ssuBat 4047.924 kb on - strand, within ssuBat 4048.079 kb on + strand, within ssuBat 4048.279 kb on - strandat 4048.439 kb on - strandat 4048.468 kb on + strandat 4048.546 kb on + strandat 4048.709 kb on + strand, within pepNat 4048.730 kb on + strand, within pepNat 4048.731 kb on - strand, within pepNat 4048.731 kb on - strand, within pepNat 4048.842 kb on - strand, within pepNat 4049.030 kb on + strand, within pepNat 4049.031 kb on - strand, within pepNat 4049.074 kb on + strand, within pepNat 4049.074 kb on + strand, within pepNat 4049.078 kb on + strand, within pepNat 4049.330 kb on - strand, within pepNat 4049.330 kb on - strand, within pepNat 4049.421 kb on - strand, within pepNat 4049.423 kb on - strand, within pepNat 4049.585 kb on - strand, within pepNat 4049.692 kb on + strand, within pepNat 4049.692 kb on + strand, within pepNat 4049.734 kb on + strand, within pepNat 4049.734 kb on + strand, within pepNat 4049.926 kb on + strand, within pepNat 4049.926 kb on + strand, within pepNat 4050.029 kb on + strand, within pepNat 4050.029 kb on + strand, within pepNat 4050.030 kb on - strand, within pepNat 4050.229 kb on + strand, within pepNat 4050.240 kb on - strand, within pepNat 4050.257 kb on + strand, within pepNat 4050.303 kb on - strand, within pepNat 4050.305 kb on - strand, within pepNat 4050.305 kb on - strand, within pepNat 4050.305 kb on - strand, within pepNat 4050.305 kb on - strand, within pepNat 4050.320 kb on - strand, within pepNat 4050.413 kb on - strand, within pepNat 4050.450 kb on - strand, within pepNat 4050.465 kb on + strand, within pepNat 4050.465 kb on + strand, within pepNat 4050.598 kb on + strand, within pepNat 4050.661 kb on + strand, within pepNat 4050.662 kb on - strand, within pepNat 4050.664 kb on + strand, within pepNat 4050.668 kb on + strand, within pepNat 4050.668 kb on + strand, within pepNat 4050.669 kb on - strand, within pepNat 4050.669 kb on - strand, within pepNat 4050.669 kb on - strand, within pepNat 4050.760 kb on - strand, within pepNat 4050.776 kb on + strand, within pepNat 4050.854 kb on + strandat 4050.855 kb on - strandat 4050.856 kb on + strandat 4050.857 kb on + strandat 4050.993 kb on - strandat 4051.052 kb on + strandat 4051.056 kb on + strandat 4051.056 kb on + strandat 4051.073 kb on + strandat 4051.073 kb on + strandat 4051.074 kb on - strandat 4051.076 kb on - strandat 4051.108 kb on - strandat 4051.168 kb on + strandat 4051.169 kb on - strandat 4051.176 kb on - strandat 4051.210 kb on + strandat 4051.211 kb on - strandat 4051.232 kb on - strandat 4051.232 kb on - strandat 4051.249 kb on - strandat 4051.249 kb on - strandat 4051.275 kb on + strandat 4051.317 kb on + strandat 4051.318 kb on - strandat 4051.370 kb on - strandat 4051.370 kb on - strandat 4051.371 kb on + strandat 4051.372 kb on - strandat 4051.380 kb on + strandat 4051.406 kb on - strandat 4051.408 kb on + strandat 4051.408 kb on + strandat 4051.461 kb on + strand, within pncBat 4051.466 kb on - strand, within pncBat 4051.466 kb on - strand, within pncBat 4051.476 kb on + strand, within pncBat 4051.477 kb on - strand, within pncBat 4051.529 kb on - strand, within pncBat 4051.529 kb on - strand, within pncBat 4051.572 kb on + strand, within pncBat 4051.572 kb on + strand, within pncBat 4051.812 kb on + strand, within pncBat 4051.813 kb on - strand, within pncBat 4051.869 kb on - strand, within pncBat 4051.869 kb on - strand, within pncBat 4051.927 kb on + strand, within pncBat 4051.928 kb on - strand, within pncBat 4051.928 kb on - strand, within pncBat 4052.015 kb on + strand, within pncB

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,047,444 - +2.5
4,047,465 + -0.7
4,047,472 - +0.4
4,047,526 + +0.5
4,047,560 + ssuB BNILDI_20165 0.12 +1.9
4,047,561 - ssuB BNILDI_20165 0.12 +0.8
4,047,565 - ssuB BNILDI_20165 0.13 -0.8
4,047,740 + ssuB BNILDI_20165 0.36 -0.3
4,047,923 + ssuB BNILDI_20165 0.60 -0.3
4,047,924 - ssuB BNILDI_20165 0.60 +0.3
4,048,079 + ssuB BNILDI_20165 0.80 +0.4
4,048,279 - +0.9
4,048,439 - +0.2
4,048,468 + -0.1
4,048,546 + +0.1
4,048,709 + pepN BNILDI_20175 0.10 -0.7
4,048,730 + pepN BNILDI_20175 0.11 +0.9
4,048,731 - pepN BNILDI_20175 0.11 -0.9
4,048,731 - pepN BNILDI_20175 0.11 -1.3
4,048,842 - pepN BNILDI_20175 0.15 -0.3
4,049,030 + pepN BNILDI_20175 0.23 +1.0
4,049,031 - pepN BNILDI_20175 0.23 -0.0
4,049,074 + pepN BNILDI_20175 0.24 +0.2
4,049,074 + pepN BNILDI_20175 0.24 -0.0
4,049,078 + pepN BNILDI_20175 0.24 -1.1
4,049,330 - pepN BNILDI_20175 0.34 -0.1
4,049,330 - pepN BNILDI_20175 0.34 +0.4
4,049,421 - pepN BNILDI_20175 0.38 -0.1
4,049,423 - pepN BNILDI_20175 0.38 +1.2
4,049,585 - pepN BNILDI_20175 0.44 +1.2
4,049,692 + pepN BNILDI_20175 0.48 +0.3
4,049,692 + pepN BNILDI_20175 0.48 -0.1
4,049,734 + pepN BNILDI_20175 0.50 +0.7
4,049,734 + pepN BNILDI_20175 0.50 +0.8
4,049,926 + pepN BNILDI_20175 0.57 +0.2
4,049,926 + pepN BNILDI_20175 0.57 -2.2
4,050,029 + pepN BNILDI_20175 0.61 +0.0
4,050,029 + pepN BNILDI_20175 0.61 -1.9
4,050,030 - pepN BNILDI_20175 0.61 +1.1
4,050,229 + pepN BNILDI_20175 0.68 -1.1
4,050,240 - pepN BNILDI_20175 0.69 -1.0
4,050,257 + pepN BNILDI_20175 0.70 +0.3
4,050,303 - pepN BNILDI_20175 0.71 +0.3
4,050,305 - pepN BNILDI_20175 0.71 +1.2
4,050,305 - pepN BNILDI_20175 0.71 +0.2
4,050,305 - pepN BNILDI_20175 0.71 -0.3
4,050,305 - pepN BNILDI_20175 0.71 +0.4
4,050,320 - pepN BNILDI_20175 0.72 -0.4
4,050,413 - pepN BNILDI_20175 0.76 -0.1
4,050,450 - pepN BNILDI_20175 0.77 -0.7
4,050,465 + pepN BNILDI_20175 0.77 -0.2
4,050,465 + pepN BNILDI_20175 0.77 -0.1
4,050,598 + pepN BNILDI_20175 0.83 +1.5
4,050,661 + pepN BNILDI_20175 0.85 +1.2
4,050,662 - pepN BNILDI_20175 0.85 +0.2
4,050,664 + pepN BNILDI_20175 0.85 +0.1
4,050,668 + pepN BNILDI_20175 0.85 -1.1
4,050,668 + pepN BNILDI_20175 0.85 +0.5
4,050,669 - pepN BNILDI_20175 0.85 -0.5
4,050,669 - pepN BNILDI_20175 0.85 +0.1
4,050,669 - pepN BNILDI_20175 0.85 -0.0
4,050,760 - pepN BNILDI_20175 0.89 +0.4
4,050,776 + pepN BNILDI_20175 0.89 -0.0
4,050,854 + -0.3
4,050,855 - +0.3
4,050,856 + -1.4
4,050,857 + -0.6
4,050,993 - +0.1
4,051,052 + -0.3
4,051,056 + -0.6
4,051,056 + +0.3
4,051,073 + +0.6
4,051,073 + -0.1
4,051,074 - -0.0
4,051,076 - +1.9
4,051,108 - +0.1
4,051,168 + -0.0
4,051,169 - +0.0
4,051,176 - -0.2
4,051,210 + +0.6
4,051,211 - -0.1
4,051,232 - +1.0
4,051,232 - +1.3
4,051,249 - +0.4
4,051,249 - -0.1
4,051,275 + -0.9
4,051,317 + -0.5
4,051,318 - +0.6
4,051,370 - -0.7
4,051,370 - +1.4
4,051,371 + +0.8
4,051,372 - -0.2
4,051,380 + +0.3
4,051,406 - +1.5
4,051,408 + -0.5
4,051,408 + +0.6
4,051,461 + pncB BNILDI_20180 0.12 -1.0
4,051,466 - pncB BNILDI_20180 0.12 -0.7
4,051,466 - pncB BNILDI_20180 0.12 +0.6
4,051,476 + pncB BNILDI_20180 0.13 -0.6
4,051,477 - pncB BNILDI_20180 0.13 +0.8
4,051,529 - pncB BNILDI_20180 0.18 -0.0
4,051,529 - pncB BNILDI_20180 0.18 -0.7
4,051,572 + pncB BNILDI_20180 0.21 +0.9
4,051,572 + pncB BNILDI_20180 0.21 +0.9
4,051,812 + pncB BNILDI_20180 0.41 -0.1
4,051,813 - pncB BNILDI_20180 0.41 -0.7
4,051,869 - pncB BNILDI_20180 0.46 -0.5
4,051,869 - pncB BNILDI_20180 0.46 -0.1
4,051,927 + pncB BNILDI_20180 0.51 -1.9
4,051,928 - pncB BNILDI_20180 0.51 -0.9
4,051,928 - pncB BNILDI_20180 0.51 -1.6
4,052,015 + pncB BNILDI_20180 0.58 +0.7

Or see this region's nucleotide sequence