Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rutE and rutF are separated by 10 nucleotides rutF and rutG are separated by 20 nucleotides rutG and BNILDI_19715 are separated by 82 nucleotides BNILDI_19715 and wrbA are separated by 372 nucleotides
BNILDI_19700: rutE - malonic semialdehyde reductase, at 3,954,322 to 3,954,912
rutE
BNILDI_19705: rutF - malonic semialdehyde reductase, at 3,954,923 to 3,955,417
rutF
BNILDI_19710: rutG - pyrimidine utilization transport protein G, at 3,955,438 to 3,956,766
rutG
BNILDI_19715: BNILDI_19715 - Stress-induced protein, KGG, repeat, at 3,956,849 to 3,956,956
_19715
BNILDI_19720: wrbA - NAD(P)H:quinone oxidoreductase, at 3,957,329 to 3,957,925
wrbA
Position (kb)
3955
3956
3957 Strain fitness (log2 ratio)
-2
-1
0
1 at 3954.500 kb on + strand, within rutE at 3954.617 kb on + strand, within rutE at 3954.652 kb on - strand, within rutE at 3954.922 kb on + strand at 3954.923 kb on - strand at 3954.930 kb on + strand at 3954.930 kb on + strand at 3954.930 kb on + strand at 3954.930 kb on + strand at 3954.930 kb on + strand at 3954.931 kb on - strand at 3955.069 kb on - strand, within rutF at 3955.470 kb on - strand at 3955.625 kb on + strand, within rutG at 3955.659 kb on + strand, within rutG at 3955.804 kb on + strand, within rutG at 3955.807 kb on - strand, within rutG at 3956.045 kb on + strand, within rutG at 3956.045 kb on + strand, within rutG at 3956.045 kb on + strand, within rutG at 3956.046 kb on - strand, within rutG at 3956.087 kb on + strand, within rutG at 3956.169 kb on + strand, within rutG at 3956.169 kb on + strand, within rutG at 3956.169 kb on + strand, within rutG at 3956.172 kb on + strand, within rutG at 3956.228 kb on + strand, within rutG at 3956.229 kb on - strand, within rutG at 3956.278 kb on + strand, within rutG at 3956.279 kb on - strand, within rutG at 3956.279 kb on - strand, within rutG at 3956.280 kb on + strand, within rutG at 3956.357 kb on - strand, within rutG at 3956.475 kb on + strand, within rutG at 3956.655 kb on + strand at 3956.711 kb on + strand at 3956.977 kb on + strand at 3956.977 kb on + strand at 3956.978 kb on - strand at 3956.987 kb on - strand at 3956.987 kb on - strand at 3956.993 kb on + strand at 3956.993 kb on + strand at 3956.994 kb on - strand at 3956.999 kb on + strand at 3957.023 kb on + strand at 3957.043 kb on - strand at 3957.043 kb on - strand at 3957.049 kb on - strand at 3957.114 kb on + strand at 3957.268 kb on - strand at 3957.278 kb on - strand at 3957.278 kb on - strand at 3957.278 kb on - strand at 3957.303 kb on - strand at 3957.309 kb on - strand at 3957.351 kb on - strand at 3957.353 kb on + strand at 3957.353 kb on + strand at 3957.362 kb on + strand at 3957.363 kb on - strand at 3957.370 kb on + strand at 3957.370 kb on + strand at 3957.636 kb on + strand, within wrbA at 3957.661 kb on + strand, within wrbA at 3957.661 kb on + strand, within wrbA at 3957.662 kb on - strand, within wrbA at 3957.697 kb on + strand, within wrbA at 3957.697 kb on + strand, within wrbA at 3957.697 kb on + strand, within wrbA at 3957.700 kb on + strand, within wrbA at 3957.701 kb on - strand, within wrbA at 3957.738 kb on + strand, within wrbA
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 3,954,500 + rutE BNILDI_19700 0.30 -0.5 3,954,617 + rutE BNILDI_19700 0.50 -0.1 3,954,652 - rutE BNILDI_19700 0.56 -0.5 3,954,922 + +0.9 3,954,923 - -0.5 3,954,930 + -0.2 3,954,930 + +0.8 3,954,930 + -0.2 3,954,930 + +0.4 3,954,930 + -0.9 3,954,931 - -0.2 3,955,069 - rutF BNILDI_19705 0.29 +0.0 3,955,470 - +0.0 3,955,625 + rutG BNILDI_19710 0.14 +0.4 3,955,659 + rutG BNILDI_19710 0.17 -0.8 3,955,804 + rutG BNILDI_19710 0.28 +0.3 3,955,807 - rutG BNILDI_19710 0.28 +0.2 3,956,045 + rutG BNILDI_19710 0.46 -0.1 3,956,045 + rutG BNILDI_19710 0.46 +0.3 3,956,045 + rutG BNILDI_19710 0.46 -0.7 3,956,046 - rutG BNILDI_19710 0.46 -0.5 3,956,087 + rutG BNILDI_19710 0.49 +0.8 3,956,169 + rutG BNILDI_19710 0.55 +0.7 3,956,169 + rutG BNILDI_19710 0.55 -0.2 3,956,169 + rutG BNILDI_19710 0.55 -0.1 3,956,172 + rutG BNILDI_19710 0.55 +0.0 3,956,228 + rutG BNILDI_19710 0.59 +0.0 3,956,229 - rutG BNILDI_19710 0.60 +1.6 3,956,278 + rutG BNILDI_19710 0.63 +0.8 3,956,279 - rutG BNILDI_19710 0.63 -2.2 3,956,279 - rutG BNILDI_19710 0.63 +0.4 3,956,280 + rutG BNILDI_19710 0.63 +0.1 3,956,357 - rutG BNILDI_19710 0.69 -0.0 3,956,475 + rutG BNILDI_19710 0.78 -0.0 3,956,655 + -0.1 3,956,711 + -0.2 3,956,977 + +0.8 3,956,977 + -0.1 3,956,978 - +0.1 3,956,987 - +0.5 3,956,987 - +0.2 3,956,993 + -0.3 3,956,993 + -0.6 3,956,994 - -0.2 3,956,999 + -1.3 3,957,023 + +0.1 3,957,043 - -1.7 3,957,043 - +0.2 3,957,049 - +0.6 3,957,114 + +1.3 3,957,268 - -0.7 3,957,278 - +0.1 3,957,278 - -0.6 3,957,278 - -2.2 3,957,303 - +0.2 3,957,309 - -1.6 3,957,351 - -0.1 3,957,353 + -0.3 3,957,353 + -0.4 3,957,362 + +0.4 3,957,363 - -0.0 3,957,370 + +0.8 3,957,370 + +1.3 3,957,636 + wrbA BNILDI_19720 0.51 +0.9 3,957,661 + wrbA BNILDI_19720 0.56 -0.1 3,957,661 + wrbA BNILDI_19720 0.56 -1.1 3,957,662 - wrbA BNILDI_19720 0.56 +0.4 3,957,697 + wrbA BNILDI_19720 0.62 +0.2 3,957,697 + wrbA BNILDI_19720 0.62 +0.4 3,957,697 + wrbA BNILDI_19720 0.62 +1.0 3,957,700 + wrbA BNILDI_19720 0.62 -0.7 3,957,701 - wrbA BNILDI_19720 0.62 +0.6 3,957,738 + wrbA BNILDI_19720 0.69 -2.5
Or see this region's nucleotide sequence