Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sirB1 and sirB2 are separated by 3 nucleotides sirB2 and prmC overlap by 4 nucleotides prmC and prfA overlap by 1 nucleotides
BNILDI_18750: sirB1 - invasion regulator SirB1, at 3,771,112 to 3,771,921
sirB1
BNILDI_18755: sirB2 - invasion regulator SirB2, at 3,771,925 to 3,772,317
sirB2
BNILDI_18760: prmC - peptide chain release factor N(5)-glutamine methyltransferase, at 3,772,314 to 3,773,147
prmC
BNILDI_18765: prfA - peptide chain release factor 1, at 3,773,147 to 3,774,229
prfA
Position (kb)
3772
3773
3774 Strain fitness (log2 ratio)
-2
-1
0
1 at 3771.420 kb on - strand, within sirB1 at 3771.444 kb on + strand, within sirB1 at 3771.460 kb on + strand, within sirB1 at 3771.461 kb on - strand, within sirB1 at 3771.461 kb on - strand, within sirB1 at 3771.471 kb on + strand, within sirB1 at 3771.496 kb on - strand, within sirB1 at 3771.504 kb on + strand, within sirB1 at 3771.619 kb on + strand, within sirB1 at 3771.620 kb on + strand, within sirB1 at 3771.706 kb on - strand, within sirB1 at 3771.892 kb on - strand at 3771.926 kb on + strand at 3771.927 kb on - strand at 3771.960 kb on + strand at 3771.971 kb on + strand, within sirB2 at 3771.972 kb on - strand, within sirB2 at 3772.026 kb on + strand, within sirB2 at 3772.071 kb on + strand, within sirB2 at 3772.252 kb on + strand, within sirB2 at 3772.253 kb on - strand, within sirB2 at 3772.256 kb on - strand, within sirB2 at 3772.282 kb on - strand at 3772.282 kb on - strand at 3772.282 kb on - strand at 3772.294 kb on - strand at 3772.294 kb on - strand at 3772.321 kb on + strand at 3772.322 kb on - strand at 3772.694 kb on + strand, within prmC at 3772.724 kb on + strand, within prmC at 3772.882 kb on - strand, within prmC at 3773.033 kb on + strand, within prmC at 3773.115 kb on + strand at 3773.128 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 3,771,420 - sirB1 BNILDI_18750 0.38 +0.2 3,771,444 + sirB1 BNILDI_18750 0.41 +1.0 3,771,460 + sirB1 BNILDI_18750 0.43 +1.6 3,771,461 - sirB1 BNILDI_18750 0.43 -0.9 3,771,461 - sirB1 BNILDI_18750 0.43 -0.6 3,771,471 + sirB1 BNILDI_18750 0.44 -1.0 3,771,496 - sirB1 BNILDI_18750 0.47 +0.2 3,771,504 + sirB1 BNILDI_18750 0.48 +0.4 3,771,619 + sirB1 BNILDI_18750 0.63 +0.4 3,771,620 + sirB1 BNILDI_18750 0.63 -1.8 3,771,706 - sirB1 BNILDI_18750 0.73 -0.2 3,771,892 - +0.3 3,771,926 + -0.8 3,771,927 - -1.3 3,771,960 + -0.1 3,771,971 + sirB2 BNILDI_18755 0.12 +0.4 3,771,972 - sirB2 BNILDI_18755 0.12 -0.1 3,772,026 + sirB2 BNILDI_18755 0.26 -0.8 3,772,071 + sirB2 BNILDI_18755 0.37 -1.3 3,772,252 + sirB2 BNILDI_18755 0.83 -0.3 3,772,253 - sirB2 BNILDI_18755 0.83 +0.7 3,772,256 - sirB2 BNILDI_18755 0.84 -0.6 3,772,282 - -0.7 3,772,282 - -0.1 3,772,282 - -0.2 3,772,294 - -1.3 3,772,294 - +0.4 3,772,321 + -1.3 3,772,322 - -0.7 3,772,694 + prmC BNILDI_18760 0.46 -0.7 3,772,724 + prmC BNILDI_18760 0.49 -1.7 3,772,882 - prmC BNILDI_18760 0.68 -1.3 3,773,033 + prmC BNILDI_18760 0.86 -1.4 3,773,115 + +1.5 3,773,128 - -0.7
Or see this region's nucleotide sequence