Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvB and znuB are separated by 146 nucleotides znuB and znuC overlap by 4 nucleotides znuC and znuA are separated by 24 nucleotides
BNILDI_15520: ruvB - Holliday junction branch migration DNA helicase RuvB, at 3,116,509 to 3,117,519
ruvB
BNILDI_15525: znuB - zinc ABC transporter permease subunit ZnuB, at 3,117,666 to 3,118,451
znuB
BNILDI_15530: znuC - zinc ABC transporter ATP-binding protein ZnuC, at 3,118,448 to 3,119,203
znuC
BNILDI_15535: znuA - zinc ABC transporter substrate-binding protein ZnuA, at 3,119,228 to 3,120,214
znuA
Position (kb)
3117
3118
3119 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3116.887 kb on + strand, within ruvB at 3117.081 kb on + strand, within ruvB at 3117.614 kb on + strand at 3117.667 kb on + strand at 3117.668 kb on - strand at 3117.668 kb on - strand at 3117.668 kb on - strand at 3117.718 kb on - strand at 3117.879 kb on - strand, within znuB at 3118.034 kb on + strand, within znuB at 3118.137 kb on - strand, within znuB at 3118.279 kb on + strand, within znuB at 3118.324 kb on - strand, within znuB at 3118.344 kb on - strand, within znuB at 3118.355 kb on - strand, within znuB at 3118.441 kb on - strand at 3118.558 kb on + strand, within znuC at 3118.564 kb on + strand, within znuC at 3118.578 kb on - strand, within znuC at 3118.725 kb on + strand, within znuC at 3118.725 kb on + strand, within znuC at 3118.726 kb on - strand, within znuC at 3118.727 kb on + strand, within znuC at 3118.728 kb on - strand, within znuC at 3118.745 kb on + strand, within znuC at 3118.746 kb on - strand, within znuC at 3118.814 kb on + strand, within znuC at 3118.815 kb on - strand, within znuC at 3118.818 kb on - strand, within znuC at 3118.913 kb on - strand, within znuC at 3118.975 kb on - strand, within znuC at 3118.989 kb on - strand, within znuC at 3118.992 kb on + strand, within znuC at 3118.993 kb on - strand, within znuC at 3118.993 kb on - strand, within znuC at 3119.077 kb on + strand, within znuC at 3119.123 kb on - strand, within znuC at 3119.287 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 3,116,887 + ruvB BNILDI_15520 0.37 -3.5 3,117,081 + ruvB BNILDI_15520 0.57 -1.9 3,117,614 + +1.1 3,117,667 + -0.6 3,117,668 - -0.3 3,117,668 - +0.6 3,117,668 - +1.2 3,117,718 - -1.4 3,117,879 - znuB BNILDI_15525 0.27 -0.5 3,118,034 + znuB BNILDI_15525 0.47 -0.0 3,118,137 - znuB BNILDI_15525 0.60 -2.0 3,118,279 + znuB BNILDI_15525 0.78 -0.2 3,118,324 - znuB BNILDI_15525 0.84 -2.1 3,118,344 - znuB BNILDI_15525 0.86 -3.3 3,118,355 - znuB BNILDI_15525 0.88 -0.8 3,118,441 - -1.5 3,118,558 + znuC BNILDI_15530 0.15 -1.3 3,118,564 + znuC BNILDI_15530 0.15 +1.1 3,118,578 - znuC BNILDI_15530 0.17 +0.2 3,118,725 + znuC BNILDI_15530 0.37 -3.4 3,118,725 + znuC BNILDI_15530 0.37 +0.0 3,118,726 - znuC BNILDI_15530 0.37 -0.7 3,118,727 + znuC BNILDI_15530 0.37 +0.4 3,118,728 - znuC BNILDI_15530 0.37 -0.7 3,118,745 + znuC BNILDI_15530 0.39 -0.6 3,118,746 - znuC BNILDI_15530 0.39 -0.8 3,118,814 + znuC BNILDI_15530 0.48 -1.0 3,118,815 - znuC BNILDI_15530 0.49 -0.7 3,118,818 - znuC BNILDI_15530 0.49 +0.1 3,118,913 - znuC BNILDI_15530 0.62 -3.0 3,118,975 - znuC BNILDI_15530 0.70 -2.9 3,118,989 - znuC BNILDI_15530 0.72 -1.3 3,118,992 + znuC BNILDI_15530 0.72 -1.1 3,118,993 - znuC BNILDI_15530 0.72 -1.8 3,118,993 - znuC BNILDI_15530 0.72 -2.4 3,119,077 + znuC BNILDI_15530 0.83 -1.9 3,119,123 - znuC BNILDI_15530 0.89 -2.1 3,119,287 - +0.0
Or see this region's nucleotide sequence