Strain Fitness in Escherichia coli ECRC62 around BNILDI_15525

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntruvB and znuB are separated by 146 nucleotidesznuB and znuC overlap by 4 nucleotidesznuC and znuA are separated by 24 nucleotides BNILDI_15520: ruvB - Holliday junction branch migration DNA helicase RuvB, at 3,116,509 to 3,117,519 ruvB BNILDI_15525: znuB - zinc ABC transporter permease subunit ZnuB, at 3,117,666 to 3,118,451 znuB BNILDI_15530: znuC - zinc ABC transporter ATP-binding protein ZnuC, at 3,118,448 to 3,119,203 znuC BNILDI_15535: znuA - zinc ABC transporter substrate-binding protein ZnuA, at 3,119,228 to 3,120,214 znuA Position (kb) 3117 3118 3119Strain fitness (log2 ratio) -3 -2 -1 0 1at 3116.887 kb on + strand, within ruvBat 3117.081 kb on + strand, within ruvBat 3117.614 kb on + strandat 3117.667 kb on + strandat 3117.668 kb on - strandat 3117.668 kb on - strandat 3117.668 kb on - strandat 3117.718 kb on - strandat 3117.879 kb on - strand, within znuBat 3118.034 kb on + strand, within znuBat 3118.137 kb on - strand, within znuBat 3118.279 kb on + strand, within znuBat 3118.324 kb on - strand, within znuBat 3118.344 kb on - strand, within znuBat 3118.355 kb on - strand, within znuBat 3118.441 kb on - strandat 3118.558 kb on + strand, within znuCat 3118.564 kb on + strand, within znuCat 3118.578 kb on - strand, within znuCat 3118.725 kb on + strand, within znuCat 3118.725 kb on + strand, within znuCat 3118.726 kb on - strand, within znuCat 3118.727 kb on + strand, within znuCat 3118.728 kb on - strand, within znuCat 3118.745 kb on + strand, within znuCat 3118.746 kb on - strand, within znuCat 3118.814 kb on + strand, within znuCat 3118.815 kb on - strand, within znuCat 3118.818 kb on - strand, within znuCat 3118.913 kb on - strand, within znuCat 3118.975 kb on - strand, within znuCat 3118.989 kb on - strand, within znuCat 3118.992 kb on + strand, within znuCat 3118.993 kb on - strand, within znuCat 3118.993 kb on - strand, within znuCat 3119.077 kb on + strand, within znuCat 3119.123 kb on - strand, within znuCat 3119.287 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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3,116,887 + ruvB BNILDI_15520 0.37 -3.5
3,117,081 + ruvB BNILDI_15520 0.57 -1.9
3,117,614 + +1.1
3,117,667 + -0.6
3,117,668 - -0.3
3,117,668 - +0.6
3,117,668 - +1.2
3,117,718 - -1.4
3,117,879 - znuB BNILDI_15525 0.27 -0.5
3,118,034 + znuB BNILDI_15525 0.47 -0.0
3,118,137 - znuB BNILDI_15525 0.60 -2.0
3,118,279 + znuB BNILDI_15525 0.78 -0.2
3,118,324 - znuB BNILDI_15525 0.84 -2.1
3,118,344 - znuB BNILDI_15525 0.86 -3.3
3,118,355 - znuB BNILDI_15525 0.88 -0.8
3,118,441 - -1.5
3,118,558 + znuC BNILDI_15530 0.15 -1.3
3,118,564 + znuC BNILDI_15530 0.15 +1.1
3,118,578 - znuC BNILDI_15530 0.17 +0.2
3,118,725 + znuC BNILDI_15530 0.37 -3.4
3,118,725 + znuC BNILDI_15530 0.37 +0.0
3,118,726 - znuC BNILDI_15530 0.37 -0.7
3,118,727 + znuC BNILDI_15530 0.37 +0.4
3,118,728 - znuC BNILDI_15530 0.37 -0.7
3,118,745 + znuC BNILDI_15530 0.39 -0.6
3,118,746 - znuC BNILDI_15530 0.39 -0.8
3,118,814 + znuC BNILDI_15530 0.48 -1.0
3,118,815 - znuC BNILDI_15530 0.49 -0.7
3,118,818 - znuC BNILDI_15530 0.49 +0.1
3,118,913 - znuC BNILDI_15530 0.62 -3.0
3,118,975 - znuC BNILDI_15530 0.70 -2.9
3,118,989 - znuC BNILDI_15530 0.72 -1.3
3,118,992 + znuC BNILDI_15530 0.72 -1.1
3,118,993 - znuC BNILDI_15530 0.72 -1.8
3,118,993 - znuC BNILDI_15530 0.72 -2.4
3,119,077 + znuC BNILDI_15530 0.83 -1.9
3,119,123 - znuC BNILDI_15530 0.89 -2.1
3,119,287 - +0.0

Or see this region's nucleotide sequence