Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisD and hisG are separated by 5 nucleotides hisG and hisL are separated by 145 nucleotides hisL and yeeZ are separated by 425 nucleotides
BNILDI_14740: hisD - Histidinol dehydrogenase, at 2,977,669 to 2,978,973
hisD
BNILDI_14745: hisG - ATP phosphoribosyltransferase, at 2,978,979 to 2,979,878
hisG
BNILDI_14750: hisL - his operon leader peptide, at 2,980,024 to 2,980,074
hisL
BNILDI_14755: yeeZ - Protein YeeZ, at 2,980,500 to 2,981,324
yeeZ
Position (kb)
2978
2979
2980 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2978.024 kb on - strand, within hisD at 2978.549 kb on - strand, within hisD at 2978.584 kb on - strand, within hisD at 2978.585 kb on + strand, within hisD at 2978.586 kb on - strand, within hisD at 2978.586 kb on - strand, within hisD at 2978.586 kb on - strand, within hisD at 2978.586 kb on - strand, within hisD at 2978.647 kb on + strand, within hisD at 2978.911 kb on + strand at 2978.938 kb on - strand at 2978.943 kb on + strand at 2978.943 kb on + strand at 2978.943 kb on + strand at 2979.090 kb on + strand, within hisG at 2979.196 kb on + strand, within hisG at 2979.440 kb on + strand, within hisG at 2979.466 kb on + strand, within hisG at 2979.500 kb on + strand, within hisG at 2979.501 kb on - strand, within hisG at 2979.596 kb on + strand, within hisG at 2979.602 kb on - strand, within hisG at 2979.602 kb on - strand, within hisG at 2979.788 kb on + strand, within hisG at 2979.828 kb on + strand at 2979.828 kb on + strand at 2979.853 kb on - strand at 2980.025 kb on + strand at 2980.074 kb on + strand at 2980.118 kb on - strand at 2980.153 kb on - strand at 2980.217 kb on + strand at 2980.217 kb on + strand at 2980.217 kb on + strand at 2980.218 kb on - strand at 2980.218 kb on - strand at 2980.218 kb on - strand at 2980.223 kb on - strand at 2980.230 kb on + strand at 2980.242 kb on + strand at 2980.281 kb on + strand at 2980.281 kb on + strand at 2980.401 kb on + strand at 2980.401 kb on + strand at 2980.403 kb on + strand at 2980.411 kb on - strand at 2980.431 kb on + strand at 2980.432 kb on - strand at 2980.453 kb on + strand at 2980.470 kb on - strand at 2980.471 kb on + strand at 2980.471 kb on + strand at 2980.472 kb on - strand at 2980.472 kb on - strand at 2980.477 kb on + strand at 2980.484 kb on + strand at 2980.638 kb on - strand, within yeeZ at 2980.638 kb on - strand, within yeeZ at 2980.641 kb on + strand, within yeeZ at 2980.642 kb on - strand, within yeeZ at 2980.666 kb on + strand, within yeeZ at 2980.666 kb on + strand, within yeeZ at 2980.722 kb on - strand, within yeeZ at 2980.739 kb on + strand, within yeeZ at 2980.761 kb on + strand, within yeeZ at 2980.765 kb on + strand, within yeeZ at 2980.765 kb on + strand, within yeeZ at 2980.775 kb on - strand, within yeeZ at 2980.808 kb on + strand, within yeeZ at 2980.820 kb on + strand, within yeeZ at 2980.877 kb on + strand, within yeeZ
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,978,024 - hisD BNILDI_14740 0.27 -5.2 2,978,549 - hisD BNILDI_14740 0.67 -2.5 2,978,584 - hisD BNILDI_14740 0.70 -3.7 2,978,585 + hisD BNILDI_14740 0.70 -3.9 2,978,586 - hisD BNILDI_14740 0.70 -1.5 2,978,586 - hisD BNILDI_14740 0.70 -1.6 2,978,586 - hisD BNILDI_14740 0.70 -3.4 2,978,586 - hisD BNILDI_14740 0.70 -2.5 2,978,647 + hisD BNILDI_14740 0.75 -6.4 2,978,911 + -5.1 2,978,938 - -1.4 2,978,943 + -5.4 2,978,943 + -4.6 2,978,943 + +0.8 2,979,090 + hisG BNILDI_14745 0.12 -4.2 2,979,196 + hisG BNILDI_14745 0.24 +0.8 2,979,440 + hisG BNILDI_14745 0.51 -5.1 2,979,466 + hisG BNILDI_14745 0.54 -3.6 2,979,500 + hisG BNILDI_14745 0.58 -2.9 2,979,501 - hisG BNILDI_14745 0.58 -3.1 2,979,596 + hisG BNILDI_14745 0.69 -2.3 2,979,602 - hisG BNILDI_14745 0.69 -1.5 2,979,602 - hisG BNILDI_14745 0.69 +0.2 2,979,788 + hisG BNILDI_14745 0.90 -3.3 2,979,828 + -3.0 2,979,828 + -5.3 2,979,853 - -4.0 2,980,025 + -4.1 2,980,074 + -3.7 2,980,118 - +0.2 2,980,153 - +0.0 2,980,217 + +0.1 2,980,217 + -0.3 2,980,217 + -0.7 2,980,218 - +1.1 2,980,218 - +0.1 2,980,218 - +0.1 2,980,223 - +0.2 2,980,230 + -0.6 2,980,242 + +0.8 2,980,281 + -0.9 2,980,281 + +0.5 2,980,401 + +0.5 2,980,401 + -0.2 2,980,403 + +0.4 2,980,411 - +0.6 2,980,431 + +0.7 2,980,432 - +0.6 2,980,453 + +0.7 2,980,470 - -1.0 2,980,471 + +0.2 2,980,471 + -0.5 2,980,472 - -0.0 2,980,472 - -0.2 2,980,477 + +0.1 2,980,484 + +0.2 2,980,638 - yeeZ BNILDI_14755 0.17 -0.7 2,980,638 - yeeZ BNILDI_14755 0.17 -1.0 2,980,641 + yeeZ BNILDI_14755 0.17 +1.6 2,980,642 - yeeZ BNILDI_14755 0.17 -0.1 2,980,666 + yeeZ BNILDI_14755 0.20 -0.9 2,980,666 + yeeZ BNILDI_14755 0.20 -2.0 2,980,722 - yeeZ BNILDI_14755 0.27 -0.6 2,980,739 + yeeZ BNILDI_14755 0.29 +0.0 2,980,761 + yeeZ BNILDI_14755 0.32 -0.1 2,980,765 + yeeZ BNILDI_14755 0.32 +0.5 2,980,765 + yeeZ BNILDI_14755 0.32 +0.6 2,980,775 - yeeZ BNILDI_14755 0.33 +0.5 2,980,808 + yeeZ BNILDI_14755 0.37 +1.5 2,980,820 + yeeZ BNILDI_14755 0.39 +0.1 2,980,877 + yeeZ BNILDI_14755 0.46 +0.5
Or see this region's nucleotide sequence