Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisC and hisD overlap by 4 nucleotides hisD and hisG are separated by 5 nucleotides
BNILDI_14735: hisC - histidinol-phosphate transaminase, at 2,976,602 to 2,977,672
hisC
BNILDI_14740: hisD - Histidinol dehydrogenase, at 2,977,669 to 2,978,973
hisD
BNILDI_14745: hisG - ATP phosphoribosyltransferase, at 2,978,979 to 2,979,878
hisG
Position (kb)
2977
2978
2979 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2976.697 kb on + strand at 2976.716 kb on - strand, within hisC at 2976.760 kb on + strand, within hisC at 2976.857 kb on - strand, within hisC at 2977.109 kb on + strand, within hisC at 2977.112 kb on - strand, within hisC at 2977.113 kb on + strand, within hisC at 2977.113 kb on + strand, within hisC at 2977.113 kb on + strand, within hisC at 2977.114 kb on - strand, within hisC at 2977.152 kb on - strand, within hisC at 2977.221 kb on + strand, within hisC at 2977.221 kb on + strand, within hisC at 2977.336 kb on - strand, within hisC at 2977.465 kb on - strand, within hisC at 2977.531 kb on + strand, within hisC at 2977.531 kb on + strand, within hisC at 2977.646 kb on + strand at 2977.657 kb on + strand at 2977.657 kb on + strand at 2977.684 kb on - strand at 2977.781 kb on - strand at 2977.860 kb on - strand, within hisD at 2978.024 kb on - strand, within hisD at 2978.549 kb on - strand, within hisD at 2978.584 kb on - strand, within hisD at 2978.585 kb on + strand, within hisD at 2978.586 kb on - strand, within hisD at 2978.586 kb on - strand, within hisD at 2978.586 kb on - strand, within hisD at 2978.586 kb on - strand, within hisD at 2978.647 kb on + strand, within hisD at 2978.911 kb on + strand at 2978.938 kb on - strand at 2978.943 kb on + strand at 2978.943 kb on + strand at 2978.943 kb on + strand at 2979.090 kb on + strand, within hisG at 2979.196 kb on + strand, within hisG at 2979.440 kb on + strand, within hisG at 2979.466 kb on + strand, within hisG at 2979.500 kb on + strand, within hisG at 2979.501 kb on - strand, within hisG at 2979.596 kb on + strand, within hisG at 2979.602 kb on - strand, within hisG at 2979.602 kb on - strand, within hisG at 2979.788 kb on + strand, within hisG at 2979.828 kb on + strand at 2979.828 kb on + strand at 2979.853 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,976,697 + -5.8 2,976,716 - hisC BNILDI_14735 0.11 -4.1 2,976,760 + hisC BNILDI_14735 0.15 -2.9 2,976,857 - hisC BNILDI_14735 0.24 -4.2 2,977,109 + hisC BNILDI_14735 0.47 -3.8 2,977,112 - hisC BNILDI_14735 0.48 -4.1 2,977,113 + hisC BNILDI_14735 0.48 -5.7 2,977,113 + hisC BNILDI_14735 0.48 -0.4 2,977,113 + hisC BNILDI_14735 0.48 -3.7 2,977,114 - hisC BNILDI_14735 0.48 -1.2 2,977,152 - hisC BNILDI_14735 0.51 -5.1 2,977,221 + hisC BNILDI_14735 0.58 -3.2 2,977,221 + hisC BNILDI_14735 0.58 -2.7 2,977,336 - hisC BNILDI_14735 0.69 -3.8 2,977,465 - hisC BNILDI_14735 0.81 -3.1 2,977,531 + hisC BNILDI_14735 0.87 -2.1 2,977,531 + hisC BNILDI_14735 0.87 -5.0 2,977,646 + -4.8 2,977,657 + -3.5 2,977,657 + -4.0 2,977,684 - -0.9 2,977,781 - -1.4 2,977,860 - hisD BNILDI_14740 0.15 -2.6 2,978,024 - hisD BNILDI_14740 0.27 -5.2 2,978,549 - hisD BNILDI_14740 0.67 -2.5 2,978,584 - hisD BNILDI_14740 0.70 -3.7 2,978,585 + hisD BNILDI_14740 0.70 -3.9 2,978,586 - hisD BNILDI_14740 0.70 -1.5 2,978,586 - hisD BNILDI_14740 0.70 -1.6 2,978,586 - hisD BNILDI_14740 0.70 -3.4 2,978,586 - hisD BNILDI_14740 0.70 -2.5 2,978,647 + hisD BNILDI_14740 0.75 -6.4 2,978,911 + -5.1 2,978,938 - -1.4 2,978,943 + -5.4 2,978,943 + -4.6 2,978,943 + +0.8 2,979,090 + hisG BNILDI_14745 0.12 -4.2 2,979,196 + hisG BNILDI_14745 0.24 +0.8 2,979,440 + hisG BNILDI_14745 0.51 -5.1 2,979,466 + hisG BNILDI_14745 0.54 -3.6 2,979,500 + hisG BNILDI_14745 0.58 -2.9 2,979,501 - hisG BNILDI_14745 0.58 -3.1 2,979,596 + hisG BNILDI_14745 0.69 -2.3 2,979,602 - hisG BNILDI_14745 0.69 -1.5 2,979,602 - hisG BNILDI_14745 0.69 +0.2 2,979,788 + hisG BNILDI_14745 0.90 -3.3 2,979,828 + -3.0 2,979,828 + -5.3 2,979,853 - -4.0
Or see this region's nucleotide sequence