Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisB and hisC overlap by 1 nucleotides hisC and hisD overlap by 4 nucleotides
BNILDI_14730: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 2,975,535 to 2,976,602
hisB
BNILDI_14735: hisC - histidinol-phosphate transaminase, at 2,976,602 to 2,977,672
hisC
BNILDI_14740: hisD - Histidinol dehydrogenase, at 2,977,669 to 2,978,973
hisD
Position (kb)
2976
2977
2978 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2975.625 kb on + strand at 2975.683 kb on + strand, within hisB at 2975.748 kb on - strand, within hisB at 2975.760 kb on - strand, within hisB at 2975.760 kb on - strand, within hisB at 2975.760 kb on - strand, within hisB at 2976.091 kb on - strand, within hisB at 2976.091 kb on - strand, within hisB at 2976.231 kb on - strand, within hisB at 2976.231 kb on - strand, within hisB at 2976.590 kb on - strand at 2976.697 kb on + strand at 2976.716 kb on - strand, within hisC at 2976.760 kb on + strand, within hisC at 2976.857 kb on - strand, within hisC at 2977.109 kb on + strand, within hisC at 2977.112 kb on - strand, within hisC at 2977.113 kb on + strand, within hisC at 2977.113 kb on + strand, within hisC at 2977.113 kb on + strand, within hisC at 2977.114 kb on - strand, within hisC at 2977.152 kb on - strand, within hisC at 2977.221 kb on + strand, within hisC at 2977.221 kb on + strand, within hisC at 2977.336 kb on - strand, within hisC at 2977.465 kb on - strand, within hisC at 2977.531 kb on + strand, within hisC at 2977.531 kb on + strand, within hisC at 2977.646 kb on + strand at 2977.657 kb on + strand at 2977.657 kb on + strand at 2977.684 kb on - strand at 2977.781 kb on - strand at 2977.860 kb on - strand, within hisD at 2978.024 kb on - strand, within hisD at 2978.549 kb on - strand, within hisD at 2978.584 kb on - strand, within hisD at 2978.585 kb on + strand, within hisD at 2978.586 kb on - strand, within hisD at 2978.586 kb on - strand, within hisD at 2978.586 kb on - strand, within hisD at 2978.586 kb on - strand, within hisD at 2978.647 kb on + strand, within hisD
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,975,625 + -0.2 2,975,683 + hisB BNILDI_14730 0.14 -3.5 2,975,748 - hisB BNILDI_14730 0.20 -3.6 2,975,760 - hisB BNILDI_14730 0.21 -3.3 2,975,760 - hisB BNILDI_14730 0.21 -0.2 2,975,760 - hisB BNILDI_14730 0.21 +0.2 2,976,091 - hisB BNILDI_14730 0.52 -5.7 2,976,091 - hisB BNILDI_14730 0.52 -5.2 2,976,231 - hisB BNILDI_14730 0.65 -2.7 2,976,231 - hisB BNILDI_14730 0.65 -4.1 2,976,590 - -3.3 2,976,697 + -5.8 2,976,716 - hisC BNILDI_14735 0.11 -4.1 2,976,760 + hisC BNILDI_14735 0.15 -2.9 2,976,857 - hisC BNILDI_14735 0.24 -4.2 2,977,109 + hisC BNILDI_14735 0.47 -3.8 2,977,112 - hisC BNILDI_14735 0.48 -4.1 2,977,113 + hisC BNILDI_14735 0.48 -5.7 2,977,113 + hisC BNILDI_14735 0.48 -0.4 2,977,113 + hisC BNILDI_14735 0.48 -3.7 2,977,114 - hisC BNILDI_14735 0.48 -1.2 2,977,152 - hisC BNILDI_14735 0.51 -5.1 2,977,221 + hisC BNILDI_14735 0.58 -3.2 2,977,221 + hisC BNILDI_14735 0.58 -2.7 2,977,336 - hisC BNILDI_14735 0.69 -3.8 2,977,465 - hisC BNILDI_14735 0.81 -3.1 2,977,531 + hisC BNILDI_14735 0.87 -2.1 2,977,531 + hisC BNILDI_14735 0.87 -5.0 2,977,646 + -4.8 2,977,657 + -3.5 2,977,657 + -4.0 2,977,684 - -0.9 2,977,781 - -1.4 2,977,860 - hisD BNILDI_14740 0.15 -2.6 2,978,024 - hisD BNILDI_14740 0.27 -5.2 2,978,549 - hisD BNILDI_14740 0.67 -2.5 2,978,584 - hisD BNILDI_14740 0.70 -3.7 2,978,585 + hisD BNILDI_14740 0.70 -3.9 2,978,586 - hisD BNILDI_14740 0.70 -1.5 2,978,586 - hisD BNILDI_14740 0.70 -1.6 2,978,586 - hisD BNILDI_14740 0.70 -3.4 2,978,586 - hisD BNILDI_14740 0.70 -2.5 2,978,647 + hisD BNILDI_14740 0.75 -6.4
Or see this region's nucleotide sequence