Strain Fitness in Escherichia coli ECRC62 around BNILDI_14730

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisA and hisH overlap by 1 nucleotideshisH and hisB overlap by 1 nucleotideshisB and hisC overlap by 1 nucleotides BNILDI_14720: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 2,974,208 to 2,974,945 hisA BNILDI_14725: hisH - imidazole glycerol phosphate synthase subunit HisH, at 2,974,945 to 2,975,535 hisH BNILDI_14730: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 2,975,535 to 2,976,602 hisB BNILDI_14735: hisC - histidinol-phosphate transaminase, at 2,976,602 to 2,977,672 hisC Position (kb) 2975 2976 2977Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 2974.929 kb on - strandat 2974.929 kb on - strandat 2975.026 kb on - strand, within hisHat 2975.064 kb on + strand, within hisHat 2975.065 kb on - strand, within hisHat 2975.065 kb on - strand, within hisHat 2975.103 kb on - strand, within hisHat 2975.117 kb on + strand, within hisHat 2975.123 kb on + strand, within hisHat 2975.302 kb on + strand, within hisHat 2975.432 kb on - strand, within hisHat 2975.537 kb on - strandat 2975.625 kb on + strandat 2975.683 kb on + strand, within hisBat 2975.748 kb on - strand, within hisBat 2975.760 kb on - strand, within hisBat 2975.760 kb on - strand, within hisBat 2975.760 kb on - strand, within hisBat 2976.091 kb on - strand, within hisBat 2976.091 kb on - strand, within hisBat 2976.231 kb on - strand, within hisBat 2976.231 kb on - strand, within hisBat 2976.590 kb on - strandat 2976.697 kb on + strandat 2976.716 kb on - strand, within hisCat 2976.760 kb on + strand, within hisCat 2976.857 kb on - strand, within hisCat 2977.109 kb on + strand, within hisCat 2977.112 kb on - strand, within hisCat 2977.113 kb on + strand, within hisCat 2977.113 kb on + strand, within hisCat 2977.113 kb on + strand, within hisCat 2977.114 kb on - strand, within hisCat 2977.152 kb on - strand, within hisCat 2977.221 kb on + strand, within hisCat 2977.221 kb on + strand, within hisCat 2977.336 kb on - strand, within hisCat 2977.465 kb on - strand, within hisCat 2977.531 kb on + strand, within hisCat 2977.531 kb on + strand, within hisC

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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2,974,929 - -4.9
2,974,929 - -5.3
2,975,026 - hisH BNILDI_14725 0.14 -4.4
2,975,064 + hisH BNILDI_14725 0.20 -4.2
2,975,065 - hisH BNILDI_14725 0.20 -3.9
2,975,065 - hisH BNILDI_14725 0.20 -2.5
2,975,103 - hisH BNILDI_14725 0.27 -3.9
2,975,117 + hisH BNILDI_14725 0.29 -2.1
2,975,123 + hisH BNILDI_14725 0.30 -1.4
2,975,302 + hisH BNILDI_14725 0.60 -2.0
2,975,432 - hisH BNILDI_14725 0.82 -1.5
2,975,537 - -4.3
2,975,625 + -0.2
2,975,683 + hisB BNILDI_14730 0.14 -3.5
2,975,748 - hisB BNILDI_14730 0.20 -3.6
2,975,760 - hisB BNILDI_14730 0.21 -3.3
2,975,760 - hisB BNILDI_14730 0.21 -0.2
2,975,760 - hisB BNILDI_14730 0.21 +0.2
2,976,091 - hisB BNILDI_14730 0.52 -5.7
2,976,091 - hisB BNILDI_14730 0.52 -5.2
2,976,231 - hisB BNILDI_14730 0.65 -2.7
2,976,231 - hisB BNILDI_14730 0.65 -4.1
2,976,590 - -3.3
2,976,697 + -5.8
2,976,716 - hisC BNILDI_14735 0.11 -4.1
2,976,760 + hisC BNILDI_14735 0.15 -2.9
2,976,857 - hisC BNILDI_14735 0.24 -4.2
2,977,109 + hisC BNILDI_14735 0.47 -3.8
2,977,112 - hisC BNILDI_14735 0.48 -4.1
2,977,113 + hisC BNILDI_14735 0.48 -5.7
2,977,113 + hisC BNILDI_14735 0.48 -0.4
2,977,113 + hisC BNILDI_14735 0.48 -3.7
2,977,114 - hisC BNILDI_14735 0.48 -1.2
2,977,152 - hisC BNILDI_14735 0.51 -5.1
2,977,221 + hisC BNILDI_14735 0.58 -3.2
2,977,221 + hisC BNILDI_14735 0.58 -2.7
2,977,336 - hisC BNILDI_14735 0.69 -3.8
2,977,465 - hisC BNILDI_14735 0.81 -3.1
2,977,531 + hisC BNILDI_14735 0.87 -2.1
2,977,531 + hisC BNILDI_14735 0.87 -5.0

Or see this region's nucleotide sequence