Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisA and hisH overlap by 1 nucleotides hisH and hisB overlap by 1 nucleotides hisB and hisC overlap by 1 nucleotides
BNILDI_14720: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 2,974,208 to 2,974,945
hisA
BNILDI_14725: hisH - imidazole glycerol phosphate synthase subunit HisH, at 2,974,945 to 2,975,535
hisH
BNILDI_14730: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 2,975,535 to 2,976,602
hisB
BNILDI_14735: hisC - histidinol-phosphate transaminase, at 2,976,602 to 2,977,672
hisC
Position (kb)
2975
2976
2977 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2974.929 kb on - strand at 2974.929 kb on - strand at 2975.026 kb on - strand, within hisH at 2975.064 kb on + strand, within hisH at 2975.065 kb on - strand, within hisH at 2975.065 kb on - strand, within hisH at 2975.103 kb on - strand, within hisH at 2975.117 kb on + strand, within hisH at 2975.123 kb on + strand, within hisH at 2975.302 kb on + strand, within hisH at 2975.432 kb on - strand, within hisH at 2975.537 kb on - strand at 2975.625 kb on + strand at 2975.683 kb on + strand, within hisB at 2975.748 kb on - strand, within hisB at 2975.760 kb on - strand, within hisB at 2975.760 kb on - strand, within hisB at 2975.760 kb on - strand, within hisB at 2976.091 kb on - strand, within hisB at 2976.091 kb on - strand, within hisB at 2976.231 kb on - strand, within hisB at 2976.231 kb on - strand, within hisB at 2976.590 kb on - strand at 2976.697 kb on + strand at 2976.716 kb on - strand, within hisC at 2976.760 kb on + strand, within hisC at 2976.857 kb on - strand, within hisC at 2977.109 kb on + strand, within hisC at 2977.112 kb on - strand, within hisC at 2977.113 kb on + strand, within hisC at 2977.113 kb on + strand, within hisC at 2977.113 kb on + strand, within hisC at 2977.114 kb on - strand, within hisC at 2977.152 kb on - strand, within hisC at 2977.221 kb on + strand, within hisC at 2977.221 kb on + strand, within hisC at 2977.336 kb on - strand, within hisC at 2977.465 kb on - strand, within hisC at 2977.531 kb on + strand, within hisC at 2977.531 kb on + strand, within hisC
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,974,929 - -4.9 2,974,929 - -5.3 2,975,026 - hisH BNILDI_14725 0.14 -4.4 2,975,064 + hisH BNILDI_14725 0.20 -4.2 2,975,065 - hisH BNILDI_14725 0.20 -3.9 2,975,065 - hisH BNILDI_14725 0.20 -2.5 2,975,103 - hisH BNILDI_14725 0.27 -3.9 2,975,117 + hisH BNILDI_14725 0.29 -2.1 2,975,123 + hisH BNILDI_14725 0.30 -1.4 2,975,302 + hisH BNILDI_14725 0.60 -2.0 2,975,432 - hisH BNILDI_14725 0.82 -1.5 2,975,537 - -4.3 2,975,625 + -0.2 2,975,683 + hisB BNILDI_14730 0.14 -3.5 2,975,748 - hisB BNILDI_14730 0.20 -3.6 2,975,760 - hisB BNILDI_14730 0.21 -3.3 2,975,760 - hisB BNILDI_14730 0.21 -0.2 2,975,760 - hisB BNILDI_14730 0.21 +0.2 2,976,091 - hisB BNILDI_14730 0.52 -5.7 2,976,091 - hisB BNILDI_14730 0.52 -5.2 2,976,231 - hisB BNILDI_14730 0.65 -2.7 2,976,231 - hisB BNILDI_14730 0.65 -4.1 2,976,590 - -3.3 2,976,697 + -5.8 2,976,716 - hisC BNILDI_14735 0.11 -4.1 2,976,760 + hisC BNILDI_14735 0.15 -2.9 2,976,857 - hisC BNILDI_14735 0.24 -4.2 2,977,109 + hisC BNILDI_14735 0.47 -3.8 2,977,112 - hisC BNILDI_14735 0.48 -4.1 2,977,113 + hisC BNILDI_14735 0.48 -5.7 2,977,113 + hisC BNILDI_14735 0.48 -0.4 2,977,113 + hisC BNILDI_14735 0.48 -3.7 2,977,114 - hisC BNILDI_14735 0.48 -1.2 2,977,152 - hisC BNILDI_14735 0.51 -5.1 2,977,221 + hisC BNILDI_14735 0.58 -3.2 2,977,221 + hisC BNILDI_14735 0.58 -2.7 2,977,336 - hisC BNILDI_14735 0.69 -3.8 2,977,465 - hisC BNILDI_14735 0.81 -3.1 2,977,531 + hisC BNILDI_14735 0.87 -2.1 2,977,531 + hisC BNILDI_14735 0.87 -5.0
Or see this region's nucleotide sequence