Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisF and hisA overlap by 19 nucleotides hisA and hisH overlap by 1 nucleotides hisH and hisB overlap by 1 nucleotides
BNILDI_14715: hisF - Imidazole glycerol phosphate synthase subunit HisF, at 2,973,450 to 2,974,226
hisF
BNILDI_14720: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 2,974,208 to 2,974,945
hisA
BNILDI_14725: hisH - imidazole glycerol phosphate synthase subunit HisH, at 2,974,945 to 2,975,535
hisH
BNILDI_14730: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 2,975,535 to 2,976,602
hisB
Position (kb)
2974
2975
2976 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2973.978 kb on - strand, within hisF at 2974.073 kb on + strand, within hisF at 2974.165 kb on - strand at 2974.165 kb on - strand at 2974.322 kb on - strand, within hisA at 2974.386 kb on + strand, within hisA at 2974.387 kb on - strand, within hisA at 2974.442 kb on + strand, within hisA at 2974.929 kb on - strand at 2974.929 kb on - strand at 2975.026 kb on - strand, within hisH at 2975.064 kb on + strand, within hisH at 2975.065 kb on - strand, within hisH at 2975.065 kb on - strand, within hisH at 2975.103 kb on - strand, within hisH at 2975.117 kb on + strand, within hisH at 2975.123 kb on + strand, within hisH at 2975.302 kb on + strand, within hisH at 2975.432 kb on - strand, within hisH at 2975.537 kb on - strand at 2975.625 kb on + strand at 2975.683 kb on + strand, within hisB at 2975.748 kb on - strand, within hisB at 2975.760 kb on - strand, within hisB at 2975.760 kb on - strand, within hisB at 2975.760 kb on - strand, within hisB at 2976.091 kb on - strand, within hisB at 2976.091 kb on - strand, within hisB at 2976.231 kb on - strand, within hisB at 2976.231 kb on - strand, within hisB
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,973,978 - hisF BNILDI_14715 0.68 -4.5 2,974,073 + hisF BNILDI_14715 0.80 +0.2 2,974,165 - -4.6 2,974,165 - -5.0 2,974,322 - hisA BNILDI_14720 0.15 -2.8 2,974,386 + hisA BNILDI_14720 0.24 -3.1 2,974,387 - hisA BNILDI_14720 0.24 -2.5 2,974,442 + hisA BNILDI_14720 0.32 -3.2 2,974,929 - -4.9 2,974,929 - -5.3 2,975,026 - hisH BNILDI_14725 0.14 -4.4 2,975,064 + hisH BNILDI_14725 0.20 -4.2 2,975,065 - hisH BNILDI_14725 0.20 -3.9 2,975,065 - hisH BNILDI_14725 0.20 -2.5 2,975,103 - hisH BNILDI_14725 0.27 -3.9 2,975,117 + hisH BNILDI_14725 0.29 -2.1 2,975,123 + hisH BNILDI_14725 0.30 -1.4 2,975,302 + hisH BNILDI_14725 0.60 -2.0 2,975,432 - hisH BNILDI_14725 0.82 -1.5 2,975,537 - -4.3 2,975,625 + -0.2 2,975,683 + hisB BNILDI_14730 0.14 -3.5 2,975,748 - hisB BNILDI_14730 0.20 -3.6 2,975,760 - hisB BNILDI_14730 0.21 -3.3 2,975,760 - hisB BNILDI_14730 0.21 -0.2 2,975,760 - hisB BNILDI_14730 0.21 +0.2 2,976,091 - hisB BNILDI_14730 0.52 -5.7 2,976,091 - hisB BNILDI_14730 0.52 -5.2 2,976,231 - hisB BNILDI_14730 0.65 -2.7 2,976,231 - hisB BNILDI_14730 0.65 -4.1
Or see this region's nucleotide sequence