Strain Fitness in Escherichia coli ECRC62 around BNILDI_14720

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisIE and hisF overlap by 7 nucleotideshisF and hisA overlap by 19 nucleotideshisA and hisH overlap by 1 nucleotideshisH and hisB overlap by 1 nucleotides BNILDI_14710: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 2,972,845 to 2,973,456 hisIE BNILDI_14715: hisF - Imidazole glycerol phosphate synthase subunit HisF, at 2,973,450 to 2,974,226 hisF BNILDI_14720: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 2,974,208 to 2,974,945 hisA BNILDI_14725: hisH - imidazole glycerol phosphate synthase subunit HisH, at 2,974,945 to 2,975,535 hisH BNILDI_14730: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 2,975,535 to 2,976,602 hisB Position (kb) 2974 2975Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2973.228 kb on + strand, within hisIEat 2973.236 kb on - strand, within hisIEat 2973.348 kb on + strand, within hisIEat 2973.349 kb on - strand, within hisIEat 2973.351 kb on - strand, within hisIEat 2973.487 kb on + strandat 2973.496 kb on - strandat 2973.809 kb on - strand, within hisFat 2973.813 kb on - strand, within hisFat 2973.854 kb on + strand, within hisFat 2973.854 kb on + strand, within hisFat 2973.891 kb on + strand, within hisFat 2973.978 kb on - strand, within hisFat 2974.073 kb on + strand, within hisFat 2974.165 kb on - strandat 2974.165 kb on - strandat 2974.322 kb on - strand, within hisAat 2974.386 kb on + strand, within hisAat 2974.387 kb on - strand, within hisAat 2974.442 kb on + strand, within hisAat 2974.929 kb on - strandat 2974.929 kb on - strandat 2975.026 kb on - strand, within hisHat 2975.064 kb on + strand, within hisHat 2975.065 kb on - strand, within hisHat 2975.065 kb on - strand, within hisHat 2975.103 kb on - strand, within hisHat 2975.117 kb on + strand, within hisHat 2975.123 kb on + strand, within hisHat 2975.302 kb on + strand, within hisHat 2975.432 kb on - strand, within hisHat 2975.537 kb on - strandat 2975.625 kb on + strandat 2975.683 kb on + strand, within hisBat 2975.748 kb on - strand, within hisBat 2975.760 kb on - strand, within hisBat 2975.760 kb on - strand, within hisBat 2975.760 kb on - strand, within hisB

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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2,973,228 + hisIE BNILDI_14710 0.63 -3.1
2,973,236 - hisIE BNILDI_14710 0.64 -3.4
2,973,348 + hisIE BNILDI_14710 0.82 -3.9
2,973,349 - hisIE BNILDI_14710 0.82 -5.6
2,973,351 - hisIE BNILDI_14710 0.83 -4.6
2,973,487 + -3.8
2,973,496 - -2.8
2,973,809 - hisF BNILDI_14715 0.46 -4.9
2,973,813 - hisF BNILDI_14715 0.47 -2.9
2,973,854 + hisF BNILDI_14715 0.52 -3.6
2,973,854 + hisF BNILDI_14715 0.52 -2.5
2,973,891 + hisF BNILDI_14715 0.57 -1.9
2,973,978 - hisF BNILDI_14715 0.68 -4.5
2,974,073 + hisF BNILDI_14715 0.80 +0.2
2,974,165 - -4.6
2,974,165 - -5.0
2,974,322 - hisA BNILDI_14720 0.15 -2.8
2,974,386 + hisA BNILDI_14720 0.24 -3.1
2,974,387 - hisA BNILDI_14720 0.24 -2.5
2,974,442 + hisA BNILDI_14720 0.32 -3.2
2,974,929 - -4.9
2,974,929 - -5.3
2,975,026 - hisH BNILDI_14725 0.14 -4.4
2,975,064 + hisH BNILDI_14725 0.20 -4.2
2,975,065 - hisH BNILDI_14725 0.20 -3.9
2,975,065 - hisH BNILDI_14725 0.20 -2.5
2,975,103 - hisH BNILDI_14725 0.27 -3.9
2,975,117 + hisH BNILDI_14725 0.29 -2.1
2,975,123 + hisH BNILDI_14725 0.30 -1.4
2,975,302 + hisH BNILDI_14725 0.60 -2.0
2,975,432 - hisH BNILDI_14725 0.82 -1.5
2,975,537 - -4.3
2,975,625 + -0.2
2,975,683 + hisB BNILDI_14730 0.14 -3.5
2,975,748 - hisB BNILDI_14730 0.20 -3.6
2,975,760 - hisB BNILDI_14730 0.21 -3.3
2,975,760 - hisB BNILDI_14730 0.21 -0.2
2,975,760 - hisB BNILDI_14730 0.21 +0.2

Or see this region's nucleotide sequence